LOCUS       pESF140_pAC8RedH        9203 bp    DNA     circular UNA 22-FEB-2019
DEFINITION  .
ACCESSION   urn.local.nowak.7t-a58n122
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1359..1665
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="f1 origin"
                     /Transferred_Similarity="100.00%"
                     /label="f1 origin"
                     /note="Geneious type: origin of replication"
     promoter        2114..2142
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     gene            4040..5186
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="Ac_5_flank"
                     /Transferred_Similarity="100.00%"
                     /label="Ac_5_flank"
     gene            5197..5230
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="loxP"
                     /Transferred_Similarity="100.00%"
                     /label="loxP"
     promoter        6386..6486
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="PH prom"
                     /Transferred_Similarity="100.00%"
                     /label="PH prom"
     misc_feature    6490..6501
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    6504..6531
                     /Original_Bases="ATCTACCATGGACTGGAGCCACCCGCAGTTCGAAAAATCGG
                     TCATGTCCGGCCTGAACGACATCTTCGAGGCTCAGAAAATCGAATGGCACGAAGGTGG
                     AGGAGGTTCTGGAGGCGGTGGAAGTGGTGGCGGAGGA"
                     /label="ATCTACCATGGACTGGAGCCACCCGCAGTTCGAAAAATCGGTCATGTCCG
                     GCCTGAACGACATCTTCGAGGCTCAGAAAATCGAATGGCACGAAGGTGGAGGAGGTTC
                     TGGAGGCGGTGGAAGTGGTGGCGGAGGA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6511..6528
                     /created_by="User"
                     /label="His6"
     misc_feature    6535..6573
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    6583..9087
                     /created_by="User"
                     /label="hsDDB1dB"
     misc_feature    7769..7782
                     /Original_Bases="TCCACGAGCATGCCAGCATTGACTTACCAGGCATCAAAGGA
                     TTATGGCCACTGCGGTCTGACCCTAATCGTGAGACTGATGACACTTTGGTGCTCTCTT
                     TTGTGGGCCAGACAAGAGTTCTCATGTTAAATGGAGAGGAGGTAGAAGAAACCGAACT
                     GATGGGTTTCGTGGATGATCAGCAGACTTTCTTCTGTGGCAACGTGGCTCATCAGCAG
                     CTTATCCAGATCACTTCAGCATCGGTGAGGTTGGTCTCTCAAGAACCCAAAGCTCTGG
                     TCAGTGAATGGAAGGAGCCTCAGGCCAAGAACATCAGTGTGGCCTCCTGCAATAGCAG
                     CCAGGTGGTGGTGGCTGTAGGCAGGGCCCTCTACTATCTGCAGATCCATCCTCAGGAG
                     CTCCGGCAGATCAGCCACACAGAGATGGAACATGAAGTGGCTTGCTTGGACATCACCC
                     CATTAGGAGACAGCAATGGACTGTCCCCTCTTTGTGCCATTGGCCTCTGGACGGACAT
                     CTCGGCTCGTATCTTGAAGTTGCCTTCTTTTGAACTACTGCACAAGGAGATGCTGGGT
                     GGAGAGATCATTCCTCGCTCCATCCTGATGACCACCTTTGAGAGTAGCCACTACCTCC
                     TTTGTGCCTTGGGAGATGGAGCGCTTTTCTACTTTGGGCTCAACATTGAGACAGGTCT
                     GTTGAGTGACCGTAAGAAGGTGACTTTAGGCACCCAGCCCACCGTATTGAGGACTTTT
                     CGTTCTCTTTCTACTACCAACGTCTTTGCTTGCTCTGACCGCCCCACTGTCATCTATA
                     GCAGCAACCACAAATTGGTCTTCTCAAATGTCAACCTCAAGGAAGTGAACTACATGTG
                     TCCCCTCAATTCAGATGGCTATCCTGACAGCCTGGCACTGGCCAACAATAGCACCCTC
                     ACCATTGGCACCATCGAC"
                     /label="TCCACGAGCATGCCAGCATTGACTTACCAGGCATCAAAGGATTATGGCCA
                     CTGCGGTCTGACCCTAATCGTGAGACTGATGACACTTTGGTGCTCTCTTTTGTGGGCC
                     AGACAAGAGTTCTCATGTTAAATGGAGAGGAGGTAGAAGAAACCGAACTGATGGGTTT
                     CGTGGATGATCAGCAGACTTTCTTCTGTGGCAACGTGGCTCATCAGCAGCTTATCCAG
                     ATCACTTCAGCATCGGTGAGGTTGGTCTCTCAAGAACCCAAAGCTCTGGTCAGTGAAT
                     GGAAGGAGCCTCAGGCCAAGAACATCAGTGTGGCCTCCTGCAATAGCAGCCAGGTGGT
                     GGTGGCTGTAGGCAGGGCCCTCTACTATCTGCAGATCCATCCTCAGGAGCTCCGGCAG
                     ATCAGCCACACAGAGATGGAACATGAAGTGGCTTGCTTGGACATCACCCCATTAGGAG
                     ACAGCAATGGACTGTCCCCTCTTTGTGCCATTGGCCTCTGGACGGACATCTCGGCTCG
                     TATCTTGAAGTTGCCTTCTTTTGAACTACTGCACAAGGAGATGCTGGGTGGAGAGATC
                     ATTCCTCGCTCCATCCTGATGACCACCTTTGAGAGTAGCCACTACCTCCTTTGTGCCT
                     TGGGAGATGGAGCGCTTTTCTACTTTGGGCTCAACATTGAGACAGGTCTGTTGAGTGA
                     CCGTAAGAAGGTGACTTTAGGCACCCAGCCCACCGTATTGAGGACTTTTCGTTCTCTT
                     TCTACTACCAACGTCTTTGCTTGCTCTGACCGCCCCACTGTCATCTATAGCAGCAACC
                     ACAAATTGGTCTTCTCAAATGTCAACCTCAAGGAAGTGAACTACATGTGTCCCCTCAA
                     TTCAGATGGCTATCCTGACAGCCTGGCACTGGCCAACAATAGCACCCTCACCATTGGC
                     ACCATCGAC"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    9089..9109
                     /Original_Bases="GAGACGGCCGCTTAATT"
                     /label="GAGACGGCCGCTTAATT"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
       61 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
      121 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
      181 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
      241 actcgacgta aacacgttaa ataaagcttg gacatattta acatcgggcg tgttagcttt
      301 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
      361 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
      421 tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct
      481 aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa
      541 catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat
      601 cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc
      661 agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt
      721 actggtttcg ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt
      781 tctaatagct tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt
      841 cggcattggt ggagcgggcg gcaattcaga catcgatggt ggtggtggtg gtggaggcgc
      901 tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc gccggtataa tttgttctgg
      961 tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct gcacaacgga
     1021 aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat tcaatattat aattggaata
     1081 caaatcgtaa aaatctgcta taagcattgt aatttcgcta tcgtttaccg tgccgatatt
     1141 taacaaccgc tcaatgtaag caattgtatt gtaaagagat tgtctcaagc tcggatcgat
     1201 cccgcacgcc gataacaagc cttttcattt ttactacagc attgtagtgg cgagacactt
     1261 cgctgtcgtc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1321 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1381 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1441 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1501 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     1561 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     1621 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     1681 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     1741 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     1801 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     1861 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     1921 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     1981 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     2041 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2101 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2161 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2221 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2281 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2341 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2401 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2461 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2521 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2581 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2641 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2701 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2761 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2821 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2881 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2941 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3001 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     3061 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3121 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3181 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3241 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3301 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3361 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3421 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3481 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3541 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3601 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3661 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3721 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3781 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3841 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3901 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3961 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4021 ggccgattca ttaatgcaga acggctccgc ccactattaa tgaaattaaa aattccaatt
     4081 ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg cgtagaagga aagaaaaatg
     4141 tcgtcgacat gctgaacaac aagattaata tgcctccgtg tataaaaaaa atattgaacg
     4201 atttgaaaga aaacaatgta ccgcgcggcg gtatgtacag gaagaggttt atactaaact
     4261 gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa ccgatgttta atcaaggctc
     4321 tgacgcattt ctacaaccac gactccaagt gtgtgggtga agtcatgcat cttttaatca
     4381 aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat gaaaactgtc gacaagctct
     4441 gtccgtttgc tggcaactgc aagggtctca atcctatttg taattattga ataataaaac
     4501 aattataaat gctaaatttg ttttttatta acgatacaaa ccaaacgcaa caagaacatt
     4561 tgtagtatta tctataattg aaaacgcgta gttataatcg ctgaggtaat atttaaaatc
     4621 attttcaaat gattcacagt taatttgcga caatataatt ttattttcac ataaactaga
     4681 cgccttgtcg tcttcttctt cgtattcctt ctctttttca tttttctcct cataaaaatt
     4741 aacatagtta ttatcgtatc catatatgta tctatcgtat agagtaaatt ttttgttgtc
     4801 ataaatatat atgtcttttt taatggggtg tatagtaccg ctgcgcatag tttttctgta
     4861 atttacaaca gtgctatttt ctggtagttc ttcggagtgt gttgctttaa ttattaaatt
     4921 tatataatca atgaatttgg gatcgtcggt tttgtacaat atgttgccgg catagtacgc
     4981 agcttcttct agttcaatta caccattttt tagcagcacc ggattaacat aactttccaa
     5041 aatgttgtac gaaccgttaa acaaaaacag ttcacctccc ttttctatac tattgtctgc
     5101 gagcagttgt ttgttgttaa aaataacagc cattgtaatg agacgcacaa actaatatca
     5161 caaactggaa atgtctatca atatatagtt gctgatataa cttcgtatag catacattat
     5221 acgaagttat ctggttgaaa cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     5281 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     5341 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     5401 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     5461 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     5521 cccccatctc ccgctattac tcgagtcact gggagccgga gtggcgggcc tcggcgtgct
     5581 cgtactgctc caccacggtg tagtcctcgt tgtggttggt gatgtccagc ttggagtcca
     5641 cgtagtggtt gccgggcagc tgcacgggct tcttggcctt gtacacggtc ttgaagtcgc
     5701 aggtgtagtg gccgccgtcc ttcagcttca gggcgtggga gatctcgccc ttcagcacgc
     5761 cgtcctgggg gtacagcttc tcggtggagg gctcccagcc ggcagtcttc ttctgcatta
     5821 cggggccgtc ggcggggaag ttcacgccct tgaacttcac cttgtagatg aaggtgccgt
     5881 cctgcaggga ggagtcctgc tgcacctcca ccacgccgcc gtcctcgaag ttcatggagc
     5941 gctcccaggt gaagccctcg gggaaggaca gcttcatgta gtcggggatg tcggcggggt
     6001 gcttcacgta ggccttggag ccgtactgga actgggggga caggatgtcc caggcgaagg
     6061 gcagggggcc gcccttggtc acctgcagct tggcggtctg ggtgccctcg tagggcttgc
     6121 cctcgccctc gccctcgatc tcgaagtagt ggccgttcac ggagccctcc atgcgcacct
     6181 tgaactgcat gaactccttg atgacgtcct cggtgttgtc catggtagag tgattgtaaa
     6241 taaaatgtaa tttacagtat agtattttaa ttaatataca aatgatttga taataattct
     6301 tatttaacta taatatattg tgttgggttg aattaaaggt ccgtatacta gtatcgattc
     6361 gcgacctact ccggaatatt aatagagttg ctgatatcat ggagataatt aaaatgataa
     6421 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
     6481 tataaatacg gatctaccat gggcagcagc catcatcatc atcatcactc cgcggcccac
     6541 atagttatgg tagatgcgta caagcctact aaaggcggcc gcatgtcgta caactacgtg
     6601 gtaacggccc agaagcccac cgccgtgaac ggctgcgtga ccggacactt tacttcggcc
     6661 gaagacttaa acctgttgat tgccaaaaac acgagattag agatctatgt ggtcaccgcc
     6721 gaggggcttc ggcccgtcaa agaggtgggc atgtatggga agattgcggt catggagctt
     6781 ttcaggccca agggggagag caaggacctg ctgtttatct tgacagcgaa gtacaatgcc
     6841 tgcatcctgg agtataaaca gagtggcgag agcattgaca tcattacgcg agcccatggc
     6901 aatgtccagg accgcattgg ccgcccctca gagaccggca ttattggcat cattgaccct
     6961 gagtgccgga tgattggcct gcgtctctat gatggccttt tcaaggttat tccactagat
     7021 cgcgataata aagaactcaa ggccttcaac atccgcctgg aggagctgca tgtcattgat
     7081 gtcaagttcc tatatggttg ccaagcacct actatttgct ttgtctacca ggaccctcag
     7141 gggcggcacg taaaaaccta tgaggtgtct ctccgagaaa aggaattcaa taagggccct
     7201 tggaaacagg aaaatgtcga agctgaagct tccatggtga tcgcagtccc agagcccttt
     7261 gggggggcca tcatcattgg acaggagtca atcacctatc acaatggtga caaatacctg
     7321 gctattgccc ctcctatcat caagcaaagc acgattgtgt gccacaatcg agtggaccct
     7381 aatggctcaa gatacctgct gggagacatg gaaggccggc tcttcatgct gcttttggag
     7441 aaggaggaac agatggatgg caccgtcact ctcaaggatc tccgtgtaga actccttgga
     7501 gagacctcta ttgctgagtg cttgacatac cttgataatg gtgttgtgtt tgtcgggtct
     7561 cgcctgggtg actcccagct tgtgaagctc aacgttgaca gtaatgaaca aggctcctat
     7621 gtagtggcca tggaaacctt taccaactta ggacccattg tcgatatgtg cgtggtggac
     7681 ctggagaggc aggggcaggg gcagctggtc acttgctctg gggctttcaa ggaaggttct
     7741 ttgcggatca tccggaatgg aattggaaaa aaaaaaaaaa aagagatcca gaagctgcac
     7801 attcgcacag ttcccctcta tgaatctcca aggaagatct gctaccagga agtgtcccag
     7861 tgtttcgggg tcctctccag ccgcattgaa gtccaagaca cgagtggggg cacaacagcc
     7921 ttgaggccca gcgctagtac ccaggctctt tccagcagtg tcagctccag caagctgttc
     7981 tccagcagca ctgctcctca tgagacctcc tttggagaag aggtggaggt gcacaaccta
     8041 cttatcattg accaacacac ctttgaagtg cttcatgccc accagtttct gcagaatgaa
     8101 tatgccctca gcctggtctc ctgcaagctg ggcaaagacc ccaacactta cttcattgtg
     8161 ggcacagcaa tggtgtatcc tgaagaggca gagcccaagc agggtcgcat tgtggtcttt
     8221 cagtattcgg atggaaaact acagactgtg gctgaaaagg aagtgaaagg ggccgtgtac
     8281 tctatggtgg aatttaacgg gaagctgtta gccagcatca atagcacggt gcggctctat
     8341 gagtggacaa cagagaagga gctgcgcact gagtgcaacc actacaacaa catcatggcc
     8401 ctctacctga agaccaaggg cgacttcatc ctggtgggcg accttatgcg ctcagtgctg
     8461 ctgctcgcct acaagcccat ggaaggaaac tttgaagaga ttgctcgaga ctttaatccc
     8521 aactggatga gtgctgtgga aatcttggat gatgacaatt ttctgggggc cgaaaatgcc
     8581 tttaacttgt ttgtgtgtca aaaggacagc gctgccacca ctgacgagga gcggcagcac
     8641 ctccaggagg ttggtctttt ccacctgggc gagtttgtca atgtcttttg ccacggctct
     8701 ctggtaatgc agaatctggg tgagacttcc acccccacac aaggctcggt gctctttggc
     8761 acggtcaatg gcatgatagg gctggtgacc tcactgtcag agagctggta caacctcctg
     8821 ctggacatgc agaatcgtct caataaagtc atcaaaagtg tggggaagat cgagcactcc
     8881 ttctggagat cctttcacac tgagcggaag acagaaccag ccacaggttt catcgatggt
     8941 gacttgattg agagtttcct ggatatcagc cgccccaaga tgcaggaggt ggtggcaaac
     9001 ctacagtatg acgatggcag cggtatgaag cgagaggcta ctgcagacga cctcatcaag
     9061 gttgtggagg agctaactcg gatccattag ggtacccccg gacggccgca attgatccgg
     9121 gttattagta catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt
     9181 gtacgtattt taataattca tta
//
