LOCUS       Exported                6706 bp ds-DNA     circular SYN 27-OCT-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /label=f1(-)ori
     primer_bind     622..643
                     /label=T7
     misc_signal     complement(656..968)
                     /label=PB 5' TR
     misc_binding    1044..1295
                     /label=TREmod
     promoter        1298..1364
                     /label=CMV min promoter
     intron          1402..1974
                     /label=b-globin intron
     mat_peptide     2062..2088
                     /label=HA
     misc_signal     2135..2706
                     /label=EMCV IRES
     CDS             2710..3426
                     /label=Venus
     polyA_signal    3506..3778
                     /label=PGK pA
     polyA_signal    3833..4044
                     /label=bGH pA
     misc_signal     4264..4498
                     /label=PB 3' TR
     primer_bind     complement(4517..4536)
                     /label=T3
     rep_origin      complement(4941..5623)
                     /direction=LEFT
                     /label=ColE1 ori
     CDS             complement(5721..6380)
                     /label=AmpR
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gaggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca
     1021 tgatctatag atctggatcc gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     1081 agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc agtgatagag
     1141 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     1201 tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac gtatgtcgag
     1261 tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct
     1321 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattcga gctcggtacc
     1381 cgggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct
     1441 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
     1501 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc
     1561 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac
     1621 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag
     1681 tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca
     1741 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat
     1801 tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg
     1861 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag
     1921 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg
     1981 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcct cgagatctca
     2041 cgcgtgacta gtgccatggc ctacccatac gacgtgccag actacgccta gcggccgctc
     2101 gataagcttg atatcgaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
     2161 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     2221 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     2281 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     2341 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     2401 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     2461 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     2521 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     2581 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac
     2641 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     2701 gccacaacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2761 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2821 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
     2881 tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac
     2941 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     3001 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     3061 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     3121 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     3181 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     3241 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3301 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3361 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3421 tacaagtaaa gcggccagct tatcgactcg ccgatagtgg aaaccgacgc cccagcactc
     3481 gtccgagggc aaaggaatag tcgagaaatt gatgatctat taaacaataa agatgtccac
     3541 atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac ctacattttg
     3601 aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa tgcctgctct
     3661 ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat atcataattt
     3721 aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc tatagatcct
     3781 aagcttatcg atgatctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     3841 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3901 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3961 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4021 ctggggatgc ggtgggctct atggcttctg aggcggaaag aacctgcagc ccaagcttgg
     4081 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4141 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4201 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     4261 tcctttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata aataaataaa
     4321 cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt
     4381 aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac acatgcgtca
     4441 attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct agggttaaag
     4501 agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
     4561 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4621 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4681 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4741 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4801 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4861 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4921 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4981 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5041 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5101 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5161 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5221 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5281 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5341 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     5401 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5461 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5521 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5581 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5641 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5701 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5761 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5821 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5881 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5941 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6001 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6061 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6121 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6181 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6241 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6301 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6361 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6421 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6481 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6541 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6601 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6661 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
