LOCUS       Exported                8096 bp ds-DNA     circular SYN 26-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /label=f1(-)ori
     primer_bind     622..643
                     /label=T7
     misc_signal     complement(656..968)
                     /label=PB 5' TR
     misc_binding    1067..1378
                     /label=TRE
     promoter        1380..1499
                     /label=CMV min promoter
     intron          1539..2111
                     /label=b-globin intron
     gene            2178..3476
                     /label=Esrrb
     misc_signal     3525..4096
                     /label=EMCV IRES
     CDS             4100..4816
                     /label=Venus
     polyA_signal    4896..5168
                     /label=PGK pA
     polyA_signal    5223..5434
                     /label=bGH pA
     misc_signal     5654..5888
                     /label=PB 3' TR
     primer_bind     complement(5907..5926)
                     /label=T3
     rep_origin      complement(6331..7013)
                     /direction=LEFT
                     /label=ColE1 ori
     CDS             complement(7111..7770)
                     /label=AmpR
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca
     1021 tgatctatag atctggatcc gtcgacaacg gcaagcttac atcgagttta ccactcccta
     1081 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     1141 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     1201 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     1261 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     1321 taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt
     1381 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     1441 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     1501 cgcgggactc agacacccgg gatcctgaga acttcagggt gagtttgggg acccttgatt
     1561 gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg
     1621 tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt
     1681 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg
     1741 taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt
     1801 atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat
     1861 tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa
     1921 tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca
     1981 ccctggtcat catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga
     2041 ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct
     2101 ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag
     2161 aattcctcga gaccaccatg tcgtccgaag acaggcacct gggctctagt tgcggctcct
     2221 tcatcaagac ggagccatcc agcccgtcct cgggcattga tgccctcagc caccacagcc
     2281 ccagcggctc gtcggacgcc agtggtggct ttggcattgc cctgagcacc cacgccaacg
     2341 gtctggactc gccgcctatg ttcgcaggtg cggggctggg aggcaacccg tgccgcaaga
     2401 gctacgagga ctgtactagt ggtatcatgg aggactccgc catcaaatgc gagtacatgc
     2461 ttaacgccat ccccaagcgc ctgtgcctcg tgtgcgggga cattgcctct ggctaccact
     2521 acggagtggc ctcctgcgag gcttgcaagg cgttcttcaa gagaaccatt caaggcaaca
     2581 tcgagtacaa ctgcccggcc accaatgaat gtgagatcac caaacggagg cgcaagtcct
     2641 gtcaggcctg ccgattcatg aaatgcctca aagtggggat gctgaaggaa ggtgtgcgcc
     2701 ttgaccgagt tcgaggaggc cgccagaagt acaagcgacg gctggattcg gagaacagcc
     2761 cctacctgaa cctgccgatt tccccacctg ctaaaaagcc attgactaag atcgtctcga
     2821 atctactagg ggttgagcag gacaagctgt atgctatgcc tcccaacgat atccccgagg
     2881 gagatatcaa ggccctgacc actctctgtg aattggcaga tcgggagctt gtgttcctca
     2941 tcaactgggc caagcacatc ccaggcttcc ccagtctgac acttggggac cagatgagcc
     3001 tgctgcagag tgcctggatg gagattctca tcttgggcat cgtgtaccgc tcgctcccat
     3061 acgatgacaa gctggcatac gccgaggact atatcatgga tgaggaacac tctcgcctgg
     3121 tagggctgct ggacctttac cgagccatcc tgcagctggt gcgcaggtac aagaaactca
     3181 aggtagagaa ggaagagttt atgatcctca aggccctggc cctcgccaac tcagattcga
     3241 tgtacattga gaacctggag gcggtgcaga agctccagga cctgctgcac gaggcgctgc
     3301 aggactatga gctgagtcag cgccacgagg agccgcggag ggccggcaag ctgctgctga
     3361 cgctgcccct gctgaggcag acagccgcca aagccgtgca acacttctac agtgtgaaac
     3421 tgcagggcaa ggtgcccatg cacaaactct tcctggagat gctggaggcc aaggtgtgat
     3481 gcggccgctc gataagcttg atatcgaatt ccgcccctct ccctcccccc cccctaacgt
     3541 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     3601 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     3661 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     3721 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     3781 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     3841 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     3901 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     3961 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     4021 gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     4081 tgataatatg gccacaacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4141 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4201 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
     4261 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
     4321 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4381 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4441 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4501 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
     4561 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     4621 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4681 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     4741 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4801 ggacgagctg tacaagtaaa gcggccagct tatcgactcg ccgatagtgg aaaccgacgc
     4861 cccagcactc gtccgagggc aaaggaatag tcgagaaatt gatgatctat taaacaataa
     4921 agatgtccac atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac
     4981 ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa
     5041 tgcctgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat
     5101 atcataattt aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc
     5161 tatagatcct aagcttatcg atgatctaga gctcgctgat cagcctcgac tgtgccttct
     5221 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5281 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5341 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     5401 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aacctgcagc
     5461 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5521 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5581 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5641 tgccagcgga tcctttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata
     5701 aataaataaa cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat
     5761 aataaaactt aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac
     5821 acatgcgtca attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct
     5881 agggttaaag agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta
     5941 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6001 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6061 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6121 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6181 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6241 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6301 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6361 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6421 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6481 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6541 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6601 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6661 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6721 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6781 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6841 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6901 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6961 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7021 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7081 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7141 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7201 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7261 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7321 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7381 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7441 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7501 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7561 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7621 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7681 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7741 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7801 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7861 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7921 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7981 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8041 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
