LOCUS       32_pQE-30               4569 bp ds-DNA     circular     11-FEB-2019
DEFINITION  .
KEYWORDS    "creator:Barbora" "marker:AmpR, CmR"                               
FEATURES             Location/Qualifiers
     protein_bind    1703..1719
                     /label="lac operator"
     misc_feature    4060..4200
                     /label="bom"
     promoter        1689..1695
                     /label="T5 promoter"
     promoter        complement(1437..1541)
                     /label="AmpR promoter"
     CDS             complement(1368..1436)
                     /label="AmpR"
     promoter        1666..1671
                     /label="T5 promoter"
     terminator      2957..3051
                     /label="lambda t0 terminator"
     terminator      3819..3905
                     /label="rrnB T1 terminator"
     CDS             complement(576..1367)
                     /label="AmpR"
     CDS             3095..3754
                     /label="CmR"
     CDS             1768..1785
                     /label="6xHis"
     rep_origin      complement(join(4386..4569,1..405))
                     /label="ori"
     promoter        1651..1665
                     /label="T5 promoter"
     misc_feature    1790..2936
                     /label="CK"
     RBS             1738..1749
                     /label="RBS"
     promoter        1672..1688
                     /label="T5 promoter"
     protein_bind    1671..1687
                     /label="lac operator"
ORIGIN
        1 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
       61 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      121 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      181 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      241 TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC
      301 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT
      361 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC
      421 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
      481 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
      541 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
      601 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
      661 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC
      721 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
      781 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
      841 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
      901 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
      961 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     1021 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     1081 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     1141 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     1201 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     1261 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     1321 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     1381 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     1441 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     1501 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
     1561 CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC
     1621 ACGAGGCCCT TTCGTCTTCA CCTCGAGAAA TCATAAAAAA TTTATTTGCT TTGTGAGCGG
     1681 ATAACAATTA TAATAGATTC AATTGTGAGC GGATAACAAT TTCACACAGA ATTCATTAAA
     1741 GAGGAGAAAT TAACTATGAG AGGATCGCAT CACCATCACC ATCACGGATC CATGCCGTTC
     1801 AGCAGCACCC ACAACAAACA CAAGCTGAAG TTCTCAGCCG AGGAGGAATT CCCGGACCTC
     1861 TCGAAGCACA ACAACCACAT GGCCAAAGTC CTCACCCCGG AGCTCTACAA ACGCCTCCGG
     1921 GATAAGGAGA CCCCGAGCGG ATTCACCCTC GACGACGTCA TCCAAACCGG GGTCGATAAC
     1981 CCCGGTCACC CATTCATCAT GACGGTGGGC TGCGTGGCGG GGGACGAGGA GTCCTACGAG
     2041 GTGTTCAAGG ATCTCTTCGA CCCCGTCATC CAGGACCGCC ACGGGGGGTA CAAACCCACC
     2101 GATAAGCACC GCACCGACCT CAACCACGAG AACCTCAAGG GGGGTGACGA CCTGGACCCC
     2161 AAATACGTGC TGAGCAGCCG CGTGCGCACG GGGAGAAGCA TTAAGGGGTA CTCCCTGCCC
     2221 CCACACTGCA GCCGTGGGGA GCGGCGCGCC GTCGAGAAGC TGTCCGTGGA AGCCCTGAAC
     2281 AGCCTGGAGG GGGAGTTCAA GGGCCGCTAT TACCCTCTGA AGGCCATGAC GGAGCAGGAG
     2341 CAGCAGCAGC TGATCGACGA CCACTTCCTG TTCGATAAAC CCGTCTCCCC ACTGCTGCTC
     2401 GCATCCGGGA TGGCCCGAGA TTGGCCCGAC GCCAGGGGCA TCTGGCACAA CGACAACAAG
     2461 ACGTTCCTGG TGTGGGTGAA TGAGGAGGAC CACCTGAGGG TCATCTCCAT GGAGAAGGGA
     2521 GGAAATATGA AGGAGGTCTT CCGGCGCTTC TGCGTCGGCC TCAAGAAGAT CGAGGAGATC
     2581 TTCAAGAAGG CCGGGCACCC CTTCATGTGG ACGGAGCACC TGGGTTACAT CCTGACGTGC
     2641 CCCTCCAATT TGGGCACGGG GCTGCGGGGG GGGGTCCACG TGAAGCTCCC CAAACTCAGC
     2701 CAGCACCCCA AATTCGAGGA GATCCTCCAT AGGCTGCGCC TGCAGAAACG GGGCACCGGC
     2761 GGGGTGGATA CGGCGGCGGT GGGCGCCGTC TTTGACATCT CCAACGCCGA CCGGCTGGGC
     2821 TTCTCGGAGG TGGAGCAGGT GCAGATGGTG GTGGACGGCG TCAAGCTCAT GGTGGAGATG
     2881 GAGAAGAAGC TGGAGCAGAA CCAGCCCATA GACGACATGA TCCCGGCCCA GAAGTAAGCT
     2941 TAATTAGCTG AGCTTGGACT CCTGTTGATA GATCCAGTAA TGACCTCAGA ACTCCATCTG
     3001 GATTTGTTCA GAACGCTCGG TTGCCGCCGG GCGTTTTTTA TTGGTGAGAA TCCAAGCTAG
     3061 CTTGGCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC
     3121 CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC
     3181 TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA
     3241 GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC
     3301 TCATCCGGAA TTTCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA
     3361 CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA
     3421 CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA
     3481 AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC
     3541 CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC
     3601 CGTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT
     3661 TCAGGTTCAT CATGCCGTTT GTGATGGCTT CCATGTCGGC AGAATGCTTA ATGAATTACA
     3721 ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT TAAGGCAGTT ATTGGTGCCC
     3781 TTAAACGCCT GGGGTAATGA CTCTCTAGCT TGAGGCATCA AATAAAACGA AAGGCTCAGT
     3841 CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC CTGAGTAGGA
     3901 CAAATCCGCC CTCTAGAGCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA
     3961 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC
     4021 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA CCCAGTCACG
     4081 TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA
     4141 GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG
     4201 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG
     4261 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA
     4321 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
     4381 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
     4441 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
     4501 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
     4561 GGAAGCGTG
//