LOCUS Exported 4966 bp ds-DNA circular SYN 19-FEB-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4966) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Feb 22, 2019 from SnapGene Viewer 4.3.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..4966 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 45..115 /label=UBC min promoter CDS 188..1837 /label=luc2 polyA_signal complement(1876..2087) /label=SV40 pA misc_feature 2111..2748 /label=Klf2-D' rep_origin complement(3042..3724) /direction=LEFT /label=ColE1 ori CDS complement(3851..4510) /label=AmpR polyA_signal 4813..4861 /label=synthetic polyA misc_signal complement(4874..4966) /label=Transcription pause signal (hHBA2) ORIGIN 1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tggggcggtg aacgccgatg 61 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgaatt 121 cctcgactct agaggatctg gcctcggcgg ccaagcttgg caatccggta ctgttggtaa 181 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga 241 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg 301 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga 361 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat 421 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat 481 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat 541 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa 601 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta 661 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga 721 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag 781 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg 841 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct 901 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg 961 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca 1021 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag 1081 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc 1141 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca 1201 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa 1261 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac 1321 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat 1381 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct 1441 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct 1501 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct 1561 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg 1621 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat 1681 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt 1741 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat 1801 tctcattaag gccaagaagg gcggcaagat cgccgtgtaa taattctaga gtcggggcgg 1861 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag 1921 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac 1981 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt 2041 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat 2101 cgataaggat ctgcatccca agcaccaagc aggtgacaca cccatgtcat cccagtacta 2161 gggaagcagg caggaggatc ctgagttcaa aagtatccac agctatggtg agctatggct 2221 accctttgct acatgacttt tttttttcct aaagtttaaa gtcataacca acatgttcct 2281 aaaagtggtc aagcctcgct tgtccaggaa aaagagtttt gaaacatact cacaagcacc 2341 actgtcttgc tttaagctac agtagtagat cacaacaaac cagactgaac tgggctacga 2401 ccttgggaag aatctgcact taacctggct agtgcagttg acatgacaaa gacagggcct 2461 tcagtctaga gcctcacaca aaagaggcca ccaacagaag ccaggaggcc aatcaagccc 2521 agaatttttt tttattgtgt ttctagtttg caaagagtgt gactttgaaa tgcctaatct 2581 gcatcttttc cctaaaacca aaaaaacaaa caaaaccaac tcggaataac aacaacaaca 2641 aaaaagcttg tagaaagtgg tatggggtgt ggcagcctgt tcctccagct tcagcctccg 2701 gattgccagg aactggggat tgaagccgcc tttcttggtc ccctatccgt cgaccgatgc 2761 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg 2821 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct 2881 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 2941 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 3001 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 3061 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 3121 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 3181 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 3241 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 3301 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 3361 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 3421 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 3481 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 3541 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 3601 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 3661 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 3721 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 3781 tcaatctaaa gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact 3841 gcagtggtta ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt 3901 cgtccatagt ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat 3961 caggccccag cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag 4021 caatgaacca gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct 4081 ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt 4141 tccgaagagt tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg 4201 cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa 4261 gaaatgcagt cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt 4321 tgtcgctcat ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat 4381 gcttttccgt gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac 4441 caagctgctc ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga 4501 aagtgctcat catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat 4561 tgagatccag ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt 4621 tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga 4681 gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt 4741 atcagggtta ctagtacgtc tctcaaggat aagtaagtaa tattaaggta cgggaggtat 4801 tggacaggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt 4861 gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca 4921 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctct //