LOCUS Exported 12236 bp ds-DNA circular SYN 25-FEB-2019 DEFINITION Clone#4 sequence correct WTC11 iPSCs red after nucleofection. ACCESSION . VERSION . KEYWORDS J36 CLYBL-TO-hNGN2-BSD-mApple SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12236) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, Feb 25, 2019 from SnapGene 3.3.4 http://www.snapgene.com COMMENT This file is created by Vector NTI http://www.invitrogen.com/ FEATURES Location/Qualifiers source 1..12236 /organism="synthetic DNA construct" /mol_type="other DNA" source 1376..2045 /organism="synthetic DNA construct" /mol_type="other DNA" /note="color: #ffffff" source 1376..2045 /organism="Cloning vector pNEBR-X1Hygro" /mol_type="other DNA" /note="color: #ffffff" source 1376..2045 /organism="Cloning vector pNEBR-X1Hygro" /mol_type="other DNA" /note="color: #ffffff" source 7010..8414 /label=Donor sequence /note="color: #fffe11" source 8415..8957 /label=Donor sequence /note="color: #fffe11" misc_recomb complement(413..568) /label=AAVS1 /label=AAV 3' Arm /note="residual portion of AAVS1 homology arm from prior cloning" /note="color: #800000; direction: LEFT" misc_feature 569..1364 /label=C13T1-RA /note="color: #c0c0c0" misc_feature 1376..2045 /label=Synthesized DNA from GeneWiz /note="color: #a6acb3" misc_signal 1376..2045 /label=SeqVer (WT AAV R) /note="color: #a6acb3; direction: RIGHT" misc_signal 1376..2045 /label=SeqVer (SV40 F) /note="color: #a6acb3; direction: RIGHT" misc_feature 1376..2045 /label=short UCOE /note="blocks chromatin remodelling/silencing p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica; -webkit-text-stroke: #000000} p.p2 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica; color: #4787ff; -webkit-text-stroke: #4787ff} span.s1 {font-kerning: none} span.s2 {text-decoration: underline ; font-kerning: none} It is part of the human HNRPA2B1-CBX3 locus. This is the short version. There is also a longer fragment, but the short one is supposed to work just as well. See also these papers: http://dx.doi.org/10.1182/blood-2006-12-060814 http://dx.doi.org/10.1038/mt.2010.132 " /note="color: #a6acb3" misc_signal 1376..2045 /label=SeqVer (WT AAV R) /note="color: #------; direction: RIGHT" misc_signal 1376..2045 /label=SeqVer (SV40 F) /note="color: #------; direction: RIGHT" promoter 2052..2429 /label=TRE3G promoter /note="3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On(R) 3G -sequence identical to Kyle Denton's AAVS1 integratable non-silencing plasmid" /note="color: #ffffff; direction: RIGHT" misc_feature 2052..2429 /label=added via PCR /note="color: #a6acb3" misc_signal 2436..3266 /label=SeqVer (WT AAV R) /note="color: #------; direction: RIGHT" misc_signal 2436..3266 /label=SeqVer (SV40 F) /note="color: #------; direction: RIGHT" misc_signal 2436..3266 /label=SeqVer (TRE 3G F) /note="color: #------; direction: RIGHT" misc_feature 2436..2444 /label=Kozac /note="color: #c0c0c0; direction: RIGHT" CDS 2445..3263 /codon_start=1 /label=hNEUROG2 /note="color: #339966" /translation="MFVKSETLELKEEEDVLVLLGSASPALAALTPLSSSADEEEEEEP GASGGARRQRGAEAGQGARGGVAAGAEGCRPARLLGLVHDCKRRPSRARAVSRGAKTAE TVQRIKKTRRLKANNRERNRMHNLNAALDALREVLPTFPEDAKLTKIETLRFAHNYIWA LTETLRLADHCGGGGGGLPGALFSEAVLLSPGGASAALSSSGDSPSPASTWSCTNSPAP SSSVSSNSTSPYSCTLSPASPAGSDMDYWQPPPPDKHRYAPHLPIARDCIL" misc_signal 3281..3834 /label=SeqVer (TRE 3G F) /note="color: #------; direction: RIGHT" misc_signal 3281..3649 /label=SeqVer (WT AAV R) /note="color: #------; direction: RIGHT" misc_signal 3281..3458 /label=SeqVer (SV40 F) /note="color: #------; direction: RIGHT" polyA_signal 3297..3427 /label=SV40polyA /label=SV40 pA /note="Common feature added/edited by Mo Mandegar Slightly longer sequence than the archieved SnapGene Version (131bp vs 122bp)" /note="color: #a6acb3; direction: RIGHT" polyA_signal complement(3771..4225) /label=RBGpolyA (minus last 72bp) /label=RBGpolyA /note="Common feature added by Mo Mandegar" /note="color: #a6acb3; direction: LEFT" CDS complement(4235..4982) /codon_start=1 /label=rtTA3G /note="Common feature added by Mo Mandegar" /note="color: #ffcc00" /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" intron 4989..5082 /label=Mini- Intron /label=Mini-Intron /note="Common feature added by Mo Mandegar" /note="color: #------" enhancer complement(5090..6337) /label=CAG /note="Common feature added by Mo Mandegar" /note="This reverse directional feature has 2 segments: 1: 5090 .. 6059 / #ffffff / Enhancer Element 2: 6060 .. 6337 / #ffffff / Chicken beta-actin Promoter" enhancer 6339..6718 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" polyA_signal complement(6724..6948) /label=bGHpolyA /note="bovine growth hormone polyadenylation signal Common feature added/modified by Mo Mandegar" /note="color: #a6acb3; direction: LEFT" misc_signal complement(6886..9192) /label=SeqVer (AAV F) /note="color: #------; direction: LEFT" misc_signal complement(6949..8414) /label=SeqVer (AAV F) /note="color: #------; direction: LEFT" misc_signal complement(6949..8414) /label=SeqVer (AAV F) /note="color: #------; direction: LEFT" protein_bind complement(6970..7003) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." /note="color: #31849b; direction: LEFT" misc_signal complement(6970..7003) /label=SeqVer (AAV F) /note="color: #------; direction: LEFT" CDS complement(7010..7073) /codon_start=1 /label=mCherry /note="color: #ff0000" /translation="TIVEQYERAEGRHSTGGMDE" CDS complement(7077..7784) /codon_start=1 /label=Optimized mApple /note="color: #ff0000" /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA EGRHSTGGMDELYK" misc_feature 7785..7790 /label=Kozak /note="color: #a6acb3" misc_feature 7801..7818 /label=Feature 62 /note="color: #a6acb3" misc_feature complement(7839..7865) /label=mycNLS /note="color: #fc6665; direction: LEFT" misc_feature complement(7881..7907) /label=mycNLS /note="color: #fc6665; direction: LEFT" misc_feature complement(7908..7973) /label=T2A /note="color: #407f02; direction: LEFT" misc_signal complement(7983..8402) /label=SeqVer (AAV F) /note="color: #------; direction: LEFT" CDS complement(7983..8402) /codon_start=1 /label=Bsd /note="Common feature added by Mo Mandegar" /note="color: #ff6600" /translation="MKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIIS AVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCR ELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN" misc_signal complement(8415..9192) /label=SeqVer (AAV F) /note="color: #------; direction: LEFT" promoter 8415..8957 /label=EF-1alpha promoter /note="color: #ffffff" misc_signal 8964..9188 /label=SeqVer (CMV 1R) /note="color: #------; direction: RIGHT" misc_signal complement(8964..9188) /label=SeqVer (Puro 1F) /note="color: #------; direction: LEFT" polyA_signal complement(8964..9188) /label=bGHpolyA /note="bovine growth hormone polyadenylation signal Common feature added/modified by Mo Mandegar" /note="color: #a6acb3; direction: LEFT" misc_feature 9193..10090 /label=PCR #A2 /note="color: #a6acb3" CDS complement(9193..9984) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /note="color: #ffcc00" /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" CDS complement(9988..10041) /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /note="color: #ffffff" /translation="EGRGSLLTCGDVEENPGP" misc_feature 10065..10090 /label=Splice acceptor /note="color: #a6acb3" protein_bind complement(10091..10124) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." /note="color: #31849b; direction: LEFT" misc_feature 10091..10092 /label=inserted via PCR primer /note="color: #a6acb3" misc_feature 10127..10964 /label=PCR #A1 product /note="color: #a6acb3" misc_feature 10133..10964 /label=C13-LA /note="color: #c0c0c0" CDS 10982..11842 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 10982 .. 11050 / #ccffcc / signal sequence 2: 11051 .. 11842 / #ccffcc" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 12013..365 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" ORIGIN 1 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 61 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc gaattcgccc ttagagcaga 421 gccaggaacc cctgtaggga aggggcagga gagccagggg catgagatgg tggacgagga 481 agggggacag ggaagcctga gcgcctctcc tgggcttgcc aaggactcaa acccagaagc 541 ccagagcagg gccttaggga agcgggacgg ctctgttgga gagtccagta ttgaattagt 601 gatagataca cagaaaaaaa gcaaattgaa taggtgaatg gataaacaaa ctgtggaaca 661 tccagacaat gaaatattca ccaataaaaa gaaatgagct atcaagccac attggctatg 721 cccttcacat ttaaggagga accttaaatg catattgcca aattttaaaa gctagtctga 781 aaatgctaca gactgtatga ttccaattat atgacatttt agaaaaggca aaactataga 841 gatcagtggt tgccagcggc tcatggaggc aggatgaatt ggtggaacac agggatgctt 901 tcagggtaat gaaaatatct tgtgtgatcc tgtaataatg gatacgtggc ataatgcatt 961 tgttaaaacc tgtaaactag ccaggtatag tggctttttt gcacctatag tctcagctac 1021 ttggtaggct gagacaggaa gatcacttga gcccaggact tcaagaccag cctgggtaat 1081 atagtaagac cccatctcta ttttttaaag aaattaagaa agaaaagaaa cccatcaact 1141 gtataacaca aagaatcgta atgtaaacta cagtcctgag taatgaaaca tttttaaaga 1201 aataaataaa ttcaaggcaa ttattaactt caggaaaaac agtaaagtaa acataagtat 1261 acaaatcaaa catggctcag ttgtgaaaat atttacaagg tcactttaag taacattgaa 1321 tgttgatata atcccaaaat atatttatgt tggaaggatg aggagattta attaagggag 1381 gtggtccctg cagttacgcc aatgataacc cccgccagaa aaatcttagt agccttccct 1441 ttttgttttc cgtgccccaa ctcggcggat tgactcggcc ccttccggaa acacccgaat 1501 caacttctag tcaaattatt gttcacgccg caatgaccca cccctggccc gcgtctgtgg 1561 aactgacccc tggtgtacag gagagttcgc tgctgaaagt ggtcccaaag gggtactagt 1621 ttttaagctc ccaactcccc ctcccccagc gtctggagga ttccacaccc tcgcaccgca 1681 ggggcgagga agtgggcgga gtccggtttt ggcgccagcc gctgaggctg ccaagcagaa 1741 aagccaccgc tgaggagact ccggtcactg tcctcgcccc gcctccccct tccctcccct 1801 tggggaccac cgggcgccac gccgcgaacg gtaagtgccg cggtcgtcgg cgcctccgcc 1861 ctccccctag ggccccaatt cccagcgggc gcggcgcgcg gcccctcccc ccgccgggcg 1921 cgcgcccgct gccccgccct tcgtggccgc ccggcgtggg cggtgccacc cctccccccg 1981 gcggccccgc gcgcagctcc cggctccctc ccccttcgga tgtggcttga gctgtaggcg 2041 cggagggtac cagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta 2101 tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga 2161 gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag 2221 aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta 2281 tcagtgatag agaacgtata agctttaggc gtgtacggtg ggcgcctata aaagcagagc 2341 tcgtttagtg aaccgtcaga tcgcctggag caattccaca acacttttgt cttataccaa 2401 ctttccgtac cacttcctac cctcgtaaag gatccgccgc caccatgttc gtcaaatccg 2461 agaccttgga gttgaaggag gaagaggacg tgttagtgct gctcggatcg gcctcccccg 2521 ccttggcggc cctgaccccg ctgtcatcca gcgccgacga agaagaggag gaggagccgg 2581 gcgcgtcagg cggggcgcgt cggcagcgcg gggctgaggc cgggcagggg gcgcggggcg 2641 gcgtggctgc gggtgcggag ggctgccggc ccgcacggct gctgggtctg gtacacgatt 2701 gcaaacggcg cccttcccgg gcgcgggccg tctcccgagg cgccaagacg gccgagacgg 2761 tgcagcgcat caagaagacc cgtagactga aggccaacaa ccgcgagcga aaccgcatgc 2821 acaacctcaa cgcggcactg gacgcgctgc gcgaggtgct ccccacgttc cccgaggacg 2881 ccaagctcac caagatcgag accctgcgct tcgcccacaa ctacatctgg gcactcaccg 2941 agaccctgcg cctggcggat cactgcgggg gcggcggcgg gggcctgccg ggggcgctct 3001 tctccgaggc agtgttgctg agcccgggag gcgccagcgc cgccctgagc agcagcggag 3061 acagcccctc gcccgcctcc acgtggagtt gcaccaacag ccccgcgccg tcctcctccg 3121 tgtcctccaa ttccacctcc ccctacagct gcactttatc gcccgccagc ccggccgggt 3181 cagacatgga ctattggcag cccccacctc ccgacaagca ccgctatgca cctcacctcc 3241 ccatagccag ggattgtatc ttgtaagttt aaactcgcga atcgatcgcc caccccaact 3301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 3361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 3421 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 3481 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 3541 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 3601 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 3661 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 3721 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggacggccgc acatgtaagg 3781 aaaattttag ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa aaaatctaac 3841 ctcaagtcaa ggcttttcta tggaataagg aatggacagc agggggctgt ttcatatact 3901 gatgacctct ttatagccaa cctttgttca tggcagccag catatgggca tatgttgcca 3961 aactctaaac caaatactca ttctgatgtt ttaaatgatt tgccctccca tatgtccttc 4021 cgagtgagag acacaaaaaa ttccaacaca ctattgcaat gaaaataaat ttcctttatt 4081 agccagaagt cagatgctca aggggcttca tgatgtcccc ataatttttg gcagagggaa 4141 aaagatctca gtggtatttg tgagccaggg cattggccac accagccacc accttctgat 4201 aggcagcctg cacctgagga gtgaattcac gcgttttagc ctggcagcat atcgagatcg 4261 aagtcatcga gagcgtcagc agggagcata tccagatcaa aatcatccag ggcatcggcg 4321 ggcagcatgt ccaggtcgaa gtcgtccagg gcgtcggtgg gtccgccgct ctcgcacttc 4381 agctgcttct cgaggccgca gatgatcagt tccaggccga acaggaaggc gggctcggcg 4441 ccctgtctgt cgaacagctc gatggcctgc ttcagcaggg ggggcatgct gtcggtggtg 4501 ggtgtctctc tttcctcttt ggcgacctgg tgttcctgtt cttccagcac gcagcccagg 4561 gtgaagtggc ccacggcgct cagggcgtac agggcgtttt ccaggctgaa gccctgctgg 4621 cacaggaagg ccagctggtt ttccagtgtc tcgtactgct tctcggtggg tctggtgccc 4681 aggtgcactt tggcgccgtc ccggtggctc agcagggcgc atctgtagct cttggcgttg 4741 ttccgcagga agtcctgcca gctctcgcct tccagagggc agctgtgggt gtggtgccgg 4801 tccagcatct cgatgggcag ggcgtccagc agggcccgct tgttcttcac gtgccagtac 4861 agggtgggct gttccacgcc cagtttctgg gccagcttcc gggtggtcag gccctcgatg 4921 cccacgccgt tcagcagttc cagggcgctg ttgatcactt tgctcttgtc cagccggctc 4981 atgtcgacat tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag 5041 ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac 5101 acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg 5161 acagccccga ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc 5221 cccgcccatt tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt 5281 gcggcggcgc ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc 5341 cctctcgcac gattaccata aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc 5401 ggcgctccgg gggccgccgc gcccctcccc cgagccctcc ccggcccgag gcggccccgc 5461 cccgcccggc acccccacct gccgccaccc cccgcccggc acggcgagcc ccgcgccacg 5521 ccccgtacgg agccccgcac ccgaagccgg gccgtgctca gcaactcggg gaggggggtg 5581 cagggggggt tgcagcccga ccgacgcgcc cacaccccct gctcaccccc ccacgcacac 5641 accccgcacg cagcctttgt tcccctcgca gccccccccg caccgcgggg caccgccccc 5701 ggccgcgctc ccctcgcgca cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg 5761 ctcacagccg ccgggcagcg cggagccgca cgcggcgctc cccacgcaca cacacacgca 5821 cgcacccccc gagccgctcc ccccgcacaa agggccctcc cggagcccct caaggctttc 5881 acgcagccac agaaaagaaa caagccgtca ttaaaccaag cgctaattac agcccggagg 5941 agaagggccg tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg 6001 gcggcgcgag gcggcggcgg agcggggcac ggggcgaagg cagcgtcgca gcgactcccc 6061 gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga 6121 aactttcgga gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc 6181 cgcccctcgc cccgccccgc cccgcctggc gcgcgccccc cccccccccc cgcccccatc 6241 gctgcacaaa ataattaaaa aataaataaa tacaaaattg ggggtgggga ggggggggag 6301 atggggagag tgaagcagaa cgtggggctc acctcgacca tggtaatagc gatgactaat 6361 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag 6421 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga 6481 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc 6541 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg 6601 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg 6661 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcga 6721 gacccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct 6781 tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg 6841 caatttcctc attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag 6901 gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc 6961 agcgggttta taacttcgta taatgtatgc tatacgaagt tatggtacct tactcgtcca 7021 tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg gtgttacttg 7081 tacagctcgt ccattccgcc tgtgctgtgt ctgccttcgg ctctctcgta ctgttccacg 7141 atggtgtagt cctcgttgtg ggacacgatg tccagcttga tatccacgat gtaggcgcca 7201 ggcagctgca cgggtttctt ggccttgtag gtggttttca cttcggcggc atagtggccg 7261 ccatccttca gcttcagccg cttcttgatc tcggacttca gggcgccatc ctcagggtac 7321 attctttcct cgctggcttc ccagcccatg gtctttttct gcatcacggg gccatcagag 7381 ggaaagttgg tgccccgcag cttcactttg tagatgaaca cgccgtcctg cagagagcta 7441 tcctggttca cgtggatgat gccgccgtcc tcgaagttca tcactctctc ccatctgaag 7501 ccctcgggga agctcagctt gaagtagtcg ggaatgtcgg cggggtgctt gatgtacacc 7561 ttgctgccgt acataaactg agggctcaga atgtcccagg caaaaggcag agggccgcct 7621 ttggtcactt tcagcttggc ggtctgaaaa gcctcgtagg gtctgccctc gccttcgcct 7681 tcaatctcga actcgtggcc gttcacgctg ccttccatgt gcaccttgaa ccgcatgaat 7741 tctttgatga tggccatgtt gttctcttcg cccttggaca ccatggtggc gaccggtagc 7801 gtggcgaccg gggtggcggt ggctccccct gaccctccat ccaacttaac gcgctttgca 7861 gcaggacctc cagaaccgcc gtcgagtttg acccgtttag cggcagggac cggtgggcca 7921 ggattctcct cgacgtcacc gcatgttagc agacttcctc tgccctctcc cgatcccgat 7981 ccatttcggg tatatttgag tggaatgagt tcttcaatcg tagttttgac taacttgcca 8041 ttcatttcta ttaacacaaa acaatctggt gcatagtctg aaatcaactc cctacacata 8101 ccacaaggac ttaccactcg aatacttcta tctacttcgt cagaataagg gtgtctaaca 8161 gctacaatcg tgtcaaaatc cttttgtcca ttcgaaactg cactaccaat cgcaatggct 8221 tctgcacaaa cagttactcg tcctatatac gcttcaatat gtactgccga aatgatttct 8281 cctgttttcg tacgaattgc cgctcccaca tgatgtttat tatcctcata aagcattgta 8341 atcttctctg tcgctacttc tactaattct agatcctgct gggagatgtt gaaggtcttc 8401 atggtggcgc tagcgtaggc gccggtcaca gcttggatct gtaacggcgc agaacagaaa 8461 acgaaacaaa gacgtagagt tgagcaagca gggtcaggca aagcgtggag agccggctga 8521 gtctaggtag gctccaaggg agcgccggac aaaggcccgg tctcgacctg agctttaaac 8581 ttacctagac ggcggacgca gttcaggagg caccacaggc gggaggcggc agaacgcgac 8641 tcaaccggcg tggatggcgg cctcaggtag ggcggcgggc gcgtgaagga gagatgcgag 8701 cccctcgaag cttcagctgt gttctggcgg caaacccgtt gcgaaaaaga acgttcacgg 8761 cgactactgc acttatatac ggttctcccc caccctcggg aaaaaggcgg agccagtaca 8821 cgacatcact ttcccagttt accccgcgcc accttctcta ggcaccggtt caattgccga 8881 cccctccccc caacttctcg gggactgtgg gcgatgtgcg ctctgcccac tgacgggcac 8941 cggagcgatc gcagatcatg catccataga gcccaccgca tccccagcat gcctgctatt 9001 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca 9061 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc 9121 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga 9181 aggcacaggt ttttagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc 9241 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc 9301 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc 9361 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc 9421 gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag 9481 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc 9541 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt 9601 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc 9661 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc 9721 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag 9781 ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt 9841 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc 9901 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc 9961 tgcgtgcaat ccatcttgtt caatcctagg gccgggattc tcctccacgt caccgcatgt 10021 tagaagactt cctctgccct ctccgctgcc agatctctcg aggccctgtg ggaggaagag 10081 aagaggtcag ataacttcgt ataatgtatg ctatacgaag ttatgcggcc gctgggagaa 10141 gggtaagaga gctcaatcct catcttacat agaaggaagt cagtagataa tatcttacat 10201 ttaaaagtca agaaataaca gtctgaccat actatctaga aatatggaag ttaacagtaa 10261 ggaagttaaa acagttgaaa gtagtttcgt ctagaaagaa agacttgggg aagaacaaag 10321 aaggctaatt ttcattatga gctttattgt aacagaaaag agaccactaa ctcagctagg 10381 ttttcccaat tcctcaacac tttggggcac agtgtttagt cagagctgtg atcactctct 10441 gagcacatgg attgtatctt aattttcatc tcattgaaaa tatgtaccat ttgtccaagt 10501 ggaaactggg ctgaagcatg actgtgacca ccaccaaaga aatggctaga ctcgctaagg 10561 tctgctgggg tgacccgtga ttgtgttgtg cttgatcttg ttgagcatgg ctcttttaag 10621 tcaccatacc tgcccctgcc aatgcaccaa acaccaactt aaagggactt tcagcttttg 10681 taagtcagtc tcagccttac ttctgaacca tttctttgac ttctttcatg actctgatcc 10741 tgtcccagtt ttaagcctcc cagccaccag tcatgctgtt ttgctctgct atttacattc 10801 ttctctacct actttggact tgatggtatg cctactatat tagttattta ttgctgtgta 10861 acaaaccact ctgaaacata gtggcttaca accacaacca ttttatttta ttatgaaact 10921 gtgggtcaga atgtatattg gcccattggc atgattctcc tgctattgaa aaaggaagag 10981 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 11041 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 11101 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 11161 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 11221 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 11281 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 11341 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 11401 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 11461 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 11521 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 11581 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 11641 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 11701 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 11761 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 11821 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 11881 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 11941 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 12001 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 12061 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 12121 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 12181 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcc //