LOCUS Exported 6211 bp ds-DNA circular SYN 27-SEP-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6211) AUTHORS SILS-BCB TITLE Direct Submission JOURNAL Exported Sunday, Feb 24, 2019 from SnapGene 4.3.0 https://www.snapgene.com FEATURES Location/Qualifiers source 1..6211 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 53..81 /label=TrcDown misc_feature 53..58 /label=TTTACA (-35) misc_feature 76..81 /label=TATAAT (-10) misc_feature 114..118 /label=RBS (CAGGA) CDS 129..787 /label=OmpA CDS 793..1500 /codon_start=1 /product="bright monomeric yellow-green fluorescent protein derived from LanYFP (Shaner et al., 2013)" /label=mNeonGreen /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ KAFTDVMGMDELYK" misc_feature 829..831 /label=L>CTG misc_feature 832..834 /label=P>CCG misc_feature 838..840 /label=T>ACC misc_feature 847..849 /label=L>CTG misc_feature 910..912 /label=P>CCG misc_feature 931..933 /label=L>CTG misc_feature 958..960 /label=L>CTG misc_feature 970..972 /label=P>CCG misc_feature 985..987 /label=P>CCG misc_feature 994..996 /label=G>GGC misc_feature 1018..1020 /label=P>CCG misc_feature 1024..1026 /label=P>CCG misc_feature 1036..1038 /label=S>TCT misc_feature 1039..1041 /label=P>CCG misc_RNA 1053 /label=NcoI delete C>G Ala site misc_feature 1069..1071 /label=G>GGC misc_feature 1087..1089 /label=T>ACC misc_feature 1114..1116 /label=L>CTG misc_feature 1144..1146 /label=G>GGC misc_feature 1159..1161 /label=G>GGC misc_feature 1177..1179 /label=G>GGC misc_feature 1189..1191 /label=P>CCG misc_feature 1201..1203 /label=P>CCG misc_feature 1216..1218 /label=S>TCT misc_feature 1240..1242 /label=R>CGU misc_feature 1243..1245 /label=S>TCT misc_feature 1258..1260 /label=P>CCG misc_feature 1279..1281 /label=S>AGC misc_feature 1294..1296 /label=S>AGC misc_feature 1306..1308 /label=G>GGC misc_feature 1360..1362 /label=P>CCG misc_feature 1417..1419 /label=L>CTG misc_feature 1438..1440 /label=L>CTG CDS 1505..2216 /label=mCherry misc_feature 1538..1539 /label=ALA (GCC-GCT) CDS 2741..3599 /label=AmpR CDS 5073..6030 /label=LacIq ORIGIN 1 gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgtttacaat 61 taatcatccg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 121 cagaccatgg caaaaaagac agctatcgcg attgcagtgg cactggctgg tttcgctacc 181 gtagcgcagg ccgctccgaa agataacacc tggtacactg gtgctaaact gggctggtcc 241 cagtaccatg acactggcct catcggccaa cgcttggaga tctgccagaa cgtttgctac 301 tacctcggga cgctcaatgg tccgacccat gaaaaccaac tgggcgctgg tgcttttggt 361 ggttaccagg ttaacccgta tgttggcttt gaaatgggtt acgactggtt aggtcgtatg 421 ccgtacaaag gcagcgttga aaacggtgca tacaaagctc agggcgttca actgaccgct 481 aaactgggtt acccaatcac tgacgacctg gacatctaca ctcgtctggg tggcatggta 541 tggcgtgcag acactaaatc caacgtttat ggtaaaaacc acgacaccgg cgtttctccg 601 gtcttcgctg gcggtgttga gtacgcgatc actcctgaaa tcgctacccg tctggaatac 661 cagtggacca acaacatcgg tgacgcaggc cagatcggcc agcgtccgga caacggcatg 721 ctgagcctgg gtgtttccta ccgtttcggt cagggcgaag cagctccact tgaggatccc 781 cccgctgaat tgatggtgag caagggcgag gaggataaca tggcctctct cccagcgaca 841 catgagttac acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc 901 accggcaatc caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc 961 cagttctccc cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc 1021 taccctgacg ggatgtcgcc tttccaggcc gcgatggtag atggctccgg ataccaagtc 1081 catcgcacaa tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac 1141 gagggaagcc acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt 1201 cctgtgatga ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa gacttacccc 1261 aacgacaaaa ccatcatcag tacctttaag tggagttaca ccactggaaa tggcaagcgc 1321 taccggagca ctgcgcggac cacctacacc tttgccaagc caatggcggc taactatctg 1381 aagaaccagc cgatgtacgt gttccgtaag acggagctca agcactccaa gaccgagctc 1441 aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg gcatggacga gctgtacaag 1501 gaattcatgg tgagcaaggg cgaggaggat aacatggcta tcatcaagga gttcatgcgc 1561 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 1621 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc 1681 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg 1741 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg 1801 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg 1861 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 1921 cccgtaatgc agaagaagac tatgggctgg gaggcctcct ccgagcggat gtaccccgag 1981 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac 2041 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac 2101 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag 2161 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagct 2221 tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag 2281 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc 2341 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc 2401 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 2461 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag 2521 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa 2581 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac 2641 aaactctttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 2701 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 2761 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 2821 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 2881 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 2941 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga 3001 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 3061 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 3121 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 3181 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 3241 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctacagcaa tggcaacaac 3301 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 3361 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 3421 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 3481 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 3541 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 3601 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 3661 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 3721 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 3781 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 3841 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 3901 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 3961 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 4021 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 4081 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 4141 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 4201 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 4261 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 4321 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 4381 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 4441 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 4501 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt 4561 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg 4621 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg 4681 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg 4741 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 4801 ccgtcatcac cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat 4861 gcatttacgt tgacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc 4921 cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag 4981 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 5041 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 5101 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 5161 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 5221 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 5281 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 5341 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 5401 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 5461 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 5521 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 5581 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 5641 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 5701 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 5761 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 5821 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 5881 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 5941 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 6001 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagcgc 6061 gaattgatct ggtttgacag cttatcatcg actgcacggt gcaccaatgc ttctggcgtc 6121 aggcagccat cggaagctgt ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg 6181 tcgctcaagg cgcactcccg ttctggataa t //