LOCUS       Exported                8021 bp ds-DNA     circular SYN 29-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /label=f1(-)ori
     primer_bind     622..643
                     /label=T7
     misc_signal     complement(656..968)
                     /label=PB 5' TR
     misc_binding    1044..1295
                     /label=TREmod
     promoter        1298..1364
                     /label=CMV min promoter
     intron          1402..1974
                     /label=b-globin intron
     gene            2041..3339
                     /label=Esrrb
     mat_peptide     3355..3420
                     /label=3xFLAG
     misc_signal     3468..4039
                     /label=EMCV IRES
     mat_peptide     4043..4420
                     /label=H2BJ
     CDS             4439..5092
                     /label=mKO2
     polyA_signal    5148..5359
                     /label=bGH pA
     misc_signal     5579..5813
                     /label=PB 3' TR
     primer_bind     complement(5832..5851)
                     /label=T3
     rep_origin      complement(6256..6938)
                     /direction=LEFT
                     /label=ColE1 ori
     CDS             complement(7036..7695)
                     /label=AmpR
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca
     1021 tgatctatag atctggatcc gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     1081 agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc agtgatagag
     1141 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     1201 tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac gtatgtcgag
     1261 tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct
     1321 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattcga gctcggtacc
     1381 cgggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct
     1441 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
     1501 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc
     1561 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac
     1621 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag
     1681 tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca
     1741 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat
     1801 tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg
     1861 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag
     1921 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg
     1981 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcct cgagaccacc
     2041 atgtcgtccg aagacaggca cctgggctct agttgcggct ccttcatcaa gacggagcca
     2101 tccagcccgt cctcgggcat tgatgccctc agccaccaca gccccagcgg ctcgtcggac
     2161 gccagtggtg gctttggcat tgccctgagc acccacgcca acggtctgga ctcgccgcct
     2221 atgttcgcag gtgcggggct gggaggcaac ccgtgccgca agagctacga ggactgtact
     2281 agtggtatca tggaggactc cgccatcaaa tgcgagtaca tgcttaacgc catccccaag
     2341 cgcctgtgcc tcgtgtgcgg ggacattgcc tctggctacc actacggagt ggcctcctgc
     2401 gaggcttgca aggcgttctt caagagaacc attcaaggca acatcgagta caactgcccg
     2461 gccaccaatg aatgtgagat caccaaacgg aggcgcaagt cctgtcaggc ctgccgattc
     2521 atgaaatgcc tcaaagtggg gatgctgaag gaaggtgtgc gccttgaccg agttcgagga
     2581 ggccgccaga agtacaagcg acggctggat tcggagaaca gcccctacct gaacctgccg
     2641 atttccccac ctgctaaaaa gccattgact aagatcgtct cgaatctact aggggttgag
     2701 caggacaagc tgtatgctat gcctcccaac gatatccccg agggagatat caaggccctg
     2761 accactctct gtgaattggc agatcgggag cttgtgttcc tcatcaactg ggccaagcac
     2821 atcccaggct tccccagtct gacacttggg gaccagatga gcctgctgca gagtgcctgg
     2881 atggagattc tcatcttggg catcgtgtac cgctcgctcc catacgatga caagctggca
     2941 tacgccgagg actatatcat ggatgaggaa cactctcgcc tggtagggct gctggacctt
     3001 taccgagcca tcctgcagct ggtgcgcagg tacaagaaac tcaaggtaga gaaggaagag
     3061 tttatgatcc tcaaggccct ggccctcgcc aactcagatt cgatgtacat tgagaacctg
     3121 gaggcggtgc agaagctcca ggacctgctg cacgaggcgc tgcaggacta tgagctgagt
     3181 cagcgccacg aggagccgcg gagggccggc aagctgctgc tgacgctgcc cctgctgagg
     3241 cagacagccg ccaaagccgt gcaacacttc tacagtgtga aactgcaggg caaggtgccc
     3301 atgcacaaac tcttcctgga gatgctggag gccaaggtgt ctagtggcag cggcgactac
     3361 aaggaccacg acggcgacta caaggaccac gacatcgact acaaggacga cgacgacaag
     3421 taggcggccg ctcgataagc ttgatatcga attccgcccc tctccctccc ccccccctaa
     3481 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     3541 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     3601 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     3661 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     3721 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     3781 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     3841 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     3901 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     3961 gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     4021 cgatgataat atggccacaa ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa
     4081 gggctccaag aaggcggtga ctaaggcgca gaagaaagac ggcaagaagc gcaagcgcag
     4141 ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac
     4201 cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg
     4261 catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag
     4321 ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc
     4381 cgagggtact aaggccgtca ccaagtacac cagcgctaag ctcgagacta gtgccaccat
     4441 ggtgagtgtg attaaaccag agatgaagat gaggtactac atggacggct ccgtcaatgg
     4501 gcatgagttc acaattgaag gtgaaggcac aggcagacct tacgagggac atcaagagat
     4561 gacactacgc gtcacaatgg ccgagggcgg gccaatgcct ttcgcgtttg acttagtgtc
     4621 acacgtgttc tgttacggcc acagagtatt tactaaatat ccagaagaga taccagacta
     4681 tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg
     4741 tgggtccgct tcagtcagtg cgcatataag ccttagagga aacaccttct accacaaatc
     4801 caaatttact ggggttaact ttcctgccga tggtcctatc atgcaaaacc aaagtgttga
     4861 ttgggagcca tcaaccgaga aaattactgc cagcgacgga gttctgaagg gtgatgttac
     4921 gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc caaatgaaga ctacttacaa
     4981 ggcggcaaaa gagattcttg aaatgccagg agaccattac atcggccatc gcctcgtcag
     5041 gaaaaccgaa ggcaacatta ctgagcaggt agaagatgca gtagctcatt ccgctagctg
     5101 aatcgatgat ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     5161 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5221 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5281 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     5341 gatgcggtgg gctctatggc ttctgaggcg gaaagaacct gcagcccaag cttggcgtaa
     5401 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5461 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5521 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatcctt
     5581 tgttacttta tagaagaaat tttgagtttt tgtttttttt taataaataa ataaacataa
     5641 ataaattgtt tgttgaattt attattagta tgtaagtgta aatataataa aacttaatat
     5701 ctattcaaat taataaataa acctcgatat acagaccgat aaaacacatg cgtcaatttt
     5761 acgcatgatt atctttaacg tacgtcacaa tatgattatc tttctagggt taaagagctc
     5821 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
     5881 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5941 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6001 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6061 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6241 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     6721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7021 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7081 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7141 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7201 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7261 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7321 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7381 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7441 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7501 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7561 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7621 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7681 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7741 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7801 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7861 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7921 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7981 acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
