LOCUS       pAJ75_hsCRBN_in_        8098 bp    DNA     circular UNA 22-FEB-2019
DEFINITION  .
ACCESSION   urn.local.nowak.34-a58j5h5
VERSION     urn.local.nowak.34-a58j5h5
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1359..1665
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="f1 origin"
                     /Transferred_Similarity="100.00%"
                     /label="f1 origin"
                     /note="Geneious type: origin of replication"
     promoter        2114..2142
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     gene            4040..5186
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="Ac_5_flank"
                     /Transferred_Similarity="100.00%"
                     /label="Ac_5_flank"
     gene            5197..5230
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="loxP"
                     /Transferred_Similarity="100.00%"
                     /label="loxP"
     promoter        6386..6486
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="PH prom"
                     /Transferred_Similarity="100.00%"
                     /label="PH prom"
     misc_feature    6511..6528
                     /created_by="User"
                     /label="His6"
     source          <6529..>7899
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome=3
                     /map="3p26.2"
                     /label="source Homo sapiens"
     gene            <6529..>7899
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /note="cereblon"
                     /db_xref="GeneID:51185"
                     /db_xref="HGNC:HGNC:30185"
                     /db_xref="MIM:609262"
                     /label="CRBN gene"
     exon            <6529..6640
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /inference="alignment:Splign:1.39.8"
                     /label="CRBN exon"
     misc_feature    6541..6558
                     /created_by="User"
                     /label="TEV site"
     CDS             6571..7899
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein x 0001"
                     /codon_start=1
                     /product="protein cereblon isoform 2"
                     /protein_id="NP_001166953.1"
                     /db_xref="GI:291045198"
                     /db_xref="CCDS:CCDS54547.1"
                     /db_xref="GeneID:51185"
                     /db_xref="HGNC:HGNC:30185"
                     /db_xref="MIM:609262"
                     /translation="MAGEGDQQDAAHNMGNHLPLLPESEEEDEMEVEDQDSKEAKKPN
                     IINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFH
                     PQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIG
                     RQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPSKPVSREDQC
                     SYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPSNP
                     IDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKN
                     EIFSLSLCGPMAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKI
                     CASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL"
                     /label="CRBN CDS"
     misc_feature    6571..7899
                     /Original_Bases="TAAACTATCTGCACGG"
                     /label="TAAACTATCTGCACGG"
                     /note="Geneious type: Editing History Replacement"
     exon            6641..6744
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /inference="alignment:Splign:1.39.8"
                     /label="CRBN exon"
     exon            6745..6947
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /inference="alignment:Splign:1.39.8"
                     /label="CRBN exon"
     exon            6948..7097
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /inference="alignment:Splign:1.39.8"
                     /label="CRBN exon"
     exon            7098..7257
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /inference="alignment:Splign:1.39.8"
                     /label="CRBN exon"
     exon            7258..7320
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /inference="alignment:Splign:1.39.8"
                     /label="CRBN exon"
     exon            7321..7405
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /inference="alignment:Splign:1.39.8"
                     /label="CRBN exon"
     exon            7406..7521
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /inference="alignment:Splign:1.39.8"
                     /label="CRBN exon"
     exon            7522..7586
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /inference="alignment:Splign:1.39.8"
                     /label="CRBN exon"
     misc_feature    7585..7896
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (Q96SW2.1);
                     Region: Thalidomide-binding"
                     /label="CRBN misc"
     exon            7587..7718
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /inference="alignment:Splign:1.39.8"
                     /label="CRBN exon"
     exon            7719..>7899
                     /gene="CRBN"
                     /gene_synonym="MRT2; MRT2A"
                     /inference="alignment:Splign:1.39.8"
                     /label="CRBN exon"
     gene            7936..1271
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="Ac_3_flank"
                     /Transferred_Similarity="100.00%"
                     /label="Ac_3_flank"
ORIGIN      
        1 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
       61 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
      121 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
      181 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
      241 actcgacgta aacacgttaa ataaagcttg gacatattta acatcgggcg tgttagcttt
      301 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
      361 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
      421 tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct
      481 aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa
      541 catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat
      601 cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc
      661 agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt
      721 actggtttcg ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt
      781 tctaatagct tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt
      841 cggcattggt ggagcgggcg gcaattcaga catcgatggt ggtggtggtg gtggaggcgc
      901 tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc gccggtataa tttgttctgg
      961 tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct gcacaacgga
     1021 aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat tcaatattat aattggaata
     1081 caaatcgtaa aaatctgcta taagcattgt aatttcgcta tcgtttaccg tgccgatatt
     1141 taacaaccgc tcaatgtaag caattgtatt gtaaagagat tgtctcaagc tcggatcgat
     1201 cccgcacgcc gataacaagc cttttcattt ttactacagc attgtagtgg cgagacactt
     1261 cgctgtcgtc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1321 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1381 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1441 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1501 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     1561 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     1621 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     1681 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     1741 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     1801 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     1861 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     1921 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     1981 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     2041 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2101 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2161 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2221 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2281 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2341 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2401 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2461 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2521 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2581 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2641 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2701 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2761 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2821 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2881 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2941 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3001 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     3061 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3121 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3181 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3241 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3301 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3361 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3421 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3481 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3541 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3601 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3661 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3721 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3781 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3841 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3901 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3961 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4021 ggccgattca ttaatgcaga acggctccgc ccactattaa tgaaattaaa aattccaatt
     4081 ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg cgtagaagga aagaaaaatg
     4141 tcgtcgacat gctgaacaac aagattaata tgcctccgtg tataaaaaaa atattgaacg
     4201 atttgaaaga aaacaatgta ccgcgcggcg gtatgtacag gaagaggttt atactaaact
     4261 gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa ccgatgttta atcaaggctc
     4321 tgacgcattt ctacaaccac gactccaagt gtgtgggtga agtcatgcat cttttaatca
     4381 aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat gaaaactgtc gacaagctct
     4441 gtccgtttgc tggcaactgc aagggtctca atcctatttg taattattga ataataaaac
     4501 aattataaat gctaaatttg ttttttatta acgatacaaa ccaaacgcaa caagaacatt
     4561 tgtagtatta tctataattg aaaacgcgta gttataatcg ctgaggtaat atttaaaatc
     4621 attttcaaat gattcacagt taatttgcga caatataatt ttattttcac ataaactaga
     4681 cgccttgtcg tcttcttctt cgtattcctt ctctttttca tttttctcct cataaaaatt
     4741 aacatagtta ttatcgtatc catatatgta tctatcgtat agagtaaatt ttttgttgtc
     4801 ataaatatat atgtcttttt taatggggtg tatagtaccg ctgcgcatag tttttctgta
     4861 atttacaaca gtgctatttt ctggtagttc ttcggagtgt gttgctttaa ttattaaatt
     4921 tatataatca atgaatttgg gatcgtcggt tttgtacaat atgttgccgg catagtacgc
     4981 agcttcttct agttcaatta caccattttt tagcagcacc ggattaacat aactttccaa
     5041 aatgttgtac gaaccgttaa acaaaaacag ttcacctccc ttttctatac tattgtctgc
     5101 gagcagttgt ttgttgttaa aaataacagc cattgtaatg agacgcacaa actaatatca
     5161 caaactggaa atgtctatca atatatagtt gctgatataa cttcgtatag catacattat
     5221 acgaagttat ctggttgaaa cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     5281 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     5341 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     5401 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     5461 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     5521 cccccatctc ccgctattac tcgagtcact gggagccgga gtggcgggcc tcggcgtgct
     5581 cgtactgctc caccacggtg tagtcctcgt tgtggttggt gatgtccagc ttggagtcca
     5641 cgtagtggtt gccgggcagc tgcacgggct tcttggcctt gtacacggtc ttgaagtcgc
     5701 aggtgtagtg gccgccgtcc ttcagcttca gggcgtggga gatctcgccc ttcagcacgc
     5761 cgtcctgggg gtacagcttc tcggtggagg gctcccagcc ggcagtcttc ttctgcatta
     5821 cggggccgtc ggcggggaag ttcacgccct tgaacttcac cttgtagatg aaggtgccgt
     5881 cctgcaggga ggagtcctgc tgcacctcca ccacgccgcc gtcctcgaag ttcatggagc
     5941 gctcccaggt gaagccctcg gggaaggaca gcttcatgta gtcggggatg tcggcggggt
     6001 gcttcacgta ggccttggag ccgtactgga actgggggga caggatgtcc caggcgaagg
     6061 gcagggggcc gcccttggtc acctgcagct tggcggtctg ggtgccctcg tagggcttgc
     6121 cctcgccctc gccctcgatc tcgaagtagt ggccgttcac ggagccctcc atgcgcacct
     6181 tgaactgcat gaactccttg atgacgtcct cggtgttgtc catggtagag tgattgtaaa
     6241 taaaatgtaa tttacagtat agtattttaa ttaatataca aatgatttga taataattct
     6301 tatttaacta taatatattg tgttgggttg aattaaaggt ccgtatacta gtatcgattc
     6361 gcgacctact ccggaatatt aatagagttg ctgatatcat ggagataatt aaaatgataa
     6421 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
     6481 tataaatacg gatctaccat gggcagcagc catcatcatc atcatcactc cgcggtcgac
     6541 gagaacctct acttccaggg aggcggccgc atggccggcg aaggagatca gcaggacgct
     6601 gcgcacaaca tgggcaacca cctgccgctc ctgcctgcag agagtgagga agaagatgaa
     6661 atggaagttg aagaccagga tagtaaagaa gccaaaaaac caaacatcat aaattttgac
     6721 accagtctgc cgacatcaca tacataccta ggtgctgata tggaagaatt tcatggcagg
     6781 actttgcacg atgacgacag ctgtcaggtg attccagttc ttccacaagt gatgatgatc
     6841 ctgattcccg gacagacatt acctcttcag ctttttcacc ctcaagaagt cagtatggtg
     6901 cggaatttaa ttcagaaaga tagaaccttt gctgttcttg catacagcaa tgtacaggaa
     6961 agggaagcac agtttggaac aacagcagag atatatgcct atcgagaaga acaggatttt
     7021 ggaattgaga tagtgaaagt gaaagcaatt ggaagacaaa ggttcaaagt ccttgagcta
     7081 agaacacagt cagatggaat ccagcaagct aaagtgcaaa ttcttcccga atgtgtgttg
     7141 ccttcaacca tgtctgcagt tcaattagaa tccctcaata agtgccagat atttccttca
     7201 aaacctgtct caagagaaga ccaatgttca tataaatggt ggcagaaata ccagaagaga
     7261 aagtttcatt gtgcaaatct aacttcatgg cctcgctggc tgtattcctt atatgatgct
     7321 gagaccttaa tggacagaat caagaaacag ctacgtgaat gggatgaaaa tctaaaagat
     7381 gattctcttc cttcaaatcc aatagatttt tcttacagag tagctgcttg tcttcctatt
     7441 gatgatgtat tgagaattca gctccttaaa attggcagtg ctatccagcg acttcgctgt
     7501 gaattagaca ttatgaataa atgtacttcc ctttgctgta aacaatgtca agaaacagaa
     7561 ataacaacca aaaatgaaat attcagttta tccttatgtg ggccgatggc agcttatgtg
     7621 aatcctcatg gatatgtgca tgagacactt actgtgtata aggcttgcaa cttgaatctg
     7681 ataggccggc cttctacaga acacagctgg tttcctgggt atgcctggac tgttgcccag
     7741 tgtaagatct gtgcaagcca tattggatgg aagtttacgg ccaccaaaaa agacatgtca
     7801 cctcaaaaat tttggggctt aacgcgatct gctctgttgc ccacgatccc agacactgaa
     7861 gatgaaataa gtccagacaa agtaatactt tgcttgtaag gtacccccgg acggccgctt
     7921 aattaattga tccgggttat tagtacattt attaagcgct agattctgtg cgttgttgat
     7981 ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct ttagggtggt
     8041 atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta aatattaa
//
