LOCUS Exported 7386 bp ds-DNA circular SYN 27-OCT-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7386) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..7386 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(135..441) /direction=LEFT /label=f1(-)ori primer_bind 622..643 /label=T7 misc_signal complement(656..968) /label=PB 5' TR /label=PB 5' TR(1) misc_signal complement(656..802) /label=PB 5' TR promoter 1052..2514 /label=CAG promoter CDS 2721..3464 /label=rtTA-M2 CDS 2721..3341 /label=rTetR CDS 3342..3458 /label=Min.VP16ADs misc_signal 3512..4084 /label=EMCV IRES CDS 4088..4879 /label=Neo polyA_signal 4883..4937 /label=SV40 pA misc_signal 4944..5178 /label=PB 3' TR primer_bind complement(5197..5216) /label=T3 rep_origin complement(5621..6303) /direction=LEFT /label=ColE1 ori CDS complement(6401..7060) /label=AmpR ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac 661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt 721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc 781 cgtgaggcgt gcttgtcaat gaggtaagtg tcactgattt tgaactataa cgaccgcgtg 841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat 901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta 961 tagatatcgt cgacattgat tattgactag ctagttatta atagtaatca attacggggt 1021 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 1081 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 1141 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 1201 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 1261 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 1321 agtacatcta cgtattagtc atcgctatta ccatgatggt cgaggtgagc cccacgttct 1381 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt 1441 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg 1501 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg 1561 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc 1621 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg 1681 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc 1741 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt 1801 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg 1861 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc 1921 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg 1981 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag 2041 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc 2101 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg 2161 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg 2221 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg 2281 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt 2341 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg 2401 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga 2461 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc 2521 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt 2581 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct 2641 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca 2701 aagaattcct cgaggccacc atgtctagac tggacaagag caaagtcata aacggcgctc 2761 tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc 2821 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg 2881 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt 2941 catggcaaga ctttctgcgg aacaacgcca agtcattccg ctgtgctctc ctctcacatc 3001 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg 3061 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc 3121 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag 3181 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa 3241 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca 3301 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg 3361 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc 3421 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaagcg gccgctcgat 3481 aagcttgata tcgaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc 3541 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct 3601 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt 3661 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct 3721 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc 3781 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag 3841 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc 3901 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga 3961 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt 4021 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc 4081 cacaaccatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 4141 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 4201 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 4261 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 4321 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 4381 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 4441 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 4501 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 4561 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat 4621 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 4681 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 4741 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga 4801 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 4861 ccttcttgac gagttcttct gaggggatcg gcaataaaaa gacagaataa aacgcacggg 4921 tgttgggtcg tttgttcgga tcctttgtta ctttatagaa gaaattttga gtttttgttt 4981 ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat tagtatgtaa 5041 gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc gatatacaga 5101 ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt cacaatatga 5161 ttatctttct agggttaaag agctccagct tttgttccct ttagtgaggg ttaatttcga 5221 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 5281 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 5341 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 5401 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 5461 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 5521 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 5581 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 5641 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 5701 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 5761 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 5821 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 5881 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 5941 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 6001 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 6061 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 6121 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 6181 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 6241 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 6301 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 6361 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 6421 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 6481 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 6541 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 6601 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 6661 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 6721 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 6781 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 6841 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 6901 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 6961 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 7021 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 7081 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 7141 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 7201 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 7261 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 7321 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 7381 tgccac //