LOCUS       Exported                6872 bp ds-DNA     circular SYN 04-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 26, 2019 from SnapGene Viewer 4.3.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    209..863
                     /label=CMV promoter
     misc_feature    864..882
                     /label=T7
     primer_bind     938..973
                     /label=Fragment.FOR
     primer_bind     complement(942..977)
                     /label=Vector.REV
     misc_feature    944..951
     misc_feature    953..960
                     /label=Construct: pCDNA3 p65-Venus1
                     /label=Construct: pCDNA3 p65-Venus1
     CDS             958..2094
                     /codon_start=1
                     /gene="NET23"
                     /label=NET23
                     /translation="MPHSSLHPSIPCPRGHGAQKAALVLLSACLVTLWGLGEPPEHTLR
                     YLVLHLASLQLGLLLNGVCSLAEELRHIHSRYRGSYWRTVRACLGCPLRRGALLLLSIY
                     FYYSLPNAVGPPFTWMLALLGLSQALNILLGLKGLAPAEISAVCEKGNFNVAHGLAWSY
                     YIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFL
                     DKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSR
                     EDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEE
                     VTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS"
     CDS             958..960
                     /label=p65 CDS
     primer_bind     2075..2118
                     /label=Vector.FOR
     primer_bind     complement(2075..2114)
                     /label=Fragment.REV
     misc_feature    2095..2390
                     /label=Construct: pCDNA3 p65-Venus1
                     /label=Construct: pCDNA3 p65-Venus1
     CDS             2095..2106
                     /label=linking peptide
     misc_feature    2096..2390
                     /label=Construct: p123T+p65-Venus1
                     /label=Construct: p123T+p65-Venus1
                     /note="LOCUS ASP123T 3003 bp DNA SYN 17-FEB-1997DEFINITION 
                     Artificial DNA for p123T PCR cloning vector. ACCESSION 
                     Z46733NID g576935KEYWORDS cloning vector.SOUR CE synthetic 
                     construct. ORGANISM synthetic construct artificial 
                     sequence.REFERENCE 1 (bases 1 to 3003) AUTHORS M 
                     itchell,D.B., Ruggli,N. and Tratschin,J.D. TITLE An 
                     improved me thod for cloning PCR fragments JOURNAL PCR 
                     Methods Appl. 2 (1), 8 1-82 (1992) MEDLINE 
                     93145058REFERENCE 2 (bases 1 to 3003) AUT HORS 
                     Mitchell,D.B. TITLE Direct Submission JOURNAL Submitt ed 
                     (21-NOV-1994) David B Mitchell, Institute fuer Viruskr 
                     ankheiten und Immunprophylaxe, Mittelhaeusern, CH-3147, S 
                     witzerlandFEATURES Location/Qualifiers source 1..3003 
                     /organism=""synthetic construct"" /db_xref=""taxon:32630"" 
                     /clone =""p123T"" misc_feature join(1..712,761..3003) 
                     /note=""pBluescript II SK (+) phagemid backbone"" misc_fea 
                     ture 713..760 /note=""3T cloning site"" m utation 2561 
                     /note=""mutation to remove n atural Bcg I site"" 
                     /replace=""c"" mutation 2563 /note=""mutation to remove 
                     natural B cg I site"" /replace=""t""BASE COUNT 756 a 751 c 
                     762 g 734 tORIGIN"
     CDS             2107..2142
                     /label=GGGGSGGGGSSG linker
     CDS             2143..2388
                     /label=Venus frament 2
     misc_feature    2425..2442
                     /label=Sp6
     misc_feature    2444..2675
                     /label=BGH polyA
     misc_feature    3216..3541
                     /label=SV40 promoter
     misc_feature    3410..3495
                     /label=SV40 ori
     CDS             3577..4371
                     /label=neo
     misc_feature    4426..4798
                     /label=SV40 polyA
     misc_feature    5058..5731
                     /label=ColE1 origin
     CDS             complement(5876..6736)
                     /label=bla
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcctgcagg cctcgagatg
      961 ccccactcca gcctgcatcc atccatcccg tgtcccaggg gtcacggggc ccagaaggca
     1021 gccttggttc tgctgagtgc ctgcctggtg accctttggg ggctaggaga gccaccagag
     1081 cacactctcc ggtacctggt gctccaccta gcctccctgc agctgggact gctgttaaac
     1141 ggggtctgca gcctggctga ggagctgcgc cacatccact ccaggtaccg gggcagctac
     1201 tggaggactg tgcgggcctg cctgggctgc cccctccgcc gtggggccct gttgctgctg
     1261 tccatctatt tctactactc cctcccaaat gcggtcggcc cgcccttcac ttggatgctt
     1321 gccctcctgg gcctctcgca ggcactgaac atcctcctgg gcctcaaggg cctggcccca
     1381 gctgagatct ctgcagtgtg tgaaaaaggg aatttcaacg tggcccatgg gctggcatgg
     1441 tcatattaca tcggatatct gcggctgatc ctgccagagc tccaggcccg gattcgaact
     1501 tacaatcagc attacaacaa cctgctacgg ggtgcagtga gccagcggct gtatattctc
     1561 ctcccattgg actgtggggt gcctgataac ctgagtatgg ctgaccccaa cattcgcttc
     1621 ctggataaac tgccccagca gaccggtgac catgctggca tcaaggatcg ggtttacagc
     1681 aacagcatct atgagcttct ggagaacggg cagcgggcgg gcacctgtgt cctggagtac
     1741 gccaccccct tgcagacttt gtttgccatg tcacaataca gtcaagctgg ctttagccgg
     1801 gaggataggc ttgagcaggc caaactcttc tgccggacac ttgaggacat cctggcagat
     1861 gcccctgagt ctcagaacaa ctgccgcctc attgcctacc aggaacctgc agatgacagc
     1921 agcttctcgc tgtcccagga ggttctccgg cacctgcggc aggaggaaaa ggaagaggtt
     1981 actgtgggca gcttgaagac ctcagcggtg cccagtacct ccacgatgtc ccaagagcct
     2041 gagctcctca tcagtggaat ggaaaagccc ctccctctcc gcacggattt ctcttctaga
     2101 ggatccggtg gcggtggctc tggaggtggt gggtcctccg gaaagaacgg catcaaggcc
     2161 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag
     2221 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     2281 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2341 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc ggccgcgtcg
     2401 agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca
     2461 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     2521 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     2581 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     2641 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     2701 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     2761 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2821 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2881 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2941 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3001 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3061 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc
     3121 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     3181 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
     3241 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3301 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     3361 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3421 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     3481 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     3541 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     3601 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     3661 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     3721 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     3781 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     3841 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     3901 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     3961 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     4021 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     4081 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     4141 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     4201 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     4261 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     4321 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     4381 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     4441 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     4501 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     4561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     4681 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     4741 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     4801 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     4861 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4921 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4981 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5041 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5221 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5281 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     5341 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5401 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5461 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5521 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5581 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5641 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5701 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5761 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5821 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5881 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5941 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6001 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6061 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6121 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6181 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6241 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6301 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6361 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6421 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6481 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6541 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6601 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6661 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6721 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6781 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6841 gcacatttcc ccgaaaagtg ccacctgacg tc
//
