LOCUS       34_pQE-30               3883 bp ds-DNA     circular     18-FEB-2019
DEFINITION  .
KEYWORDS    "creator:Barbora" "marker:AmpR, CmR"                               
FEATURES             Location/Qualifiers
     CDS             complement(1324..1392)
                     /label="AmpR"
     misc_feature    1743..2206
                     /label="NDK"
     promoter        1607..1621
                     /label="T5 promoter"
     protein_bind    1627..1643
                     /label="lac operator"
     RBS             1694..1705
                     /label="RBS"
     rep_origin      complement(join(3656..3883,1..361))
                     /label="ori"
     misc_feature    3330..3470
                     /label="bom"
     promoter        1645..1651
                     /label="T5 promoter"
     terminator      3089..3175
                     /label="rrnB T1 terminator"
     promoter        complement(1393..1497)
                     /label="AmpR promoter"
     CDS             complement(532..1323)
                     /label="AmpR"
     protein_bind    1659..1675
                     /label="lac operator"
     CDS             2365..3024
                     /label="CmR"
     promoter        1622..1627
                     /label="T5 promoter"
     terminator      2227..2321
                     /label="lambda t0 terminator"
     promoter        1628..1644
                     /label="T5 promoter"
     CDS             1724..1741
                     /label="6xHis"
ORIGIN
        1 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
       61 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
      121 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
      181 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT
      241 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
      301 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
      361 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      421 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      481 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      541 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
      601 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
      661 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
      721 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
      781 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
      841 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
      901 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
      961 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1021 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1081 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1141 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1201 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1261 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1321 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1381 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1441 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1501 ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA
     1561 TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCACCTC GAGAAATCAT AAAAAATTTA
     1621 TTTGCTTTGT GAGCGGATAA CAATTATAAT AGATTCAATT GTGAGCGGAT AACAATTTCA
     1681 CACAGAATTC ATTAAAGAGG AGAAATTAAC TATGAGAGGA TCGCATCACC ATCACCATCA
     1741 CGGATCCGAT GACGATGACA AAGCTATTGA ACGTACTTTT TCCATCATCA AACCGAACGC
     1801 GGTAGCAAAA AACGTCATTG GTAATATCTT TGCGCGCTTT GAAGCTGCAG GGTTCAAAAT
     1861 TGTTGGCACC AAAATGCTGC ACCTGACCGT TGAACAGGCA CGTGGCTTTT ATGCTGAACA
     1921 CGATGGAAAA CCGTTCTTTG ATGGTCTGGT TGAATTCATG ACCTCTGGCC CGATCGTGGT
     1981 TTCCGTGCTG GAAGGTGAAA ACGCCGTTCA GCGTCACCGC GATCTGCTGG GCGCGACCAA
     2041 TCCGGCAAAC GCACTGGCTG GTACTCTGCG CGCTGATTAC GCTGACAGCC TGACCGAAAA
     2101 CGGTACCCAC GGTTCTGATT CCGTCGAATC TGCCGCTCGC GAAATCGCTT ATTTCTTTGG
     2161 CGAAGGCGAA GTGTGCCCGC GCACCCGTTA AGTCGACCTG CAGCCaAGCT TAATTAGCTG
     2221 AGCTTGGACT CCTGTTGATA GATCCAGTAA TGACCTCAGA ACTCCATCTG GATTTGTTCA
     2281 GAACGCTCGG TTGCCGCCGG GCGTTTTTTA TTGGTGAGAA TCCAAGCTAG CTTGGCGAGA
     2341 TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT
     2401 ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC
     2461 TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG
     2521 CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA
     2581 TTTCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC
     2641 ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT
     2701 TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC
     2761 TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT
     2821 TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC CGTTTTCACC
     2881 ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT TCAGGTTCAT
     2941 CATGCCGTTT GTGATGGCTT CCATGTCGGC AGAATGCTTA ATGAATTACA ACAGTACTGC
     3001 GATGAGTGGC AGGGCGGGGC GTAATTTTTT TAAGGCAGTT ATTGGTGCCC TTAAACGCCT
     3061 GGGGTAATGA CTCTCTAGCT TGAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG
     3121 GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC
     3181 CTCTAGAGCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC
     3241 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC
     3301 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA CCCAGTCACG TAGCGATAGC
     3361 GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA
     3421 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCTCTTCCG
     3481 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
     3541 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
     3601 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
     3661 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
     3721 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
     3781 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
     3841 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTA
//