LOCUS 14xUAS-GtACR2-tdT_Tol1.xdna 6869 bp DNA circular 25/02/2019 DEFINITION TITLE LOCUS pTol1-UAS:GtACR2-tdTomato SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments=[DEFINITION]: - [LOCUS]: Construct_1 COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers CDS 1564..2436 /label=GtACR2 /SerialCloner_Color=&h0080FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True CDS 2452..3882 /label=tdTomato /SerialCloner_Color=&hFF0000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True primer_bind complement(4473..4490) /label=M13-fwd /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin 6077..6705 /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind 204..224 /label=M13-rev /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 4561..4629 /label=LacZ alpha /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_binding 176..198 /label=LacO /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 5266..5925 /label=AmpR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter 4998..5026 /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter 142..171 /label=lac /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 1558..1563 /label=Kozak sequence /SerialCloner_Color=&hFFFF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 869..1557 /label=UBC intron /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 425..785 /label=14xUAS repeats /SerialCloner_Color=&h80FFFF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 785..825 /label=E1b basal promoter /SerialCloner_Color=&hFF8080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 826..849 /label=Carp b-actin xcrn /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 255..413 /label=tol1 left /SerialCloner_Color=&hFF00FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 3905..4325 /label=afp 3' UTR /SerialCloner_Color=&h00CA00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 4351..4486 /label=tol1 right /SerialCloner_Color=&hFF00FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc 241 atggtccctt tagccagtag cggttctagg cacgggccgt ccgggcggtg gcctggggcg 301 gaaaactgaa ggggggcggc accggcggct cagcccttgt aatatattat atgcaccact 361 attggtttac ttatgtcaca gtttgtaagt ttgtaacagc ctgaacctgg atcagcttag 421 gcctccaagg cggagtactg tcctccgggc tggcggagta ctgtcctccg gcaaggtcgg 481 agtactgtcc tccgacacta gaggtcggag tactgtcctc cgacgcaagg cggagtactg 541 tcctccgggc tgcggagtac tgtcctccgg caaggtcgga gtactgtcct ccgacactag 601 aggtcggagt actgtcctcc gacgcaaggt cggagtactg tcctccgaca ctagaggtcg 661 gagtactgtc ctccgacgca aggtcggagt actgtcctcc gacactagag gtcggagtac 721 tgtcctccga cgcaaggcgg agtactgtcc tccgggctgg cggagtactg tcctccggca 781 agggtcgact ctagagggta tataatggat cccatcgcgt ctcagcctca ctttgagctc 841 ctccacacga attcgatact aaggatcctt gtttgtgagt atgatgttaa atataagcaa 901 tcgacgtttg ttttgtcgtt tgttaaatct agcaatgttt tatttcaatt aaagtgttaa 961 ccggttcatt taaaggtgta tttctgaatg agctaacgta ttagctttta acgctagccg 1021 gactgtttgg ttttgaggat caataaacca acgttttttt ctgatttatt ttgctttaaa 1081 atataaaggt ataattcgtc gtcgagaatt ttgggttttg ttcgagttta gaaatttggc 1141 ggcgtttttt tgtaccgcga aacagggcgg tggttcggtg agcttctcaa ttcatctgct 1201 tgacaaacgg ttttcggcaa ctgtaaatgc gctatgatgc ttttattctt ttcagcgtgt 1261 aactttgtta aatataattg actttccgac tatcgacgtt cgtttcatct ccgtgttatt 1321 gtttaatggc cattttctgt ggtaaactgt cgccattttg ttttcagtcg tccgtttgtt 1381 ccgcccttac tcacagctgt gtcagcatta gtgatgactg ttctcacctg gcctgtttgc 1441 tttcattatt gatgaagagc tttctagaga attctgaagc tcgtctttgt cctgaattca 1501 tctgtattca tttaaaaaaa aaaattaacc ttaatttttt tttcttctca gcgtaaagcc 1561 accATGGCCT CCCAGGTCGT GTACGGCGAG TGGGCCAGCA CCCACACAGA GTGCTACAAC 1621 ATGAGCAGGA TCGACAGCAC CTTCGTGAGC CTGCTGCAGC TGGTGTGGGC CGTGGTGAGC 1681 GGATGCCAGA CCATCTTCAT GATCAGCAGG GCCCCCAAGG TGCCCTGGGA GAGCGTGTAC 1741 CTGCCCTTCG TGGAGTCCAT CACATACGCC CTGGCCAGCA CCGGCAATGG CACACTGCAG 1801 ATGAGGGACG GCAGGTTCTT TCCCTGGTCC AGGATGGCCA GCTGGCTGTG CACATGCCCC 1861 ATTATGCTGG GCCAGATCTC CAACATGGCT CTGGTGAAGT ACAAGAGCAT CCCTCTGAAC 1921 CCCATCGCCC AGGCCGCCAG CATTATCAGG GTGGTGATGG GCATTACCGC TACCATCTCC 1981 CCCGCCGAGT ACATGAAGTG GCTGTTCTTC TTCTTCGGCG CTACCTGCCT GGTCTTCGAA 2041 TACAGCGTGG TGTTCACCAT CTTCCAGGTG GGACTGTACG GCTTCGAGTC CGTGGGCACC 2101 CCTCTGGCCC AGAAGGTGGT GGTGAGGATC AAGATGCTGA GGCTGATCTT CTTCATCGCC 2161 TGGACCATGT TCCCCATCGT GTGGCTGATT TCCCCCACCG GCGTGTGCGT GATCCACGAG 2221 AACGTGAGCG CCATCCTGTA TCTGCTCGCC GACGGCCTGT GCAAGAACAC CTACGGCGTG 2281 ATCCTGTGGT CCACCGCCTG GGGAGTGCTC GAGGGAAAGT GGGACCCGGC TTGCCTCCCT 2341 GGCCAGGAGA AGCCTGAGGC CGACGACCCC TTCGGACTGA ACCACGAGAA GAATGCCCCC 2401 CCCAACGACG AGGTCAACAT CAGAATGTTC GGCAGGGCGG CACCGGTAGT AGCAGTGAGT 2461 AAGGGCGAGG AAGTGATCAA AGAGTTCATG CGGTTTAAGG TGAGAATGGA AGGAAGCATG 2521 AACGGCCACG AGTTCGAAAT TGAGGGAGAA GGAGAGGGAC GGCCCTACGA GGGCACCCAG 2581 ACAGCCAAGC TGAAAGTGAC AAAGGGCGGG CCTCTGCCAT TCGCTTGGGA CATCCTGAGC 2641 CCACAGTTTA TGTACGGCTC CAAGGCCTAT GTGAAACATC CAGCTGACAT TCCCGATTAT 2701 AAGAAACTGA GCTTCCCCGA GGGGTTTAAG TGGGAAAGAG TGATGAACTT CGAGGACGGA 2761 GGCCTGGTGA CTGTGACCCA GGACAGCTCC CTGCAGGATG GGACCCTGAT CTACAAGGTG 2821 AAAATGAGAG GGACAAATTT TCCCCCTGAT GGACCTGTGA TGCAGAAGAA AACTATGGGA 2881 TGGGAGGCCT CCACCGAAAG GCTGTATCCA CGCGACGGGG TGCTGAAAGG AGAAATCCAC 2941 CAGGCTCTGA AGCTGAAAGA TGGGGGACAT TACCTGGTGG AGTTCAAGAC AATCTACATG 3001 GCCAAGAAAC CTGTGCAGCT GCCAGGCTAC TATTACGTGG ACACAAAACT GGATATCACT 3061 TCACACAACG AGGACTACAC TATTGTGGAG CAGTATGAAC GGAGCGAGGG GAGACACCAT 3121 CTGTTCCTGG GCCATGGGAC TGGAAGTACC GGCTCAGGGT CTAGTGGAAC CGCCTCAAGC 3181 GAGGATAACA ATATGGCTGT GATCAAAGAG TTCATGAGGT TTAAGGTGCG CATGGAGGGC 3241 AGCATGAATG GGCACGAATT TGAGATTGAA GGAGAGGGCG AAGGGAGGCC TTACGAGGGC 3301 ACACAGACTG CCAAGCTGAA AGTGACCAAG GGAGGACCAC TGCCTTTCGC TTGGGATATC 3361 CTGTCTCCTC AGTTTATGTA CGGAAGTAAG GCCTATGTCA AGCATCCCGC TGACATTCCT 3421 GATTACAAGA AACTGTCTTT CCCAGAGGGC TTTAAGTGGG AGAGAGTGAT GAATTTTGAA 3481 GATGGAGGCC TGGTGACCGT GACACAGGAC TCCTCTCTGC AGGATGGCAC TCTGATCTAC 3541 AAAGTCAAAA TGCGCGGCAC CAATTTTCCA CCCGATGGGC CCGTGATGCA GAAGAAAACA 3601 ATGGGGTGGG AGGCCAGCAC TGAACGGCTG TATCCTAGAG ACGGAGTGCT GAAGGGCGAA 3661 ATCCACCAGG CCCTGAAGCT GAAAGACGGC GGCCACTACC TGGTGGAGTT CAAAACCATC 3721 TACATGGCCA AGAAACCAGT GCAGCTGCCC GGCTATTACT ATGTGGACAC CAAGCTGGAT 3781 ATCACATCCC ACAATGAAGA CTACACCATT GTGGAACAGT ATGAGAGGTC TGAAGGACGC 3841 CACCATCTGT TTCTGTACGG CATGGATGAG CTGTATAAGT AAacatggag atctcgagac 3901 tagtaattaa gtctcagcca ccgttaactg aacatgtcaa aacctgtgga gactgttgag 3961 atttgatgtt ctgaaaagat aaagcctata aataaaatgt tgcccaaatt tcctgcctga 4021 tgtttttctt tgtctttgct acatggcttt gctgctcgga tcggctcact ctgtgtatgc 4081 cacgttcact ttgtactctc cttctcacgg taggtttatt atttttagat gtgcagttag 4141 tttctgtgaa ataacacacc acacactgat attgtctgtg cattgacttg gtgagtgcac 4201 attgtttttg atcttgacat atttatattt gattgatcag gtgaactgtg tgaatctaaa 4261 gtgctccata cagatgttct gcattgaaaa tattctcatt ttattagtgg aagtgagtgt 4321 atgccagcgg ccgcggtacc ctgcagtcta gacatttcag ttgacgaaga caaacaaagt 4381 tctgttgtga ctatgggggg ggggggcgcc tggggatggt ctcgcccggg gagtaattca 4441 gggtagaacc gccactgcct ttagcgacat tcactggccg tcgttttaca acgtcgtgac 4501 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 4561 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 4621 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc 4681 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac 4741 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 4801 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 4861 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 4921 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 4981 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 5041 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 5101 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 5161 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 5221 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 5281 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 5341 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 5401 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 5461 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 5521 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 5581 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 5641 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 5701 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 5761 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 5821 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 5881 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 5941 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 6001 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 6061 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 6121 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 6181 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 6241 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 6301 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 6361 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 6421 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 6481 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 6541 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 6601 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 6661 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 6721 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 6781 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 6841 agcgagtcag tgagcgagga agcggaaga //