LOCUS Exported 9619 bp ds-DNA circular SYN 19-FEB-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9619) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..9619 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(135..441) /direction=LEFT /label=f1(-)ori primer_bind 622..643 /label=T7 misc_signal complement(656..968) /label=PB 5' TR misc_feature 1048..4590 /label=Pou5f1P_3.5kb promoter 4619..4689 /label=UBC min promoter CDS 4707..5423 /label=EGFP misc_signal 5471..6043 /label=EMCV IRES CDS 6047..6643 /label=pac polyA_signal 6746..6957 /label=bGH pA misc_signal 7177..7411 /label=PB 3' TR primer_bind complement(7430..7449) /label=T3 rep_origin complement(7854..8536) /direction=LEFT /label=ColE1 ori CDS complement(8634..9293) /label=AmpR ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac 661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt 721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc 781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg 841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat 901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta 961 tagatatcgt catcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc 1021 cactcatgat ctatagatct cacgcgtagg gagacccagc ctggatgcta acacgagtga 1081 tttccctgct ctagtctagt gtcctccgtg agtccattta actgatcacc cagtctgtga 1141 ggaggtggct gaactcacag taagaaagct gtgggggtca acgcctattg tttgtttgtt 1201 ttgttttaga caaggtctcc tgctgaggct ggctcaagct ggcctggagg actcttgtgt 1261 ttaaggctgg ccttaaattc tctttaaaag aaaatcatgt gtatatatgt gtgtccatgt 1321 aactgcagat gaccacagca gccaagagat ttctgttctc ctagctgtaa accacccaat 1381 atgggtgcta ggaacagaat tttaaaaggg tcctctgaaa gagcaatgta cactcaaatg 1441 ctgagttctt tcccagcccc tagccttgga cctttgttct tatcacttcc aacgcccaag 1501 ggcaggcatt ataggtgtgg cattccgcat ctggcttccc aggatacctt ttcatgctgg 1561 tggaccatct ctggctgggg acgtgtgggc ttctctgctg tctttggttc tccagacaga 1621 actccgagac agatcttgac ttggttctaa aatacaggtg gtttgtggca agttaacgaa 1681 ttttagctca aatttggggt atttaagata ccatggtgac tcttttttgt ttgtttttct 1741 ttctttcttt ttttttttta aaaagattta tttattattg tacgtaagta cttcagacac 1801 cagaagaggg tgacagattt tttttttttt tcgagacagg atttctctgt gaagccctgg 1861 ctgtcctgga actcactctg tagaccaggc tggcctggaa ctcagaaatc cacctgcctc 1921 tgcctcccaa gtgctgggat taaagccatg agttgggacc gcacccggcc caaagtgact 1981 cttaaagggg gcagagtggc acatattttc aatcctagca ctcaggaagc aaaggctggt 2041 ggatctctgt gagttcaagg ccagcctggt ctacagagtg agttccaagc catctaaggc 2101 tatgtaggga acccttgaat caaacccaaa agttagtctg atgattttct aagacccagg 2161 aggcaagaaa ctggatcaga tgagccaaca ggtctgctgt cccatctcca gggccaccag 2221 gctcacagct cgggaccagg ctagggcaca tctgtttcaa gctagttcta agaagacttg 2281 ggacttcaga caaagttgct gttaaggact gtattatact ctaggcacgc ttagggctaa 2341 cctggttgca aagccagtca ctaggcagtt aaaggactca gaatatgtct cttgtcctgg 2401 ccagtgagtc accaaaagag aaatcacaat ccataagaca aggttggtat tgaatacaga 2461 caggactgct gggctgcagg catacttgaa ctgtggtgga gagtgctgtc taggccttag 2521 aggctggccc tgggaggaac tgggtgtggg gaggttgtag cccgaccctg cccctccccc 2581 cagggaggtt gagagttctg ggcagacggc agatgcataa caaaggtgca tgatagctct 2641 gccctggggg cagagaagat ggttggggag gggtccctct cgtcctagcc cttccttaat 2701 ctgctattga ggaagctttg tgaacttggc ggcttccaag tcgctgcctt tatttaggtc 2761 ttccaactaa cctatggcac tgttccacaa tgaatgtata gaaattggga ggtgagcatg 2821 acagagtgga ggaaacggaa gattcatgga gagggccaga gagatggccc ctcagccacc 2881 ctgggggatg acttggaccc atgtggtaga aggaggggac ttccacacat gtgctatgtg 2941 tagctgtgtg taggtacata cacaccctta aaataaaacg caattttttt ttcaaagtct 3001 cagggtgaat ttggtgaagt cgatgaagct gaggcaggag aattatcagg agttcaaggg 3061 cagcttgttt tatagagaaa ggttccatct ctacctgatg aagactacca tcaagagaca 3121 cccccgcccc ccagggcacc tagagccact gaccctagcc aacagctcag gcgggctggg 3181 cccaggctca gaactctgtc ctggctatgt acactgtggg gtgctctggg ctttttgagg 3241 ctgtgtgatt caccctgggg ccttcgttca gagcatggtg taggagcaga cagacaaaca 3301 ccatcccttg cagacaggca ctctgagggc tattctcttg caaagataac taagcaccag 3361 gccagtaatg ggatcctcag actgggccca gaaaaccact ctagggaagt tcagggtagg 3421 ctctctgcac cccctcctcc taatcccgtc tccttagtgt ctttccgcca gcacaggaat 3481 gggggagggg tgggtgacga ggatgaacac cggagtccct ggaggaaggg aagcagggta 3541 tctccatctg aggctctgtc tttgaggaga ggtggagagc tggggaagtc ttgtgtgagg 3601 ggattggggc tcaggagggg gttggggagc aggaagttgt ccccagggga gccatcctgg 3661 cccattcaag ggttgagtac ttgtttaggg ttagagctgc cccctctggg gaccaggatt 3721 gtccagccaa ggccattgtc ctgccccctt cccccagtcc ctcccaggcc cctttgaacc 3781 tgaagtcaga tatttcttct ctctacccac ctcccacccg ttgggtttct ccacccagga 3841 actaggctgg aagcctggga tgaggaggtg gggggaggga gaactgagaa tcttgaggaa 3901 agaggccccg gccttaactg tgaggggatg gagcctgggt gcaggtctta tgggggttgg 3961 ggggtggtta gtgtctaatc taccaacctg gacaacacaa gatggaatac tgtgctctga 4021 aaacgcagag ccagcacttc tctggggtct ctggggacat atctggttgg ggctcggggt 4081 cccatggtgt agagcctcta aactctggag gactggaggt gcaatggctg tcttgtcctg 4141 gccttggaca tgggctgaaa tactgggttc acccatatct aggactctag acgggtgggt 4201 aagcaagaac tgaggagtgg ccccagaaat aattggcaca cgaacattca atggatgttt 4261 taggctctcc agaggatggc tgagtgggct gtaaggacag gccgagaggg tgcagtgcca 4321 acaggctttg tggtgcgatg gggcatccga gcaactggtt tgtgaggtgt ccggtgaccc 4381 aaggcagggg tgagaggacc ttgaaggttg aaaatgaagg cctcctgggg tcccgtccta 4441 agggttgtcc tgtccagacg tccccaacct ccgtctggaa gacacaggca gatagcgctc 4501 gcctcagttt ctcccacccc cacagctctg ctcctccacc cacccagggg gcggggccag 4561 aggtcaaggc tagagggtgg gattggggag actagtgagg gggggcccgg tacccggggc 4621 ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag 4681 ccgggatttg aattcctcga gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg 4741 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 4801 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 4861 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 4921 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 4981 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 5041 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 5101 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 5161 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 5221 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 5281 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 5341 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 5401 tcggcatgga cgagctgtac aagtaagcgg ccgctcgata agcttgatat cgaattccgc 5461 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt 5521 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg 5581 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga 5641 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa 5701 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc 5761 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac 5821 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag 5881 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc 5941 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg 6001 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatga ccgagtacaa 6061 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc 6121 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg 6181 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 6241 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 6301 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 6361 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 6421 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 6481 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 6541 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 6601 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga 6661 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatgatct agagctcgct 6721 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 6781 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 6841 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 6901 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 6961 ctgaggcgga aagaacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg 7021 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 7081 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 7141 ctttccagtc gggaaacctg tcgtgccagc ggatcctttg ttactttata gaagaaattt 7201 tgagtttttg ttttttttta ataaataaat aaacataaat aaattgtttg ttgaatttat 7261 tattagtatg taagtgtaaa tataataaaa cttaatatct attcaaatta ataaataaac 7321 ctcgatatac agaccgataa aacacatgcg tcaattttac gcatgattat ctttaacgta 7381 cgtcacaata tgattatctt tctagggtta aagagctcca gcttttgttc cctttagtga 7441 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 7501 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 7561 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 7621 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 7681 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 7741 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 7801 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 7861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 7921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 7981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 8041 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 8101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 8161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 8221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 8281 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 8341 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 8401 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 8461 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 8521 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 8581 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 8641 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 8701 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 8761 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 8821 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 8881 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 8941 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 9001 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 9061 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 9121 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 9181 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 9241 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 9301 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 9361 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 9421 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 9481 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 9541 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 9601 tttccccgaa aagtgccac //