LOCUS       Exported                7100 bp ds-DNA     circular SYN 04-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 26, 2019 from SnapGene Viewer 4.3.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    209..863
                     /label=CMV promoter
     misc_feature    864..882
                     /label=T7
     primer_bind     938..973
                     /label=Fragment.FOR
     primer_bind     complement(942..977)
                     /label=Vector.REV
     misc_feature    944..951
     misc_feature    953..960
                     /label=pCDNA3 Jmjd2d
     CDS             958..2094
                     /codon_start=1
                     /gene="NET23"
                     /label=NET23
                     /translation="MPHSSLHPSIPCPRGHGAQKAALVLLSACLVTLWGLGEPPEHTLR
                     YLVLHLASLQLGLLLNGVCSLAEELRHIHSRYRGSYWRTVRACLGCPLRRGALLLLSIY
                     FYYSLPNAVGPPFTWMLALLGLSQALNILLGLKGLAPAEISAVCEKGNFNVAHGLAWSY
                     YIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFL
                     DKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSR
                     EDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEE
                     VTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS"
     CDS             958..960
                     /label=p65 CDS
     primer_bind     2075..2118
                     /label=Vector.FOR
     primer_bind     complement(2075..2114)
                     /label=Fragment.REV
     misc_feature    2095..2618
                     /label=pCDNA3 Jmjd2d
     CDS             2095..2106
                     /label=linking peptide
     misc_feature    2096..2618
                     /label=p123T+p65-biotin tag
                     /note="LOCUS ASP123T 3003 bp DNA SYN 17-FEB-1997DEFINITION 
                     Artificial DNA for p123T PCR cloning vector. ACCESSION 
                     Z46733NID g576935KEYWORDS cloning vector.SOUR CE synthetic 
                     construct. ORGANISM synthetic construct artificial 
                     sequence.REFERENCE 1 (bases 1 to 3003) AUTHORS M 
                     itchell,D.B., Ruggli,N. and Tratschin,J.D. TITLE An 
                     improved me thod for cloning PCR fragments JOURNAL PCR 
                     Methods Appl. 2 (1), 8 1-82 (1992) MEDLINE 
                     93145058REFERENCE 2 (bases 1 to 3003) AUT HORS 
                     Mitchell,D.B. TITLE Direct Submission JOURNAL Submitt ed 
                     (21-NOV-1994) David B Mitchell, Institute fuer Viruskr 
                     ankheiten und Immunprophylaxe, Mittelhaeusern, CH-3147, S 
                     witzerlandFEATURES Location/Qualifiers source 1..3003 
                     /organism=""synthetic construct"" /db_xref=""taxon:32630"" 
                     /clone =""p123T"" misc_feature join(1..712,761..3003) 
                     /note=""pBluescript II SK (+) phagemid backbone"" misc_fea 
                     ture 713..760 /note=""3T cloning site"" m utation 2561 
                     /note=""mutation to remove n atural Bcg I site"" 
                     /replace=""c"" mutation 2563 /note=""mutation to remove 
                     natural B cg I site"" /replace=""t""BASE COUNT 756 a 751 c 
                     762 g 734 tORIGIN"
     CDS             2107..2142
                     /label=GGGGSGGGGSSG linker
     CDS             2143..2616
                     /label=Venus fragment 1
     misc_feature    2653..2670
                     /label=Sp6
     misc_feature    2672..2903
                     /label=BGH polyA
     misc_feature    3444..3769
                     /label=SV40 promoter
     misc_feature    3638..3723
                     /label=SV40 ori
     CDS             3805..4599
                     /label=neo
     misc_feature    4654..5026
                     /label=SV40 polyA
     misc_feature    5286..5959
                     /label=ColE1 origin
     CDS             complement(6104..6964)
                     /label=bla
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcctgcagg cctcgagatg
      961 ccccactcca gcctgcatcc atccatcccg tgtcccaggg gtcacggggc ccagaaggca
     1021 gccttggttc tgctgagtgc ctgcctggtg accctttggg ggctaggaga gccaccagag
     1081 cacactctcc ggtacctggt gctccaccta gcctccctgc agctgggact gctgttaaac
     1141 ggggtctgca gcctggctga ggagctgcgc cacatccact ccaggtaccg gggcagctac
     1201 tggaggactg tgcgggcctg cctgggctgc cccctccgcc gtggggccct gttgctgctg
     1261 tccatctatt tctactactc cctcccaaat gcggtcggcc cgcccttcac ttggatgctt
     1321 gccctcctgg gcctctcgca ggcactgaac atcctcctgg gcctcaaggg cctggcccca
     1381 gctgagatct ctgcagtgtg tgaaaaaggg aatttcaacg tggcccatgg gctggcatgg
     1441 tcatattaca tcggatatct gcggctgatc ctgccagagc tccaggcccg gattcgaact
     1501 tacaatcagc attacaacaa cctgctacgg ggtgcagtga gccagcggct gtatattctc
     1561 ctcccattgg actgtggggt gcctgataac ctgagtatgg ctgaccccaa cattcgcttc
     1621 ctggataaac tgccccagca gaccggtgac catgctggca tcaaggatcg ggtttacagc
     1681 aacagcatct atgagcttct ggagaacggg cagcgggcgg gcacctgtgt cctggagtac
     1741 gccaccccct tgcagacttt gtttgccatg tcacaataca gtcaagctgg ctttagccgg
     1801 gaggataggc ttgagcaggc caaactcttc tgccggacac ttgaggacat cctggcagat
     1861 gcccctgagt ctcagaacaa ctgccgcctc attgcctacc aggaacctgc agatgacagc
     1921 agcttctcgc tgtcccagga ggttctccgg cacctgcggc aggaggaaaa ggaagaggtt
     1981 actgtgggca gcttgaagac ctcagcggtg cccagtacct ccacgatgtc ccaagagcct
     2041 gagctcctca tcagtggaat ggaaaagccc ctccctctcc gcacggattt ctcttctaga
     2101 ggatccggtg gcggtggctc tggaggtggt gggtcctccg gagtgagcaa gggcgaggag
     2161 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2221 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
     2281 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac
     2341 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2401 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2461 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2521 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     2581 agccacaacg tctatatcac cgccgacaag cagtaagcgg ccgcgtcgag catgcatcta
     2641 gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg
     2701 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     2761 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     2821 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     2881 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     2941 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3001 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3061 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3121 ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3181 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3241 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3301 atctcggtct attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa
     3361 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     3421 ggtgtggaaa gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat
     3481 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3541 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3601 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3661 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3721 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     3781 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     3841 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     3901 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     3961 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     4021 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4081 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4141 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4201 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4261 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4321 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4381 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4441 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4501 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4561 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     4621 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     4681 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     4741 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     4801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4861 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     4921 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4981 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5041 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5101 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     5161 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5221 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5281 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5521 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     5581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5761 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     5821 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5881 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5941 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6001 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6061 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6241 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6661 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6721 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6781 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6841 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6901 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6961 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7021 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7081 gaaaagtgcc acctgacgtc
//
