LOCUS Exported 8096 bp ds-DNA circular SYN 26-SEP-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8096) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..8096 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(135..441) /direction=LEFT /label=f1(-)ori primer_bind 622..643 /label=T7 misc_signal complement(656..968) /label=PB 5' TR misc_binding 1067..1378 /label=TRE promoter 1380..1499 /label=CMV min promoter intron 1539..2111 /label=b-globin intron gene 2178..3476 /label=Esrrb misc_signal 3525..4096 /label=EMCV IRES CDS 4100..4816 /label=Venus polyA_signal 4896..5168 /label=PGK pA polyA_signal 5223..5434 /label=bGH pA misc_signal 5654..5888 /label=PB 3' TR primer_bind complement(5907..5926) /label=T3 rep_origin complement(6331..7013) /direction=LEFT /label=ColE1 ori CDS complement(7111..7770) /label=AmpR ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac 661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt 721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc 781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg 841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat 901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta 961 tagatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca 1021 tgatctatag atctggatcc gtcgacaacg gcaagcttac atcgagttta ccactcccta 1081 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt 1141 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac 1201 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag 1261 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt 1321 taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt 1381 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 1441 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc 1501 cgcgggactc agacacccgg gatcctgaga acttcagggt gagtttgggg acccttgatt 1561 gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg 1621 tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt 1681 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg 1741 taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt 1801 atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat 1861 tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa 1921 tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca 1981 ccctggtcat catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga 2041 ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct 2101 ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag 2161 aattcctcga gaccaccatg tcgtccgaag acaggcacct gggctctagt tgcggctcct 2221 tcatcaagac ggagccatcc agcccgtcct cgggcattga tgccctcagc caccacagcc 2281 ccagcggctc gtcggacgcc agtggtggct ttggcattgc cctgagcacc cacgccaacg 2341 gtctggactc gccgcctatg ttcgcaggtg cggggctggg aggcaacccg tgccgcaaga 2401 gctacgagga ctgtactagt ggtatcatgg aggactccgc catcaaatgc gagtacatgc 2461 ttaacgccat ccccaagcgc ctgtgcctcg tgtgcgggga cattgcctct ggctaccact 2521 acggagtggc ctcctgcgag gcttgcaagg cgttcttcaa gagaaccatt caaggcaaca 2581 tcgagtacaa ctgcccggcc accaatgaat gtgagatcac caaacggagg cgcaagtcct 2641 gtcaggcctg ccgattcatg aaatgcctca aagtggggat gctgaaggaa ggtgtgcgcc 2701 ttgaccgagt tcgaggaggc cgccagaagt acaagcgacg gctggattcg gagaacagcc 2761 cctacctgaa cctgccgatt tccccacctg ctaaaaagcc attgactaag atcgtctcga 2821 atctactagg ggttgagcag gacaagctgt atgctatgcc tcccaacgat atccccgagg 2881 gagatatcaa ggccctgacc actctctgtg aattggcaga tcgggagctt gtgttcctca 2941 tcaactgggc caagcacatc ccaggcttcc ccagtctgac acttggggac cagatgagcc 3001 tgctgcagag tgcctggatg gagattctca tcttgggcat cgtgtaccgc tcgctcccat 3061 acgatgacaa gctggcatac gccgaggact atatcatgga tgaggaacac tctcgcctgg 3121 tagggctgct ggacctttac cgagccatcc tgcagctggt gcgcaggtac aagaaactca 3181 aggtagagaa ggaagagttt atgatcctca aggccctggc cctcgccaac tcagattcga 3241 tgtacattga gaacctggag gcggtgcaga agctccagga cctgctgcac gaggcgctgc 3301 aggactatga gctgagtcag cgccacgagg agccgcggag ggccggcaag ctgctgctga 3361 cgctgcccct gctgaggcag acagccgcca aagccgtgca acacttctac agtgtgaaac 3421 tgcagggcaa ggtgcccatg cacaaactct tcctggagat gctggaggcc aaggtgtgat 3481 gcggccgctc gataagcttg atatcgaatt ccgcccctct ccctcccccc cccctaacgt 3541 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac 3601 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag 3661 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa 3721 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag 3781 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga 3841 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag 3901 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc 3961 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag 4021 gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga 4081 tgataatatg gccacaacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 4141 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 4201 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct 4261 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg 4321 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 4381 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 4441 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 4501 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac 4561 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga 4621 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 4681 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga 4741 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 4801 ggacgagctg tacaagtaaa gcggccagct tatcgactcg ccgatagtgg aaaccgacgc 4861 cccagcactc gtccgagggc aaaggaatag tcgagaaatt gatgatctat taaacaataa 4921 agatgtccac atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac 4981 ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa 5041 tgcctgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat 5101 atcataattt aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc 5161 tatagatcct aagcttatcg atgatctaga gctcgctgat cagcctcgac tgtgccttct 5221 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc 5281 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt 5341 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat 5401 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aacctgcagc 5461 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 5521 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 5581 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 5641 tgccagcgga tcctttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata 5701 aataaataaa cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat 5761 aataaaactt aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac 5821 acatgcgtca attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct 5881 agggttaaag agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta 5941 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 6001 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 6061 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 6121 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 6181 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 6241 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 6301 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 6361 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 6421 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 6481 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 6541 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 6601 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 6661 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 6721 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 6781 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 6841 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 6901 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 6961 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7021 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7081 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7141 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 7201 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 7261 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7321 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 7381 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 7441 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 7501 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 7561 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 7621 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 7681 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 7741 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 7801 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 7861 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 7921 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 7981 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8041 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac //