LOCUS       Exported                5820 bp ds-DNA     circular SYN 19-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 19, 2019 from SnapGene Viewer 4.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(280..868)
                     /direction=LEFT
                     /label=ori
     CDS             990..1805
                     /codon_start=1
                     /label=KanR
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(1898..2353)
                     /direction=LEFT
                     /label=f1 ori
     terminator      2390..2437
                     /label=T7 terminator
     CDS             complement(2501..3109)
                     /codon_start=1
                     /label=AK1
                     /translation="MSTEKLKHHKIIFVVGGPGSGKGTQCEKIVHKYGYTHLSTGDLLR
                     AEVSSGSERGKKLQAIMEKGELVPLDTVLDMLRDAMLAKADTSKGFLIDGYPREVKQGE
                     EFEKKIAPPTLLLYVDAGKETMVKRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIA
                     FYKGRGIVRQLNAEGTVDEVFQQVCSYLDKLLEHHHHHH"
     CDS             complement(2504..2521)
                     /codon_start=1
                     /label=6x His Tag
                     /translation="HHHHHH"
     misc_binding    complement(3156..3178)
                     /label=LacO
     primer_bind     complement(3178..3197)
                     /label=T7
     promoter        3510..3587
                     /label=lacI promoter
     CDS             3588..4670
                     /codon_start=1
                     /label=lacI
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             5479..5670
                     /codon_start=1
                     /label=rop
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    join(5772..5820,1..94)
                     /label=bom
ORIGIN
        1 actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt gtgaaatacc
       61 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
      121 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      181 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      241 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      301 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      361 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      421 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      481 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      541 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      601 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      661 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
      721 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
      781 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
      841 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
      901 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgaacaata aaactgtctg
      961 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct
     1021 ctaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     1081 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag
     1141 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     1201 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     1261 ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc caggtattag
     1321 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     1381 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     1441 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     1501 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     1561 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     1621 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     1681 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     1741 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     1801 tctaagaatt aattcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     1861 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg
     1921 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     1981 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     2041 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     2101 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     2161 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     2221 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     2281 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     2341 ctacagggcg cgtcccattc gccaatccgg atatagttcc tcctttcagc aaaaaacccc
     2401 tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag
     2461 ccaactcagc ttcctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt
     2521 gctcgagcag cttgtcgagg taggagcaga cctgctggaa aacctcatcc acggtgccct
     2581 cggcattcag ctggcggacg atgccccggc ccttgtagaa ggcgatgacg ggttcggtag
     2641 ccttgtagta cgtctccaag cgcttcttga tggtctcctc gttgtcgtcc acccgcccgc
     2701 tggtctctcc ccgcttcagc agccgcttca ccatcgtctc cttccccgca tccacgtaga
     2761 gcagcagcgt cgggggggca atcttctttt caaactcctc tccctgcttc acctcgcgag
     2821 ggtagccgtc aatgaggaaa cccttggagg tgtctgcctt agccaacatg gcgtcccgca
     2881 gcatgtccag caccgtgtcc aggggaacca gctcgccctt ctccatgatg gcttgcagct
     2941 tcttgccccg ctctgagccc gagctgacct ctgcccggag caggtcccca gtggagaggt
     3001 gagtgtaccc atacttgtgc acaatcttct cgcattgcgt ccccttccct gagccggggc
     3061 cacccaccac aaagatgatc ttgtggtgct tcagtttttc tgtcgacata tgtatatctc
     3121 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc
     3181 tatagtgagt cgtattaatt tcgcgggatc gagatcgatc tcgatcctct acgccggacg
     3241 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     3301 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     3361 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     3421 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     3481 gtcgcataag ggagagcgtc gagatcccgg acaccatcga atggcgcaaa acctttcgcg
     3541 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa
     3601 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga
     3661 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc
     3721 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg
     3781 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat
     3841 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg
     3901 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta
     3961 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg
     4021 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag
     4081 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt
     4141 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc
     4201 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg
     4261 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg
     4321 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg
     4381 gtgcggacat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc
     4441 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
     4501 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga
     4561 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4621 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4681 attaatgtaa gttagctcac tcattaggca ccgggatctc gaccgatgcc cttgagagcc
     4741 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     4801 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     4861 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     4921 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
     4981 aagcaggcca ttatcgccgg catggcggcc ccacgggtgc gcatgatcgt gctcctgtcg
     5041 ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata
     5101 cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga
     5161 atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc
     5221 attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct
     5281 gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat
     5341 accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc
     5401 cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc
     5461 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     5521 atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag
     5581 gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg
     5641 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     5701 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     5761 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
//
