LOCUS Exported 6706 bp ds-DNA circular SYN 27-OCT-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6706) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..6706 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(135..441) /direction=LEFT /label=f1(-)ori primer_bind 622..643 /label=T7 misc_signal complement(656..968) /label=PB 5' TR misc_binding 1044..1295 /label=TREmod promoter 1298..1364 /label=CMV min promoter intron 1402..1974 /label=b-globin intron mat_peptide 2062..2088 /label=HA misc_signal 2135..2706 /label=EMCV IRES CDS 2710..3426 /label=Venus polyA_signal 3506..3778 /label=PGK pA polyA_signal 3833..4044 /label=bGH pA misc_signal 4264..4498 /label=PB 3' TR primer_bind complement(4517..4536) /label=T3 rep_origin complement(4941..5623) /direction=LEFT /label=ColE1 ori CDS complement(5721..6380) /label=AmpR ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac 661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt 721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc 781 cgtgaggcgt gcttgtcaat gaggtaagtg tcactgattt tgaactataa cgaccgcgtg 841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat 901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta 961 tagatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca 1021 tgatctatag atctggatcc gtcgagttta ctccctatca gtgatagaga acgtatgtcg 1081 agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc agtgatagag 1141 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta 1201 tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac gtatgtcgag 1261 tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct 1321 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattcga gctcggtacc 1381 cgggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct 1441 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga 1501 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc 1561 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac 1621 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag 1681 tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca 1741 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat 1801 tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg 1861 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag 1921 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg 1981 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcct cgagatctca 2041 cgcgtgacta gtgccatggc ctacccatac gacgtgccag actacgccta gcggccgctc 2101 gataagcttg atatcgaatt ccgcccctct ccctcccccc cccctaacgt tactggccga 2161 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg 2221 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg 2281 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt 2341 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac 2401 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca 2461 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg 2521 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg 2581 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac 2641 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg 2701 gccacaacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 2761 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 2821 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc 2881 tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac 2941 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 3001 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 3061 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 3121 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag 3181 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg 3241 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 3301 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 3361 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 3421 tacaagtaaa gcggccagct tatcgactcg ccgatagtgg aaaccgacgc cccagcactc 3481 gtccgagggc aaaggaatag tcgagaaatt gatgatctat taaacaataa agatgtccac 3541 atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac ctacattttg 3601 aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa tgcctgctct 3661 ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat atcataattt 3721 aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc tatagatcct 3781 aagcttatcg atgatctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc 3841 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 3901 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 3961 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 4021 ctggggatgc ggtgggctct atggcttctg aggcggaaag aacctgcagc ccaagcttgg 4081 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 4141 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 4201 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga 4261 tcctttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata aataaataaa 4321 cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt 4381 aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac acatgcgtca 4441 attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct agggttaaag 4501 agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca 4561 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 4621 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 4681 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 4741 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 4801 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 4861 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 4921 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 4981 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 5041 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 5101 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 5161 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 5221 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 5281 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 5341 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 5401 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 5461 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 5521 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 5581 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 5641 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 5701 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 5761 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 5821 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 5881 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 5941 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 6001 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 6061 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 6121 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 6181 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 6241 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 6301 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 6361 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 6421 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 6481 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 6541 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 6601 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 6661 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac //