LOCUS       Exported                6149 bp ds-DNA     circular SYN 25-FEB-2019
DEFINITION  -.
ACCESSION   -
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 25, 2019 from SnapGene Viewer 4.1.0
            http://www.snapgene.com
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            ""Intron (1 total)"")                   #156=(CFolderPar (CGroupPar 
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                              #170=(CFolderPar (CGroupPar (CParagraph 3411 (3 
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                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
            ""Original Location Description:"" 1) #173=(CLinePar                
                                    (CParagraph 0 (0 0) 1 2 3 0 180)            
                                        "" 1812..2693"" 1))) ""ColE1 Ori""))) 
            ""Replication Origin (1 total)"") #174=(CFolderPar (CGroupPar 
            (CParagraph 44 (7 44 0) 1 1 1 0 178) (CObjectList                   
                   #165=(CFolderPar (CGroupPar (CParagraph 803 (3 #87# 0) 1 2 2 
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                            (CParagraph 0 (0 0) 1 2 3 0 180) ""Original Location
            Description:"" 1) #168=(CLinePar                                    
                (CParagraph 0 (0 0) 1 2 3 0 180)                                
                    "" 803..1026"" 1)))                                 
            ""hEF1alpha promoter"")))                         ""Promoter 
            Eukaryotic (1 total)"")                   #169=(CFolderPar 
            (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) (CGroupPar 
            (CParagraph 1221 (3 #93# 0) 1 2 2 0 327) (CObjectList #141=(CLinePar
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            ""wt AKT"")                           #142=(CFolderPar (CGroupPar 
            (CParagraph 4325 (3 #92# 0) 1 2 2 0 327) (CObjectList #143=(CLinePar
            (CParagraph 0 (0 0) 1 2 3 0 180) ""Start: 4325  End: 5134 
            (Complementary)"" 1) #144=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180)
            ""  NcoI site #1"" 1)))                                 ""Left 
            Terminus"") #136=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 
            178) (CObjectList                                   #137=(CLinePar 
            (CParagraph 0 (0 0) 1 2 3 0 180) ""  BglII site #1"" 1))) ""Right 
            Terminus"")))                         ""#2: FRAGMENT of <a 
            href=\""vnti:dnarna/Litmus%20wtAKT?sel=2787-37\"">Litmus wtAKT "")))
                            ""Component Fragments"") #138=(CFolderPar (CGroupPar
            (CParagraph 12 (6 0) 1 1 0 0 178) (CObjectList #139=(CFolderPar     
                               (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
            (CObjectList #140=(CFolderPar #150=(CLinePar (CParagraph 0 (0 0) 1 2
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            0 178) (CObjectList                           #152=(CFolderPar 
            (CGroupPar (CParagraph 1058 (3 #86# 0) 1 2 2 0 194) (CObjectList 
            #153=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) ""Start: 1058  End: 
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            (CParagraph 0 (0 0) 1 2 3 0 180) ""Original Location Description:"" 
            1) ""Original Location Description:"" 1) #145=(CLinePar (CParagraph 
            0 (0 0) 1 2 3 0 180) "" complement(2726..3535)"" 1))) ""Kan(R)""))) 
            ""CDS (2 total)"") #146=(CFolderPar (CGroupPar (CParagraph 9 (7 9 0)
            1 1 1 0 178) (CObjectList                           #147=(CFolderPar
            (CGroupPar (CParagraph 380 (3 #88# 0) 1 2 2 0 194) (CObjectList 
            #148=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) ""Start: 380   End: 
            802 "" 1) #149=(CLinePar                                         
            (CParagraph 0 (0 0) 1 2 3 0 180) ""Original Location Description:"" 
            1) 5 ""ACCGGT"" ""TGGCCA"")                   #198=(CRSFolderPar 
            (CFolderPar                          (CGroupPar (CParagraph 38811016
            (8 0) 1 1 1 0 178) (CObjectList)) ""AluI: 21 sites"") 2 2 ""AGCT"" 
            ""TCGA"") #199=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 
            38952368 (8 0) 1 1 1 0 178) (CObjectList                            
            #200=(CGroupPar (CParagraph 0 (10 #8# 0) 1 2 2 0 180) (CObjectList 
            #201=(CLinePar (CParagraph 0 (1 #8# 1) 1 2 2 0 191) "" N1: 2046 "" 
            1) #202=(CLinePar (CParagraph 0 (1 #8# 2) 1 2 2 0 191) (CParagraph 0
            (1 #4# 1) 1 2 2 0 191) "" N1: 47 "" 1))))) ""AflII: 1 site"") 1 5 
            ""CTTAAG"" ""GAATTC"")                   #194=(CRSFolderPar 
            (CFolderPar                          (CGroupPar (CParagraph 38811120
            (8 0) 1 1 1 0 178) (CObjectList)) ""AflIII: 3 sites"") 1 5 
            ""ACRYGT"" ""TGYRCA"")                   #195=(CRSFolderPar 
            (CFolderPar (CGroupPar (CParagraph 38811048 (8 0) 1 1 1 0 178) 
            (CObjectList #196=(CGroupPar (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
            (CObjectList #197=(CLinePar (CParagraph 0 (1 #6# 1) 1 2 2 0 191) "" 
            N1: 352 "" 1))))) ""AgeI: 1 site"") 1 #207=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 39003144 (8 0) 1 1 1 0 178) (CObjectList)) 
            ""AscI: 0 sites"") 2 6 ""GGCGCGCC"" ""CCGCGCGG"") #208=(CRSFolderPar
            (CFolderPar                          (CGroupPar (CParagraph 39002008
            (8 0) 1 1 1 0 178) (CObjectList #209=(CGroupPar (CParagraph 0 (10 
            #12# 0) 1 2 2 0 180) (CObjectList #210=(CLinePar (CParagraph 0 (1 
            #12# 1) 1 2 2 0 191) "" N1: 4508 "" 1))))) ""AseI: 1 site"")        
                            2 4 ""ATTAAT"" ""TAATTA"")                   
            #211=(CRSFolderPar "" N2: 2851 "" 1))))) ""ApaI: 2 sites"")         
                           5 1 ""GGGCCC"" ""CCCGGG"")                   
            #203=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 38952336 (8 0)
            1 1 1 0 178) (CObjectList #204=(CGroupPar (CParagraph 0 (10 #9# 0) 1
            2 2 0 180) (CObjectList #205=(CLinePar (CParagraph 0 (1 #9# 1) 1 2 2
            0 191) "" N1: 3848 "" 1))))) ""ApaLI: 1 site"")                     
               1 5 ""GTGCAC"" ""CACGTG"")                   #206=(CRSFolderPar 
            (CFolderPar (CGroupPar (CParagraph 39040792 (8 0) 1 1 1 0 178) 
            (CObjectList)) ""ApoI: 7 sites"") 1 5 ""RAATTY"" ""YTTAAR"") "" 
            complement(20..246)"" 1))) ""IR/DR(L)"") #180=(CFolderPar (CGroupPar
            (CParagraph 3158 (3 #90# 0) 1 2 2 0 194) (CObjectList #181=(CLinePar
            (CParagraph 0 (0 0) 1 2 3 0 180) ""Start: 3158  End: 3385"" 1) 
            #182=(CLinePar                                         (CParagraph 0
            (0 0) 1 2 3 0 180) ""IR/DR(R) has PCR induced mutations at bps 13 (T
            to A in the Ri), deleting bp 130 (\306T), 143 (C to T) and 150 (C to
            A) [these bp designations correspond to the original defined 
            IR/DR(R) from Ri to Ro, not the plasmid]"" 1) #183=(CLinePar 
            (CParagraph 0 (0 0) 1 2 3 0 180) ""Original Location Description:"" 
            1) #184=(CLinePar                          (CObjectList 
            #175=(CFolderPar                                 (CGroupPar 
            (CParagraph 20 (3 #89# 0) 1 2 2 0 194) (CObjectList #176=(CLinePar 
            (CParagraph 0 (0 0) 1 2 3 0 180) ""Start: 20    End: 246  
            (Complementary)"" 1) #177=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180)
            ""IR/DR(L) appears to have a spontaneous mutation from A to T at bp 
            44 (happens to revert back to consensus) [the bp designation 
            corresponds to the original defined IR/DR(L) from Lo to Li, not the 
            plasmid]"" 1) #178=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
            ""Original Location Description:"" 1) #179=(CLinePar (CParagraph 0 
            (0 0) 1 2 3 0 180) #189=(CRSFolderPar (CFolderPar                   
                  (CGroupPar (CParagraph 39002688 (8 0) 1 1 1 0 178) 
            (CObjectList)) ""AatII: 0 sites"") 5 1 ""GACGTC"" ""CTGCAG"")       
                       #190=(CRSFolderPar (CFolderPar                          
            (CGroupPar (CParagraph 38811224 (8 0) 1 1 1 0 178) (CObjectList)) 
            ""AciI: 33 sites"") 1 3 ""CCGC"" ""GGCG"") #191=(CRSFolderPar 
            (CFolderPar (CGroupPar (CParagraph 38811152 (8 0) 1 1 1 0 178) 
            (CObjectList                            #192=(CGroupPar (CParagraph 
            0 (10 #4# 0) 1 2 2 0 180) (CObjectList #193=(CLinePar (CParagraph 0 
            (0 0) 1 2 3 0 180) ""   1559..1786"" 1))) ""IR/DR(R)""))) 
            ""Transposon (2 total)"") #185=(CFolderPar (CGroupPar (CParagraph 87
            (7 87 0) 1 1 1 0 178) (CObjectList                           
            #186=(CFolderPar (CGroupPar (CParagraph 1222 (3 #94# 0) 1 2 2 0 194)
            (CObjectList #187=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
            ""Start: 1222  End: 1256"" 1))) ""Lck Myr/Palm""))) ""Misc. Signal 
            (1 total)""))) ""Feature Map"")           #188=(CFolderPar 
            (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) (CObjectList 
            #34=(CRSite (CStringList) ""EaeI"" ""yggccr"" 2 0 7 1363 0 1417 0 
            1709 0          1856 0 2274 0 2720 0 2860 0 0 """") #35=(CRSite 
            (CStringList) ""EagI"" ""cggccg"" 2 0 2 1417 0 2274 0 0 """") 
            #36=(CRSite (CStringList) ""EcoRI"" ""gaattc"" 2 0 1 2796 0 0 """") 
            #37=(CRSite (CStringList) ""EcoRV"" ""gatatc"" 4 0 0 0 """") 
            #38=(CRSite (CStringList) ""HindII"" ""gtyrac"" 4 0 4 100 0 250 0 
            478 0 2994 0 0 """")     #39=(CRSite (CStringList) ""HindIII"" 
            ""aagctt"" 2 0 2 1028 0 2362 0 0 """")     #40=(CRSite (CStringList)
            ""HinP1I"" ""gcgc"" 2 0 16 1468 0 2057 0 2403 0          2688 0 2817
            0 2819 0 3442 0 3712 0 3779 0 3879 0 4053 0 4162 0 4685          0 
            4755 0 4772 0 4994 0 0 """")     #41=(CRSite (CStringList) ""HpaI"" 
            ""gttaac"" 4 0 2 100 0 2994 0 0 """")     #42=(CRSite (CStringList) 
            ""KasI"" ""ggcgcc"" 2 0 0 0 """") #43=(CRSite (CStringList) ""KpnI""
            ""ggtacc"" 6 0 2 2844 0 3402 0 0 """") #44=(CRSite (CStringList) 
            ""MfeI"" ""caattg"" 2 0 4 169 0 899 0 1161 0 3003          0 0 """")
            #45=(CRSite (CStringList) ""MluI"" ""acgcgt"" 2 0 0 0 """") 
            #46=(CRSite (CStringList) ""MscI"" ""tggcca"" 4 0 3 1711 0 1858 0 
            2862 0 0          0 2840 0 3398 0 0 """")     #21=(CRSite 
            (CStringList) ""BanII"" ""grgcyc"" 6 0 6 1666 0 2046 0 2375 0 2482 0
            2851 0 5058 0 0 """")     #22=(CRSite (CStringList) ""BbsI"" 
            ""gaagac"" 9 13 2 1492 0 2221 0 0 """")     #23=(CRSite 
            (CStringList) ""BclI"" ""tgatca"" 2 0 1 2633 0 0 """")     
            #24=(CRSite (CStringList) ""BglI"" ""gccnnnnnggc"" 8 0 0 0 """")    
            #25=(CRSite (CStringList) ""BglII"" ""agatct"" 2 0 1 2833 0 0 """") 
               #26=(CRSite (CStringList) ""BsaBI"" ""gatnnnnatc"" 6 0 2 9 0 2638
            0 0 """")     #27=(CRSite (CStringList) ""BsiWI"" ""cgtacg"" 2 0 1 
            2810 0 0 """")     #28=(CRSite (CStringList) ""BspHI"" ""tcatga"" 2 
            0 2 4254 0 5181 0 0 """") #29=(CRSite (CStringList) ""BsrGI"" 
            ""tgtaca"" 2 0 1 1204 0 0 """") #30=(CRSite (CStringList) ""BstXI"" 
            ""ccannnnnntgg"" 9 0 1 2858 0 0 """") #31=(CRSite (CStringList) 
            ""ClaI"" ""atcgat"" 3 0 0 0 """") #32=(CRSite (CStringList) ""DpnI""
            ""gatc"" 3 0 22 6 0 12 0 318 0 1789 0          1834 0 2164 0 2194 0 
            2392 0 2500 0 2635 0 2835 0 3274 0 3394 0 4102          0 4177 0 
            4188 0 4196 0 4274 0 4286 0 4458 0 4710 0 4840 0 0 """") #33=(CRSite
            (CStringList) ""DraIII"" ""cacnnngtg"" 7 0 2 1672 0 1728 0 0 """")  
                   0 4863 0 0 """")     #57=(CRSite (CStringList) ""NspI"" 
            ""rcatgy"" 6 0 3 597 0 822 0 3538 0 0 """") #58=(CRSite 
            (CStringList) ""PacI"" ""ttaattaa"" 6 0 0 0 """") #59=(CRSite 
            (CStringList) ""PmeI"" ""gtttaaac"" 5 0 1 336 0 0 """") #60=(CRSite 
            (CStringList) ""PstI"" ""ctgcag"" 6 0 4 379 0 1140 0 1532 0 1904 0 0
            """")     #61=(CRSite (CStringList) ""PstII"" ""ctgatg"" 32 34 2 
            1510 0 4837 1 0 """")     #62=(CRSite (CStringList) ""PvuI"" 
            ""cgatcg"" 5 0 1 4711 0 0 """")     #63=(CRSite (CStringList) 
            ""PvuII"" ""cagctg"" 4 0 2 1 0 1479 0 0 """")     #64=(CRSite 
            (CStringList) ""SacI"" ""gagctc"" 6 0 1 2375 0 0 """")     
            #65=(CRSite (CStringList) ""SacII"" ""ccgcgg"" 5 0 0 0 """")     
            #66=(CRSite (CStringList) ""SalI"" ""gtcgac"" 2 0 0 0 """") 
            #67=(CRSite (CStringList) ""SapI"" ""gctcttc"" 9 12 1 808 1 0 """") 
            #68=(CRSite (CStringList) ""SbfI"" ""cctgcagg"" 7 0 1 379 0 0 """") 
            #69=(CRSite (CStringList) ""ScaI"" ""agtact"" 4 0 1 1939 0 0 """") 
            #70=(CRSite (CStringList) ""SfiI"" ""ggccnnnnnggcc"" 9 0 0 0 """") 
            #71=(CRSite (CStringList) ""SgrAI"" ""crccggyg"" 3 0 0 0 """") """")
            #47=(CRSite (CStringList) ""MspI"" ""ccgg"" 2 0 15 321 0 353 0 1500 
            0 1656 0          2016 0 2040 0 2112 0 2522 0 3741 0 3888 0 3914 0 
            4104 0 4571 0 4752          0 4834 0 0 """")     #48=(CRSite 
            (CStringList) ""NaeI"" ""gccggc"" 4 0 0 0 """")     #49=(CRSite 
            (CStringList) ""NarI"" ""ggcgcc"" 3 0 0 0 """")     #50=(CRSite 
            (CStringList) ""NcoI"" ""ccatgg"" 2 0 1 1220 0 0 """")     
            #51=(CRSite (CStringList) ""NdeI"" ""catatg"" 3 0 1 1295 0 0 """")  
              #52=(CRSite (CStringList) ""NheI"" ""gctagc"" 2 0 2 361 0 2854 0 0
            """") #53=(CRSite (CStringList) ""NlaII"" ""gatc"" 1 0 22 4 0 10 0 
            316 0 1787 0 1832 0 2162 0 2192 0 2390 0 2498 0 2633 0 2833 0 3272 0
            3392 0 4100 0 4175 0 4186 0 4194 0 4272 0 4284 0 4456 0 4708 0 4838 
            0 0 """") #54=(CRSite (CStringList) ""NlaIII"" ""catg"" 5 0 24 149 0
            597 0 822 0 862 0          1224 0 1564 0 1737 0 1830 0 1977 0 2139 0
            2214 0 2313 0 2359 0 2385          0 2505 0 2670 0 2871 0 3538 0 
            4258 0 4556 0 4861 0 4976 0 5088 0          5185 0 0 """")     
            #55=(CRSite (CStringList) ""NotI"" ""gcggccgc"" 3 0 1 1417 0 0 """")
                #56=(CRSite (CStringList) ""NsiI"" ""atgcat"" 6 0 5 350 0 1154 0
            2829 0 4597 (CTerm 1 1 0 ""NcoI"" """" 1 0 0 0 0 0 0 0 0 0) 1 0)    
             #1=(CFragment 3 1 0 808525932 0 """" 0 """" """" """" """" """" 
            """" ""Litmus wtAKT""          """" 0 0 1 1613 1220 2832 2787 37 0 
            """" """" """" """" """" """" (CObList) (CTerm 1 0 0 ""NcoI"" """" 1
            0 0 0 0 0 0 0 0 0)          (CTerm 1 1 0 ""BglII"" """" 1 0 0 0 0 0 
            0 0 0 0) 1 0)) (CObList) -1 """") (CDocSetData 1 1 0 0 0 2 ""MAIN"" 
            1 1 1 1 0 0 1 1 0 1 10 10 4294967295 150     0 1 0 (CHomObj 0 0 0 3 
            75) (CWordArray) (CWordArray)     (CStringList ""AatII"" ""AciI"" 
            ""AflII"" ""AflIII"" ""AgeI"" ""AluI"" ""ApaI""      ""ApaLI"" 
            ""ApoI"" ""AscI"" ""AseI"" ""AseII"" ""attL2/R2 recombinase""      
            ""attR1/L1 recombinase"" ""AvaI"" ""AvaII"" ""AvrII"" ""BamHI"" 
            ""BanI"" ""BanII""      ""BbsI"" ""BclI"" ""BglI"" ""BglII"" 
            ""BsaBI"" ""BsiWI"" ""BspHI"" ""BsrGI"" ""BstXI""      ""ClaI"" 
            ""DpnI"" ""DraIII"" ""EaeI"" ""EagI"" ""EcoRI"" ""EcoRV"" ""HindII""
            ""HindIII"" ""HinP1I"" ""HpaI"" ""KasI"" ""KpnI"" ""MfeI"" ""MluI"" 
            ""MscI"" ""MspI"" ""NaeI"" ""NarI""      ""NcoI"" ""NdeI"" ""NheI"" 
            ""NlaII"" ""NlaIII"" ""NotI"" ""NsiI"" ""NspI"" ""PacI"" ""PmeI"" 
            ""PstI"" ""PstII"" ""PvuI"" ""PvuII"" ""SacI"" ""SacII"" ""SalI"" 
            ""SapI"" ""SbfI"" ""ScaI"" ""SfiI"" ""SgrAI"" ""SmaI"" ""SnaBI"" 
            ""SpeI"" ""SphI"" ""SspI"" ""StuI""      ""StyI"" ""SwaI"" ""XbaI"" 
            ""XcmI"" ""XhoI"" ""XmaI"" ""XmnI"")
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(20..246)
                     /label=IR /DR
                     /label=nonstandard type: transposon
                     /note="/dnas_title=IR"
                     /note="/vntifkey=IR"
     enhancer        380..802
                     /label=mCMV enhancer
                     /note="/dnas_title=mCMV enhancer"
                     /note="/vntifkey=CMV/enhancer"
     promoter        803..1026
                     /label=hEF1alpha promoter
                     /note="/dnas_title=hEF1alpha promoter"
                     /note="/vntifkey=EF1 promoter"
     intron          1058..1197
                     /label=I 140
                     /note="/dnas_title=I 140"
                     /note="/vntifkey=intron"
     misc_feature    1209..2466
                     /label=IDH1-R132H
     misc_feature    2473..3047
                     /label=IRES
     gene            3048..3767
                     /label=katushka
                     /note="katushka"
     polyA_signal    3821..4046
                     /label=SV40 polyA-n
                     /note="/dnas_title=SV40 polyA-n"
                     /note="/vntifkey=polyA"
     misc_feature    4117..4344
                     /label=IR /DR
                     /label=IR /DR(1)
                     /label=nonstandard type: transposon
                     /note="/dnas_title=IR"
                     /note="/vntifkey=IR"
     rep_origin      4370..5251
                     /label=ColE1 Ori
                     /note="/dnas_title=ColE1 Ori"
                     /note="/vntifkey=Ori"
     CDS             complement(5284..6093)
                     /label=Kan(R)
                     /note="/dnas_title=Kan(R)"
                     /note="/vntifkey=Kan (R)"
ORIGIN
        1 ctggatccag atccctatac agttgaagtc ggaagtttac atacacttaa gttggagtca
       61 ttaaaactcg tttttcaact actccacaaa tttcttgtta acaaacaata gttttggcaa
      121 gtcagttagg acatctactt tgtgcatgac acaagtcatt tttccaacaa ttgtttacag
      181 acagattatt tcacttataa ttcactgtat cacaattcca gtgggtcaga agtttacata
      241 cactaagttg actgtgcctt taaacagctt ggaaaattcc agaaaatgat ggtctcccta
      301 tagtgagtcg tattagatcc cgggattact agtttaaacc taggatgcat accggtcaag
      361 ctagcgataa ttcctgcagg agtcaatggg aaaaacccat tggagccaag tacactgact
      421 caatagggac tttccattgg gttttgccca gtacataagg tcaatagggg gtgagtcaac
      481 aggaaagtcc cattggagcc aagtacattg agtcaatagg gactttccaa tgggttttgc
      541 ccagtacata aggtcaatgg gaggtaagcc aatgggtttt tcccattact gacatgtata
      601 ctgagtcatt agggactttc caatgggttt tgcccagtac ataaggtcaa taggggtgaa
      661 tcaacaggaa agtcccattg gagccaagta cactgagtca atagggactt tccattgggt
      721 tttgcccagt acaaaaggtc aatagggggt gagtcaatgg gtttttccca ttattggcac
      781 atacataagg tcaatagggg tgactagtgg agaagagcat gcttgagggc tgagtgcccc
      841 tcagtgggca gagagcacat ggcccacagt ccctgagaag ttggggggag gggtgggcaa
      901 ttgaactggt gcctagagaa ggtggggctt gggtaaactg ggaaagtgat gtggtgtact
      961 ggctccacct ttttccccag ggtgggggag aaccatatat aagtgcagta gtctctgtga
     1021 acattcaagc ttctgccttc tccctcctgt gagtttggta agtcactgac tgtctatgcc
     1081 tgggaaaggg tgggcaggag atggggcagt gcaggaaaag tggcactatg aaccctgcag
     1141 ccctaggaat gcatctagac aattgtacta accttcttct ctttcctctc ctgacaggtt
     1201 ggtgtacagc ggcccgccat gtccaaaaaa atcagtggcg gttctgtggt agagatgcaa
     1261 ggagatgaaa tgacacgaat catttgggaa ttgattaaag agaaactcat ttttccctac
     1321 gtggaattgg atctacatag ctatgattta ggcatagaga atcgtgatgc caccaacgac
     1381 caagtcacca aggatgctgc agaagctata aagaagcata atgttggcgt caaatgtgcc
     1441 actatcactc ctgatgagaa gagggttgag gagttcaagt tgaaacaaat gtggaaatca
     1501 ccaaatggca ccatacgaaa tattctgggt ggcacggtct tcagagaagc cattatctgc
     1561 aaaaatatcc cccggcttgt gagtggatgg gtaaaaccta tcatcatagg tcatcatgct
     1621 tatggggatc aatacagagc aactgatttt gttgttcctg ggcctggaaa agtagagata
     1681 acctacacac caagtgacgg aacccaaaag gtgacatacc tggtacataa ctttgaagaa
     1741 ggtggtggtg ttgccatggg gatgtataat caagataagt caattgaaga ttttgcacac
     1801 agttccttcc aaatggctct gtctaagggt tggcctttgt atctgagcac caaaaacact
     1861 attctgaaga aatatgatgg gcgttttaaa gacatctttc aggagatata tgacaagcag
     1921 tacaagtccc agtttgaagc tcaaaagatc tggtatgagc ataggctcat cgacgacatg
     1981 gtggcccaag ctatgaaatc agagggaggc ttcatctggg cctgtaaaaa ctatgatggt
     2041 gacgtgcagt cggactctgt ggcccaaggg tatggctctc tcggcatgat gaccagcgtg
     2101 ctggtttgtc cagatggcaa gacagtagaa gcagaggctg cccacgggac tgtaacccgt
     2161 cactaccgca tgtaccagaa aggacaggag acgtccacca atcccattgc ttccattttt
     2221 gcctggacca gagggttagc ccacagagca aagcttgata acaataaaga gcttgccttc
     2281 tttgcaaatg ctttggaaga agtctctatt gagacaattg aggctggctt catgaccaag
     2341 gacttggctg cttgcattaa aggtttaccc aatgtgcaac gttctgacta cttgaataca
     2401 tttgagttca tggataaact tggagaaaac ttgaagatca aactagctca ggccaaactt
     2461 acgcgtctcg aggcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     2521 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     2581 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     2641 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     2701 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     2761 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     2821 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctcacctcaa
     2881 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     2941 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc
     3001 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gtgggtgagg
     3061 atagcgtgct gatcaccgag aacatgcaca tgaaactgta catggagggc accgtgaacg
     3121 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     3181 tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
     3241 gcttcatgta cggcagcaaa acctttatca accacaccca gggcatcccc gacttcttta
     3301 agcagtcctt ccctgagggc ttcacatggg agaggatcac cacatacgaa gacgggggcg
     3361 tgctgaccgc tacccaggac accagcctcc agaacggctg cctcatctac aacgtcaaga
     3421 tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     3481 aggccagcac cgagatgctg taccccgctg acagcggcct gagaggccat agccagatgg
     3541 ccctgaagct cgtgggcggg ggctacctgc actgctccct caagaccaca tacagatcca
     3601 agaaacccgc taagaacctc aagatgcccg gcttctactt cgtggacagg agactggaaa
     3661 gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgagatggct gtggccaggt
     3721 actgcgacct gcctagcaaa ctggggcaca gctccggact cagataacgt acgtgcgcgc
     3781 gaatgcatcc agatctaggt accagggccc agctagctgg ccagacatga taagatacat
     3841 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     3901 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     3961 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     4021 gtaaaacctc tacaaatgtg gtatggaatt atcaattaac cctcactaaa gggagaccaa
     4081 gttaaacaat ttaaaggcaa tgctaccaaa tactaattga gtgtatgtaa acttctgacc
     4141 cactgggaat gtgatgaaag aaataaaagc tgaaatgaat cattctctct actattattc
     4201 tgatatttca cattcttaaa ataaagtggt gatcctaact gacctaagac agggaatttt
     4261 tactaggatt aaatgtcagg aattgtgaaa aagtgagttt aaatgtattt ggctaaggtg
     4321 tatgtaaact tccgacttca actgtatagg gatctggtac catttaaatc tgttccgctt
     4381 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4441 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4501 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4561 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4621 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4681 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4741 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4801 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4861 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4921 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4981 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5041 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5101 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5161 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5221 tatcaaaaag gatcttcacc tagatccttt ttgccagtgt tacaaccaat taaccaattc
     5281 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     5341 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     5401 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     5461 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     5521 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     5581 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     5641 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     5701 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     5761 atattcttct aatacctgga atgctgtttt tccggggatc gcagtggtga gtaaccatgc
     5821 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     5881 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     5941 aaacaactct ggcgcatcgg gcttcccata caagcgatag attgtcgcac ctgattgccc
     6001 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     6061 cggcctcgac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
     6121 agcagacagt tttattgttc atgatgcag
//
