LOCUS       Exported                8051 bp ds-DNA     circular SYN 13-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /label=f1(-)ori
     primer_bind     622..643
                     /label=T7
     misc_signal     complement(656..968)
                     /label=PB 5' TR
     misc_signal     complement(656..801)
                     /label=PB 5' TR
                     /label=PB 5' TR(1)
     misc_binding    1044..1295
                     /label=TREmod
     promoter        1298..1364
                     /label=CMV min promoter
     intron          1402..1974
                     /label=b-globin intron
     mat_peptide     2059..2124
                     /label=3xFLAG
     gene            2146..3444
                     /label=Esrrb
     misc_signal     3498..4069
                     /label=EMCV IRES
     mat_peptide     4073..4450
                     /label=H2BJ
     CDS             4469..5122
                     /label=mKO2
     polyA_signal    5178..5389
                     /label=bGH pA
     misc_signal     5609..5843
                     /label=PB 3' TR
     primer_bind     complement(5862..5881)
                     /label=T3
     rep_origin      complement(6286..6968)
                     /direction=LEFT
                     /label=ColE1 ori
     CDS             complement(7066..7725)
                     /label=AmpR
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca
     1021 tgatctatag atctggatcc gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     1081 agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc agtgatagag
     1141 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     1201 tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac gtatgtcgag
     1261 tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct
     1321 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattcga gctcggtacc
     1381 cgggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct
     1441 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
     1501 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc
     1561 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac
     1621 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag
     1681 tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca
     1741 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat
     1801 tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg
     1861 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag
     1921 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg
     1981 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcct cgagatctca
     2041 cgcgtgacta gtgccatgga ctacaaggac cacgacggcg actacaagga ccacgacatc
     2101 gactacaagg acgacgacga caagggctcc ggagtcgaga ccaccatgtc gtccgaagac
     2161 aggcacctgg gctctagttg cggctccttc atcaagacgg agccatccag cccgtcctcg
     2221 ggcattgatg ccctcagcca ccacagcccc agcggctcgt cggacgccag tggtggcttt
     2281 ggcattgccc tgagcaccca cgccaacggt ctggactcgc cgcctatgtt cgcaggtgcg
     2341 gggctgggag gcaacccgtg ccgcaagagc tacgaggact gtactagtgg tatcatggag
     2401 gactccgcca tcaaatgcga gtacatgctt aacgccatcc ccaagcgcct gtgcctcgtg
     2461 tgcggggaca ttgcctctgg ctaccactac ggagtggcct cctgcgaggc ttgcaaggcg
     2521 ttcttcaaga gaaccattca aggcaacatc gagtacaact gcccggccac caatgaatgt
     2581 gagatcacca aacggaggcg caagtcctgt caggcctgcc gattcatgaa atgcctcaaa
     2641 gtggggatgc tgaaggaagg tgtgcgcctt gaccgagttc gaggaggccg ccagaagtac
     2701 aagcgacggc tggattcgga gaacagcccc tacctgaacc tgccgatttc cccacctgct
     2761 aaaaagccat tgactaagat cgtctcgaat ctactagggg ttgagcagga caagctgtat
     2821 gctatgcctc ccaacgatat ccccgaggga gatatcaagg ccctgaccac tctctgtgaa
     2881 ttggcagatc gggagcttgt gttcctcatc aactgggcca agcacatccc aggcttcccc
     2941 agtctgacac ttggggacca gatgagcctg ctgcagagtg cctggatgga gattctcatc
     3001 ttgggcatcg tgtaccgctc gctcccatac gatgacaagc tggcatacgc cgaggactat
     3061 atcatggatg aggaacactc tcgcctggta gggctgctgg acctttaccg agccatcctg
     3121 cagctggtgc gcaggtacaa gaaactcaag gtagagaagg aagagtttat gatcctcaag
     3181 gccctggccc tcgccaactc agattcgatg tacattgaga acctggaggc ggtgcagaag
     3241 ctccaggacc tgctgcacga ggcgctgcag gactatgagc tgagtcagcg ccacgaggag
     3301 ccgcggaggg ccggcaagct gctgctgacg ctgcccctgc tgaggcagac agccgccaaa
     3361 gccgtgcaac acttctacag tgtgaaactg cagggcaagg tgcccatgca caaactcttc
     3421 ctggagatgc tggaggccaa ggtgtctaga tgagcggccg ctcgataagc ttgatatcga
     3481 attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     3541 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     3601 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     3661 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     3721 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     3781 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     3841 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     3901 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     3961 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac
     4021 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgccaga
     4081 gccagcgaag tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca
     4141 gaagaaagac ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta
     4201 caaggttctg aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat
     4261 gaattcgttt gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca
     4321 ttacaacaag cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct
     4381 gcctggggag ttggccaagc acgccgtgtc cgagggtact aaggccgtca ccaagtacac
     4441 cagcgctaag ctcgagacta gtgccaccat ggtgagtgtg attaaaccag agatgaagat
     4501 gaggtactac atggacggct ccgtcaatgg gcatgagttc acaattgaag gtgaaggcac
     4561 aggcagacct tacgagggac atcaagagat gacactacgc gtcacaatgg ccgagggcgg
     4621 gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc tgttacggcc acagagtatt
     4681 tactaaatat ccagaagaga taccagacta tttcaaacaa gcatttcctg aaggcctgtc
     4741 atgggaaagg tcgttggagt tcgaagatgg tgggtccgct tcagtcagtg cgcatataag
     4801 ccttagagga aacaccttct accacaaatc caaatttact ggggttaact ttcctgccga
     4861 tggtcctatc atgcaaaacc aaagtgttga ttgggagcca tcaaccgaga aaattactgc
     4921 cagcgacgga gttctgaagg gtgatgttac gatgtaccta aaacttgaag gaggcggcaa
     4981 tcacaaatgc caaatgaaga ctacttacaa ggcggcaaaa gagattcttg aaatgccagg
     5041 agaccattac atcggccatc gcctcgtcag gaaaaccgaa ggcaacatta ctgagcaggt
     5101 agaagatgca gtagctcatt ccgctagctg aatcgatgat ctagagctcg ctgatcagcc
     5161 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5221 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5281 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5341 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5401 gaaagaacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5461 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5521 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     5581 tcgggaaacc tgtcgtgcca gcggatcctt tgttacttta tagaagaaat tttgagtttt
     5641 tgtttttttt taataaataa ataaacataa ataaattgtt tgttgaattt attattagta
     5701 tgtaagtgta aatataataa aacttaatat ctattcaaat taataaataa acctcgatat
     5761 acagaccgat aaaacacatg cgtcaatttt acgcatgatt atctttaacg tacgtcacaa
     5821 tatgattatc tttctagggt taaagagctc cagcttttgt tccctttagt gagggttaat
     5881 ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5941 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6001 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6061 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6121 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6181 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6241 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6301 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6361 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6421 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6481 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6541 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6601 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6661 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6721 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     6781 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6841 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6901 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6961 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7021 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7081 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7141 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7201 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7261 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7321 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7381 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7441 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7501 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7561 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7621 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7681 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7741 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7801 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7861 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7921 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7981 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8041 aaaagtgcca c
//
