LOCUS       14xUAS-CheRiff-tdT_Tol1.xdna    6944 bp      DNA     circular   25/02/2019
DEFINITION  
TITLE       LOCUS pTol1-UAS:CheRiff-tdTomato
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
            COMMENT     SerialCloner_Type=DNA
            COMMENT     SerialCloner_Comments=[DEFINITION]:  - [LOCUS]: Construct_1
            COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             1564..2511
                     /label=CheRiff
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             2527..3957
                     /label=tdTomato
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(4548..4565)
                     /label=M13-fwd
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      6152..6780
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     204..224
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4636..4704
                     /label=LacZ alpha
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    176..198
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             5341..6000
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        5073..5101
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        142..171
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1558..1563
                     /label=Kozak sequence
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    869..1557
                     /label=UBC intron
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    425..785
                     /label=14xUAS repeats
                     /SerialCloner_Color=&h80FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    785..825
                     /label=E1b basal promoter
                     /SerialCloner_Color=&hFF8080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    826..849
                     /label=Carp b-actin xcrn
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    255..413
                     /label=tol1 left
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3980..4400
                     /label=afp 3' UTR
                     /SerialCloner_Color=&h00CA00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4426..4561
                     /label=tol1 right
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN

        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atggtccctt tagccagtag cggttctagg cacgggccgt ccgggcggtg gcctggggcg
      301 gaaaactgaa ggggggcggc accggcggct cagcccttgt aatatattat atgcaccact
      361 attggtttac ttatgtcaca gtttgtaagt ttgtaacagc ctgaacctgg atcagcttag
      421 gcctccaagg cggagtactg tcctccgggc tggcggagta ctgtcctccg gcaaggtcgg
      481 agtactgtcc tccgacacta gaggtcggag tactgtcctc cgacgcaagg cggagtactg
      541 tcctccgggc tgcggagtac tgtcctccgg caaggtcgga gtactgtcct ccgacactag
      601 aggtcggagt actgtcctcc gacgcaaggt cggagtactg tcctccgaca ctagaggtcg
      661 gagtactgtc ctccgacgca aggtcggagt actgtcctcc gacactagag gtcggagtac
      721 tgtcctccga cgcaaggcgg agtactgtcc tccgggctgg cggagtactg tcctccggca
      781 agggtcgact ctagagggta tataatggat cccatcgcgt ctcagcctca ctttgagctc
      841 ctccacacga attcgatact aaggatcctt gtttgtgagt atgatgttaa atataagcaa
      901 tcgacgtttg ttttgtcgtt tgttaaatct agcaatgttt tatttcaatt aaagtgttaa
      961 ccggttcatt taaaggtgta tttctgaatg agctaacgta ttagctttta acgctagccg
     1021 gactgtttgg ttttgaggat caataaacca acgttttttt ctgatttatt ttgctttaaa
     1081 atataaaggt ataattcgtc gtcgagaatt ttgggttttg ttcgagttta gaaatttggc
     1141 ggcgtttttt tgtaccgcga aacagggcgg tggttcggtg agcttctcaa ttcatctgct
     1201 tgacaaacgg ttttcggcaa ctgtaaatgc gctatgatgc ttttattctt ttcagcgtgt
     1261 aactttgtta aatataattg actttccgac tatcgacgtt cgtttcatct ccgtgttatt
     1321 gtttaatggc cattttctgt ggtaaactgt cgccattttg ttttcagtcg tccgtttgtt
     1381 ccgcccttac tcacagctgt gtcagcatta gtgatgactg ttctcacctg gcctgtttgc
     1441 tttcattatt gatgaagagc tttctagaga attctgaagc tcgtctttgt cctgaattca
     1501 tctgtattca tttaaaaaaa aaaattaacc ttaatttttt tttcttctca gcgtaaagcc
     1561 accATGGGCG GAGCTCCTGC TCCAGACGCT CACAGCGCCC CACCTGGAAA CGATTCTGCC
     1621 GGAGGCAGTG AGTACCATGC CCCAGCTGGA TATCAAGTGA ATCCACCCTA CCACCCCGTG
     1681 CATGGGTATG AGGAACAGTG CAGCTCCATC TACATCTACT ATGGGGCCCT GTGGGAGCAG
     1741 GAAACAGCTA GGGGCTTCCA GTGGTTTGCC GTGTTCCTGT CTGCCCTGTT TCTGGCTTTC
     1801 TACGGCTGGC ACGCCTATAA GGCCAGCGTG GGATGGGAGG AAGTGTACGT GTGCTCCGTG
     1861 GAGCTGATCA AAGTGATTCT GGAGATCTAT TTCGAGTTCA CCAGTCCTGC TATGCTGTTC
     1921 CTGTACGGAG GGAACATTAC CCCATGGCTG AGATATGCCG AATGGCTGCT GACATGTCCC
     1981 GTGATCCTGA TTCATCTGTC TAACATCACC GGCCTGAGTG AGGCATACAA TAAGCGGACA
     2041 ATGGCTCTGC TGGTGTCCGA CCTGGGAACT ATTTGCATGG GAGTGACAGC CGCTCTGGCC
     2101 ACTGGGTGGG TGAAGTGGCT GTTTTACTGT ATCGGCCTGG TGTATGGAAC CCAGACATTC
     2161 TACAACGCTG GAATCATCTA CGTGGAGTCT TACTATATCA TGCCTGCCGG CGGCTGTAAG
     2221 AAACTGGTGC TGGCCATGAC TGCCGTGTAC TATTCTAGTT GGCTGATGTT TCCCGGCCTG
     2281 TTCATCTTTG GGCCTGAAGG CATGCACACC CTGAGCGTGG CTGGGTCCAC TATTGGCCAT
     2341 ACCATCGCCG ACCTGCTGTC CAAGAATATT TGGGGACTGC TGGGGCACTT CCTGCGGATC
     2401 AAAATTCACG AGCATATCAT TATGTACGGC GATATCAGGA GACCAGTGAG CTCCCAGTTT
     2461 CTGGGACGCA AGGTGGACGT GCTGGCCTTC GTGACAGAGG AAGATAAAGT GGCGGCACCG
     2521 GTAGTAGCAG TGAGTAAGGG CGAGGAAGTG ATCAAAGAGT TCATGCGGTT TAAGGTGAGA
     2581 ATGGAAGGAA GCATGAACGG CCACGAGTTC GAAATTGAGG GAGAAGGAGA GGGACGGCCC
     2641 TACGAGGGCA CCCAGACAGC CAAGCTGAAA GTGACAAAGG GCGGGCCTCT GCCATTCGCT
     2701 TGGGACATCC TGAGCCCACA GTTTATGTAC GGCTCCAAGG CCTATGTGAA ACATCCAGCT
     2761 GACATTCCCG ATTATAAGAA ACTGAGCTTC CCCGAGGGGT TTAAGTGGGA AAGAGTGATG
     2821 AACTTCGAGG ACGGAGGCCT GGTGACTGTG ACCCAGGACA GCTCCCTGCA GGATGGGACC
     2881 CTGATCTACA AGGTGAAAAT GAGAGGGACA AATTTTCCCC CTGATGGACC TGTGATGCAG
     2941 AAGAAAACTA TGGGATGGGA GGCCTCCACC GAAAGGCTGT ATCCACGCGA CGGGGTGCTG
     3001 AAAGGAGAAA TCCACCAGGC TCTGAAGCTG AAAGATGGGG GACATTACCT GGTGGAGTTC
     3061 AAGACAATCT ACATGGCCAA GAAACCTGTG CAGCTGCCAG GCTACTATTA CGTGGACACA
     3121 AAACTGGATA TCACTTCACA CAACGAGGAC TACACTATTG TGGAGCAGTA TGAACGGAGC
     3181 GAGGGGAGAC ACCATCTGTT CCTGGGCCAT GGGACTGGAA GTACCGGCTC AGGGTCTAGT
     3241 GGAACCGCCT CAAGCGAGGA TAACAATATG GCTGTGATCA AAGAGTTCAT GAGGTTTAAG
     3301 GTGCGCATGG AGGGCAGCAT GAATGGGCAC GAATTTGAGA TTGAAGGAGA GGGCGAAGGG
     3361 AGGCCTTACG AGGGCACACA GACTGCCAAG CTGAAAGTGA CCAAGGGAGG ACCACTGCCT
     3421 TTCGCTTGGG ATATCCTGTC TCCTCAGTTT ATGTACGGAA GTAAGGCCTA TGTCAAGCAT
     3481 CCCGCTGACA TTCCTGATTA CAAGAAACTG TCTTTCCCAG AGGGCTTTAA GTGGGAGAGA
     3541 GTGATGAATT TTGAAGATGG AGGCCTGGTG ACCGTGACAC AGGACTCCTC TCTGCAGGAT
     3601 GGCACTCTGA TCTACAAAGT CAAAATGCGC GGCACCAATT TTCCACCCGA TGGGCCCGTG
     3661 ATGCAGAAGA AAACAATGGG GTGGGAGGCC AGCACTGAAC GGCTGTATCC TAGAGACGGA
     3721 GTGCTGAAGG GCGAAATCCA CCAGGCCCTG AAGCTGAAAG ACGGCGGCCA CTACCTGGTG
     3781 GAGTTCAAAA CCATCTACAT GGCCAAGAAA CCAGTGCAGC TGCCCGGCTA TTACTATGTG
     3841 GACACCAAGC TGGATATCAC ATCCCACAAT GAAGACTACA CCATTGTGGA ACAGTATGAG
     3901 AGGTCTGAAG GACGCCACCA TCTGTTTCTG TACGGCATGG ATGAGCTGTA TAAGTAAaca
     3961 tggagatctc gagactagta attaagtctc agccaccgtt aactgaacat gtcaaaacct
     4021 gtggagactg ttgagatttg atgttctgaa aagataaagc ctataaataa aatgttgccc
     4081 aaatttcctg cctgatgttt ttctttgtct ttgctacatg gctttgctgc tcggatcggc
     4141 tcactctgtg tatgccacgt tcactttgta ctctccttct cacggtaggt ttattatttt
     4201 tagatgtgca gttagtttct gtgaaataac acaccacaca ctgatattgt ctgtgcattg
     4261 acttggtgag tgcacattgt ttttgatctt gacatattta tatttgattg atcaggtgaa
     4321 ctgtgtgaat ctaaagtgct ccatacagat gttctgcatt gaaaatattc tcattttatt
     4381 agtggaagtg agtgtatgcc agcggccgcg gtaccctgca gtctagacat ttcagttgac
     4441 gaagacaaac aaagttctgt tgtgactatg gggggggggg gcgcctgggg atggtctcgc
     4501 ccggggagta attcagggta gaaccgccac tgcctttagc gacattcact ggccgtcgtt
     4561 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4621 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4681 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4741 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     4801 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     4861 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     4921 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     4981 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     5041 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5101 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5161 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5221 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5281 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5341 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5401 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5461 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5521 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5581 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5641 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5701 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5761 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5821 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5881 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5941 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6001 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6061 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6121 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6181 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6241 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6301 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6361 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6421 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6481 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6541 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6601 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6661 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6721 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6781 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6841 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6901 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aaga
//
