LOCUS Exported 8038 bp ds-DNA circular SYN 12-MAR-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8038) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..8038 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(135..441) /direction=LEFT /label=f1(-)ori primer_bind 622..643 /label=T7 misc_signal complement(656..968) /label=PB 5' TR promoter 1052..2654 /label=CAG promoter CDS 2721..3464 /label=rtTA-M2 CDS 2721..3341 /label=rTetR CDS 3342..3458 /label=Min.VP16ADs misc_signal 3519..4091 /label=EMCV IRES CDS 4095..5117 /label=hph polyA_signal 5126..5383 /label=PGK pA misc_signal 5596..5830 /label=PB 3' TR primer_bind complement(5849..5868) /label=T3 rep_origin complement(6273..6955) /direction=LEFT /label=ColE1 ori CDS complement(7053..7712) /label=AmpR ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac 661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt 721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc 781 cgtgaggcgt gcttgtcaat gaggtaagtg tcactgattt tgaactataa cgaccgcgtg 841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat 901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta 961 tagatatcgt cgacattgat tattgactag ctagttatta atagtaatca attacggggt 1021 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 1081 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 1141 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 1201 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 1261 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 1321 agtacatcta cgtattagtc atcgctatta ccatgatggt cgaggtgagc cccacgttct 1381 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt 1441 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg 1501 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg 1561 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc 1621 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg 1681 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc 1741 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt 1801 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg 1861 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc 1921 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg 1981 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag 2041 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc 2101 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg 2161 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg 2221 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg 2281 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt 2341 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg 2401 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga 2461 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc 2521 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt 2581 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct 2641 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca 2701 aagaattcct cgaggccacc atgtctagac tggacaagag caaagtcata aacggcgctc 2761 tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc 2821 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg 2881 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt 2941 catggcaaga ctttctgcgg aacaacgcca agtcattccg ctgtgctctc ctctcacatc 3001 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg 3061 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc 3121 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag 3181 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa 3241 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca 3301 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg 3361 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc 3421 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaagcg gccgctcgat 3481 aagcttgata tcgaattccg cccccccccc ctctccctcc ccccccccta acgttactgg 3541 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 3601 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 3661 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 3721 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 3781 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 3841 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 3901 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 3961 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa 4021 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 4081 atatggccac aaccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga 4141 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg 4201 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg 4261 gtttctacaa agatcgttat gttcatcggc actttgcatc ggccgcgctc ccgattccgg 4321 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac 4381 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg 4441 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat 4501 tcggaccgca aggaatcggt caatacacta cgtggcgtga tttcatatgc gcgattgctg 4561 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc 4621 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc 4681 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg 4741 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga 4801 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc 4861 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc 4921 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa 4981 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg 5041 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc 5101 gtccgagggc aaaggaatag tcgagaaatt gatgatctat taagcaataa agacgtccac 5161 taaaatggaa gtttttcctg tcatactttg ttaagaaggg tgagaacaga gtacctacat 5221 tttgaatgga aggattggag ctacgggggt gggggtgggg tgggattaga taaatgcctg 5281 ctctttactg aaggctcttt actattgctt tatgataatg tttcatagtt ggatatcata 5341 atttaaacaa gcaaaaccaa attaagggcc agctcattcc tccactcacg atctatagat 5401 ccactagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 5461 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 5521 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 5581 cgtgccagcg gatcctttgt tactttatag aagaaatttt gagtttttgt ttttttttaa 5641 taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat 5701 ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa 5761 acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt 5821 ctagggttaa agagctccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg 5881 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 5941 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 6001 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 6061 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 6121 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 6181 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 6241 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 6301 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 6361 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 6421 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 6481 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 6541 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 6601 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 6661 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 6721 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 6781 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 6841 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 6901 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 6961 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 7021 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 7081 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 7141 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 7201 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 7261 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 7321 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 7381 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 7441 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 7501 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 7561 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 7621 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 7681 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 7741 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 7801 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 7861 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 7921 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 7981 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac //