LOCUS Exported 8021 bp ds-DNA circular SYN 29-NOV-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8021) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..8021 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(135..441) /direction=LEFT /label=f1(-)ori primer_bind 622..643 /label=T7 misc_signal complement(656..968) /label=PB 5' TR misc_binding 1044..1295 /label=TREmod promoter 1298..1364 /label=CMV min promoter intron 1402..1974 /label=b-globin intron gene 2041..3339 /label=Esrrb mat_peptide 3355..3420 /label=3xFLAG misc_signal 3468..4039 /label=EMCV IRES mat_peptide 4043..4420 /label=H2BJ CDS 4439..5092 /label=mKO2 polyA_signal 5148..5359 /label=bGH pA misc_signal 5579..5813 /label=PB 3' TR primer_bind complement(5832..5851) /label=T3 rep_origin complement(6256..6938) /direction=LEFT /label=ColE1 ori CDS complement(7036..7695) /label=AmpR ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac 661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt 721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc 781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg 841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat 901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta 961 tagatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca 1021 tgatctatag atctggatcc gtcgagttta ctccctatca gtgatagaga acgtatgtcg 1081 agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc agtgatagag 1141 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta 1201 tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac gtatgtcgag 1261 tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct 1321 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattcga gctcggtacc 1381 cgggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct 1441 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga 1501 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc 1561 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac 1621 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag 1681 tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca 1741 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat 1801 tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg 1861 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag 1921 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg 1981 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcct cgagaccacc 2041 atgtcgtccg aagacaggca cctgggctct agttgcggct ccttcatcaa gacggagcca 2101 tccagcccgt cctcgggcat tgatgccctc agccaccaca gccccagcgg ctcgtcggac 2161 gccagtggtg gctttggcat tgccctgagc acccacgcca acggtctgga ctcgccgcct 2221 atgttcgcag gtgcggggct gggaggcaac ccgtgccgca agagctacga ggactgtact 2281 agtggtatca tggaggactc cgccatcaaa tgcgagtaca tgcttaacgc catccccaag 2341 cgcctgtgcc tcgtgtgcgg ggacattgcc tctggctacc actacggagt ggcctcctgc 2401 gaggcttgca aggcgttctt caagagaacc attcaaggca acatcgagta caactgcccg 2461 gccaccaatg aatgtgagat caccaaacgg aggcgcaagt cctgtcaggc ctgccgattc 2521 atgaaatgcc tcaaagtggg gatgctgaag gaaggtgtgc gccttgaccg agttcgagga 2581 ggccgccaga agtacaagcg acggctggat tcggagaaca gcccctacct gaacctgccg 2641 atttccccac ctgctaaaaa gccattgact aagatcgtct cgaatctact aggggttgag 2701 caggacaagc tgtatgctat gcctcccaac gatatccccg agggagatat caaggccctg 2761 accactctct gtgaattggc agatcgggag cttgtgttcc tcatcaactg ggccaagcac 2821 atcccaggct tccccagtct gacacttggg gaccagatga gcctgctgca gagtgcctgg 2881 atggagattc tcatcttggg catcgtgtac cgctcgctcc catacgatga caagctggca 2941 tacgccgagg actatatcat ggatgaggaa cactctcgcc tggtagggct gctggacctt 3001 taccgagcca tcctgcagct ggtgcgcagg tacaagaaac tcaaggtaga gaaggaagag 3061 tttatgatcc tcaaggccct ggccctcgcc aactcagatt cgatgtacat tgagaacctg 3121 gaggcggtgc agaagctcca ggacctgctg cacgaggcgc tgcaggacta tgagctgagt 3181 cagcgccacg aggagccgcg gagggccggc aagctgctgc tgacgctgcc cctgctgagg 3241 cagacagccg ccaaagccgt gcaacacttc tacagtgtga aactgcaggg caaggtgccc 3301 atgcacaaac tcttcctgga gatgctggag gccaaggtgt ctagtggcag cggcgactac 3361 aaggaccacg acggcgacta caaggaccac gacatcgact acaaggacga cgacgacaag 3421 taggcggccg ctcgataagc ttgatatcga attccgcccc tctccctccc ccccccctaa 3481 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 3541 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 3601 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 3661 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 3721 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 3781 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 3841 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 3901 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc 3961 gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca 4021 cgatgataat atggccacaa ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa 4081 gggctccaag aaggcggtga ctaaggcgca gaagaaagac ggcaagaagc gcaagcgcag 4141 ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac 4201 cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg 4261 catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag 4321 ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc 4381 cgagggtact aaggccgtca ccaagtacac cagcgctaag ctcgagacta gtgccaccat 4441 ggtgagtgtg attaaaccag agatgaagat gaggtactac atggacggct ccgtcaatgg 4501 gcatgagttc acaattgaag gtgaaggcac aggcagacct tacgagggac atcaagagat 4561 gacactacgc gtcacaatgg ccgagggcgg gccaatgcct ttcgcgtttg acttagtgtc 4621 acacgtgttc tgttacggcc acagagtatt tactaaatat ccagaagaga taccagacta 4681 tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg 4741 tgggtccgct tcagtcagtg cgcatataag ccttagagga aacaccttct accacaaatc 4801 caaatttact ggggttaact ttcctgccga tggtcctatc atgcaaaacc aaagtgttga 4861 ttgggagcca tcaaccgaga aaattactgc cagcgacgga gttctgaagg gtgatgttac 4921 gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc caaatgaaga ctacttacaa 4981 ggcggcaaaa gagattcttg aaatgccagg agaccattac atcggccatc gcctcgtcag 5041 gaaaaccgaa ggcaacatta ctgagcaggt agaagatgca gtagctcatt ccgctagctg 5101 aatcgatgat ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 5161 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 5221 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 5281 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 5341 gatgcggtgg gctctatggc ttctgaggcg gaaagaacct gcagcccaag cttggcgtaa 5401 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 5461 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 5521 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatcctt 5581 tgttacttta tagaagaaat tttgagtttt tgtttttttt taataaataa ataaacataa 5641 ataaattgtt tgttgaattt attattagta tgtaagtgta aatataataa aacttaatat 5701 ctattcaaat taataaataa acctcgatat acagaccgat aaaacacatg cgtcaatttt 5761 acgcatgatt atctttaacg tacgtcacaa tatgattatc tttctagggt taaagagctc 5821 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct 5881 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 5941 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 6001 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 6061 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 6121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 6241 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 6301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 6361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 6421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 6481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 6541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 6601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 6661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 6721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 6781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 6841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 6901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 6961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 7021 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 7081 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 7141 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 7201 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 7261 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 7321 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 7381 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 7441 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 7501 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 7561 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 7621 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 7681 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 7741 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 7801 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 7861 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 7921 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 7981 acaaataggg gttccgcgca catttccccg aaaagtgcca c //