LOCUS Exported 5820 bp ds-DNA circular SYN 19-FEB-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5820) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Feb 19, 2019 from SnapGene Viewer 4.3.1 https://www.snapgene.com FEATURES Location/Qualifiers source 1..5820 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(280..868) /direction=LEFT /label=ori CDS 990..1805 /codon_start=1 /label=KanR /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin complement(1898..2353) /direction=LEFT /label=f1 ori terminator 2390..2437 /label=T7 terminator CDS complement(2501..3109) /codon_start=1 /label=AK1 /translation="MSTEKLKHHKIIFVVGGPGSGKGTQCEKIVHKYGYTHLSTGDLLR AEVSSGSERGKKLQAIMEKGELVPLDTVLDMLRDAMLAKADTSKGFLIDGYPREVKQGE EFEKKIAPPTLLLYVDAGKETMVKRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIA FYKGRGIVRQLNAEGTVDEVFQQVCSYLDKLLEHHHHHH" CDS complement(2504..2521) /codon_start=1 /label=6x His Tag /translation="HHHHHH" misc_binding complement(3156..3178) /label=LacO primer_bind complement(3178..3197) /label=T7 promoter 3510..3587 /label=lacI promoter CDS 3588..4670 /codon_start=1 /label=lacI /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" CDS 5479..5670 /codon_start=1 /label=rop /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" misc_feature join(5772..5820,1..94) /label=bom ORIGIN 1 actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt gtgaaatacc 61 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga 121 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 181 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 241 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 301 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 361 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 421 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 481 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 541 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 601 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 661 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 721 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 781 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 841 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 901 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgaacaata aaactgtctg 961 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct 1021 ctaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg 1081 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag 1141 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca 1201 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc 1261 ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc caggtattag 1321 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt 1381 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc 1441 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta 1501 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg 1561 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat 1621 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca 1681 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat 1741 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt 1801 tctaagaatt aattcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 1861 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg 1921 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 1981 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 2041 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 2101 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 2161 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 2221 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 2281 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 2341 ctacagggcg cgtcccattc gccaatccgg atatagttcc tcctttcagc aaaaaacccc 2401 tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag 2461 ccaactcagc ttcctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt 2521 gctcgagcag cttgtcgagg taggagcaga cctgctggaa aacctcatcc acggtgccct 2581 cggcattcag ctggcggacg atgccccggc ccttgtagaa ggcgatgacg ggttcggtag 2641 ccttgtagta cgtctccaag cgcttcttga tggtctcctc gttgtcgtcc acccgcccgc 2701 tggtctctcc ccgcttcagc agccgcttca ccatcgtctc cttccccgca tccacgtaga 2761 gcagcagcgt cgggggggca atcttctttt caaactcctc tccctgcttc acctcgcgag 2821 ggtagccgtc aatgaggaaa cccttggagg tgtctgcctt agccaacatg gcgtcccgca 2881 gcatgtccag caccgtgtcc aggggaacca gctcgccctt ctccatgatg gcttgcagct 2941 tcttgccccg ctctgagccc gagctgacct ctgcccggag caggtcccca gtggagaggt 3001 gagtgtaccc atacttgtgc acaatcttct cgcattgcgt ccccttccct gagccggggc 3061 cacccaccac aaagatgatc ttgtggtgct tcagtttttc tgtcgacata tgtatatctc 3121 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc 3181 tatagtgagt cgtattaatt tcgcgggatc gagatcgatc tcgatcctct acgccggacg 3241 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat 3301 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg 3361 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt 3421 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga 3481 gtcgcataag ggagagcgtc gagatcccgg acaccatcga atggcgcaaa acctttcgcg 3541 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa 3601 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga 3661 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc 3721 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg 3781 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat 3841 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg 3901 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta 3961 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg 4021 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag 4081 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt 4141 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc 4201 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg 4261 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg 4321 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg 4381 gtgcggacat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc 4441 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc 4501 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga 4561 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 4621 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 4681 attaatgtaa gttagctcac tcattaggca ccgggatctc gaccgatgcc cttgagagcc 4741 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg 4801 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc 4861 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga 4921 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag 4981 aagcaggcca ttatcgccgg catggcggcc ccacgggtgc gcatgatcgt gctcctgtcg 5041 ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata 5101 cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga 5161 atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc 5221 attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct 5281 gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat 5341 accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc 5401 cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc 5461 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt 5521 atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag 5581 gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg 5641 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 5701 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 5761 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta //