LOCUS       Exported                4660 bp ds-DNA     circular SYN 19-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        45..115
                     /label=UBC min promoter
     CDS             188..1837
                     /label=luc2
     polyA_signal    complement(1876..2087)
                     /label=SV40 pA
     misc_feature    2112..2443
                     /label=Utf1-A'
     misc_feature    2240..2254
                     /label=OCT-SOX
     rep_origin      complement(2736..3418)
                     /direction=LEFT
                     /label=ColE1 ori
     CDS             complement(3545..4204)
                     /label=AmpR
     polyA_signal    4507..4555
                     /label=synthetic polyA
     misc_signal     complement(4568..4660)
                     /label=Transcription pause signal (hHBA2)
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tggggcggtg aacgccgatg
       61 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgaatt
      121 cctcgactct agaggatctg gcctcggcgg ccaagcttgg caatccggta ctgttggtaa
      181 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
      241 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
      301 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
      361 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
      421 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
      481 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
      541 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
      601 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
      661 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
      721 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
      781 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
      841 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
      901 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
      961 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     1021 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag
     1081 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc
     1141 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
     1201 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
     1261 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
     1321 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
     1381 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
     1441 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
     1501 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
     1561 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
     1621 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
     1681 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
     1741 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
     1801 tctcattaag gccaagaagg gcggcaagat cgccgtgtaa taattctaga gtcggggcgg
     1861 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     1921 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     1981 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2041 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     2101 cgataaggat ccttagcaaa gcccagctgc ccaactagaa ctttcaaagc tgaaattcca
     2161 aggtcctcag ggactagaga gtcagagtgg gggagggctt aggtgcaggt agaggaaagg
     2221 aagctgctgc cggcacttca ctagcataac aatgagggct catcctgagg ctccaattta
     2281 caaattctgg gcccatccgg cccttactgc taacccaaag actaagtctt ggctaaggga
     2341 agtcctgagg agtttatcca gatggtccca aggtcacaat tccccttctg aagacttgga
     2401 atagaacttc aggtactgac ttgtggtgtg gacctcctgc cagtcgaccg atgcccttga
     2461 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     2521 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
     2581 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2641 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2701 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2761 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2821 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2881 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2941 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3001 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3061 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3121 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3181 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3241 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3301 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3361 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3421 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3481 taaagtatat atgagtaaac ttggtctgac agcggccgca aatgctaaac cactgcagtg
     3541 gttaccagtg cttgatcagt gaggcaccga tctcagcgat ctgcctattt cgttcgtcca
     3601 tagtggcctg actccccgtc gtgtagatca ctacgattcg tgagggctta ccatcaggcc
     3661 ccagcgcagc aatgatgccg cgagagccgc gttcaccggc ccccgatttg tcagcaatga
     3721 accagccagc agggagggcc gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc
     3781 agtctatgag ctgctgtcgt gatgctagag taagaagttc gccagtgagt agtttccgaa
     3841 gagttgtggc cattgctact ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt
     3901 tcaactctgg ttcccagcgg tcaagccggg tcacatgatc acccatatta tgaagaaatg
     3961 cagtcagctc cttagggcct ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc
     4021 tcatggtaat ggcagcacta cacaattctc ttaccgtcat gccatccgta agatgctttt
     4081 ccgtgaccgg cgagtactca accaagtcgt tttgtgagta gtgtatacgg cgaccaagct
     4141 gctcttgccc ggcgtctata cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc
     4201 tcatcatcgg gaatcgttct tcggggcgga aagactcaag gatcttgccg ctattgagat
     4261 ccagttcgat atagcccact cttgcaccca gttgatcttc agcatctttt actttcacca
     4321 gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga atgagtgcga
     4381 cacgaaaatg ttggatgctc atactcgtcc tttttcaata ttattgaagc atttatcagg
     4441 gttactagta cgtctctcaa ggataagtaa gtaatattaa ggtacgggag gtattggaca
     4501 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
     4561 atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag
     4621 gctgtcccca gtgcaagtgc aggtgccaga acatttctct
//
