LOCUS       Exported                9619 bp ds-DNA     circular SYN 19-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /label=f1(-)ori
     primer_bind     622..643
                     /label=T7
     misc_signal     complement(656..968)
                     /label=PB 5' TR
     misc_feature    1048..4590
                     /label=Pou5f1P_3.5kb
     promoter        4619..4689
                     /label=UBC min promoter
     CDS             4707..5423
                     /label=EGFP
     misc_signal     5471..6043
                     /label=EMCV IRES
     CDS             6047..6643
                     /label=pac
     polyA_signal    6746..6957
                     /label=bGH pA
     misc_signal     7177..7411
                     /label=PB 3' TR
     primer_bind     complement(7430..7449)
                     /label=T3
     rep_origin      complement(7854..8536)
                     /direction=LEFT
                     /label=ColE1 ori
     CDS             complement(8634..9293)
                     /label=AmpR
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcgt catcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc
     1021 cactcatgat ctatagatct cacgcgtagg gagacccagc ctggatgcta acacgagtga
     1081 tttccctgct ctagtctagt gtcctccgtg agtccattta actgatcacc cagtctgtga
     1141 ggaggtggct gaactcacag taagaaagct gtgggggtca acgcctattg tttgtttgtt
     1201 ttgttttaga caaggtctcc tgctgaggct ggctcaagct ggcctggagg actcttgtgt
     1261 ttaaggctgg ccttaaattc tctttaaaag aaaatcatgt gtatatatgt gtgtccatgt
     1321 aactgcagat gaccacagca gccaagagat ttctgttctc ctagctgtaa accacccaat
     1381 atgggtgcta ggaacagaat tttaaaaggg tcctctgaaa gagcaatgta cactcaaatg
     1441 ctgagttctt tcccagcccc tagccttgga cctttgttct tatcacttcc aacgcccaag
     1501 ggcaggcatt ataggtgtgg cattccgcat ctggcttccc aggatacctt ttcatgctgg
     1561 tggaccatct ctggctgggg acgtgtgggc ttctctgctg tctttggttc tccagacaga
     1621 actccgagac agatcttgac ttggttctaa aatacaggtg gtttgtggca agttaacgaa
     1681 ttttagctca aatttggggt atttaagata ccatggtgac tcttttttgt ttgtttttct
     1741 ttctttcttt ttttttttta aaaagattta tttattattg tacgtaagta cttcagacac
     1801 cagaagaggg tgacagattt tttttttttt tcgagacagg atttctctgt gaagccctgg
     1861 ctgtcctgga actcactctg tagaccaggc tggcctggaa ctcagaaatc cacctgcctc
     1921 tgcctcccaa gtgctgggat taaagccatg agttgggacc gcacccggcc caaagtgact
     1981 cttaaagggg gcagagtggc acatattttc aatcctagca ctcaggaagc aaaggctggt
     2041 ggatctctgt gagttcaagg ccagcctggt ctacagagtg agttccaagc catctaaggc
     2101 tatgtaggga acccttgaat caaacccaaa agttagtctg atgattttct aagacccagg
     2161 aggcaagaaa ctggatcaga tgagccaaca ggtctgctgt cccatctcca gggccaccag
     2221 gctcacagct cgggaccagg ctagggcaca tctgtttcaa gctagttcta agaagacttg
     2281 ggacttcaga caaagttgct gttaaggact gtattatact ctaggcacgc ttagggctaa
     2341 cctggttgca aagccagtca ctaggcagtt aaaggactca gaatatgtct cttgtcctgg
     2401 ccagtgagtc accaaaagag aaatcacaat ccataagaca aggttggtat tgaatacaga
     2461 caggactgct gggctgcagg catacttgaa ctgtggtgga gagtgctgtc taggccttag
     2521 aggctggccc tgggaggaac tgggtgtggg gaggttgtag cccgaccctg cccctccccc
     2581 cagggaggtt gagagttctg ggcagacggc agatgcataa caaaggtgca tgatagctct
     2641 gccctggggg cagagaagat ggttggggag gggtccctct cgtcctagcc cttccttaat
     2701 ctgctattga ggaagctttg tgaacttggc ggcttccaag tcgctgcctt tatttaggtc
     2761 ttccaactaa cctatggcac tgttccacaa tgaatgtata gaaattggga ggtgagcatg
     2821 acagagtgga ggaaacggaa gattcatgga gagggccaga gagatggccc ctcagccacc
     2881 ctgggggatg acttggaccc atgtggtaga aggaggggac ttccacacat gtgctatgtg
     2941 tagctgtgtg taggtacata cacaccctta aaataaaacg caattttttt ttcaaagtct
     3001 cagggtgaat ttggtgaagt cgatgaagct gaggcaggag aattatcagg agttcaaggg
     3061 cagcttgttt tatagagaaa ggttccatct ctacctgatg aagactacca tcaagagaca
     3121 cccccgcccc ccagggcacc tagagccact gaccctagcc aacagctcag gcgggctggg
     3181 cccaggctca gaactctgtc ctggctatgt acactgtggg gtgctctggg ctttttgagg
     3241 ctgtgtgatt caccctgggg ccttcgttca gagcatggtg taggagcaga cagacaaaca
     3301 ccatcccttg cagacaggca ctctgagggc tattctcttg caaagataac taagcaccag
     3361 gccagtaatg ggatcctcag actgggccca gaaaaccact ctagggaagt tcagggtagg
     3421 ctctctgcac cccctcctcc taatcccgtc tccttagtgt ctttccgcca gcacaggaat
     3481 gggggagggg tgggtgacga ggatgaacac cggagtccct ggaggaaggg aagcagggta
     3541 tctccatctg aggctctgtc tttgaggaga ggtggagagc tggggaagtc ttgtgtgagg
     3601 ggattggggc tcaggagggg gttggggagc aggaagttgt ccccagggga gccatcctgg
     3661 cccattcaag ggttgagtac ttgtttaggg ttagagctgc cccctctggg gaccaggatt
     3721 gtccagccaa ggccattgtc ctgccccctt cccccagtcc ctcccaggcc cctttgaacc
     3781 tgaagtcaga tatttcttct ctctacccac ctcccacccg ttgggtttct ccacccagga
     3841 actaggctgg aagcctggga tgaggaggtg gggggaggga gaactgagaa tcttgaggaa
     3901 agaggccccg gccttaactg tgaggggatg gagcctgggt gcaggtctta tgggggttgg
     3961 ggggtggtta gtgtctaatc taccaacctg gacaacacaa gatggaatac tgtgctctga
     4021 aaacgcagag ccagcacttc tctggggtct ctggggacat atctggttgg ggctcggggt
     4081 cccatggtgt agagcctcta aactctggag gactggaggt gcaatggctg tcttgtcctg
     4141 gccttggaca tgggctgaaa tactgggttc acccatatct aggactctag acgggtgggt
     4201 aagcaagaac tgaggagtgg ccccagaaat aattggcaca cgaacattca atggatgttt
     4261 taggctctcc agaggatggc tgagtgggct gtaaggacag gccgagaggg tgcagtgcca
     4321 acaggctttg tggtgcgatg gggcatccga gcaactggtt tgtgaggtgt ccggtgaccc
     4381 aaggcagggg tgagaggacc ttgaaggttg aaaatgaagg cctcctgggg tcccgtccta
     4441 agggttgtcc tgtccagacg tccccaacct ccgtctggaa gacacaggca gatagcgctc
     4501 gcctcagttt ctcccacccc cacagctctg ctcctccacc cacccagggg gcggggccag
     4561 aggtcaaggc tagagggtgg gattggggag actagtgagg gggggcccgg tacccggggc
     4621 ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag
     4681 ccgggatttg aattcctcga gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     4741 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     4801 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     4861 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     4921 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4981 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     5041 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     5101 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     5161 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     5221 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     5281 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     5341 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5401 tcggcatgga cgagctgtac aagtaagcgg ccgctcgata agcttgatat cgaattccgc
     5461 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     5521 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     5581 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     5641 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     5701 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     5761 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     5821 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     5881 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     5941 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     6001 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatga ccgagtacaa
     6061 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     6121 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     6181 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6241 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6301 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     6361 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6421 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6481 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     6541 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6601 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     6661 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatgatct agagctcgct
     6721 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     6781 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     6841 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     6901 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     6961 ctgaggcgga aagaacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     7021 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7081 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7141 ctttccagtc gggaaacctg tcgtgccagc ggatcctttg ttactttata gaagaaattt
     7201 tgagtttttg ttttttttta ataaataaat aaacataaat aaattgtttg ttgaatttat
     7261 tattagtatg taagtgtaaa tataataaaa cttaatatct attcaaatta ataaataaac
     7321 ctcgatatac agaccgataa aacacatgcg tcaattttac gcatgattat ctttaacgta
     7381 cgtcacaata tgattatctt tctagggtta aagagctcca gcttttgttc cctttagtga
     7441 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7501 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7561 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7621 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7681 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7741 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7801 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8041 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8281 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8341 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8401 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8461 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8521 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8581 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8641 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8701 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8761 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8821 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8881 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8941 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9001 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9061 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9121 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9181 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9241 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9301 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9361 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9421 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9481 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9541 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9601 tttccccgaa aagtgccac
//
