LOCUS       Exported                5112 bp ds-DNA     circular SYN 04-MAY-2017
DEFINITION  (unc-119 shortened rescue fragment; 
            unc-14pro::his-24::wormCherry::let-858 3'UTR; amp resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 1, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2166..2899
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
     misc_feature    691..696
                     /label=Post Promoter
     misc_feature    697..1341
                     /label=his-24 CDS
                     /label=his-24\CDS
     misc_feature    1348..2151
                     /label=tagBFP2
     misc_feature    2166..2899
                     /label=unc-54 3' UTR
                     /note="unc-54 3' UTR"
     misc_feature    2183..2208
                     /label=Z21 rev seq primer
                     /note="Z21 rev seq primer"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggacta gtggatcccc cgggctgcag cctaggatgt ctgattccgc tgttgttgcc
      721 gccgctgtcg agccaaaggt cccaaaggct aaggccgcca aggccgccaa gccagccgcc
      781 aaggccgcca agccaaccaa agttgccaag gccaaggctc cagtcgctca cccaccatac
      841 atcaacatga tcaaggaggc catcaagcag ctcaaggacc gcaaaggagc ttccaagcag
      901 gcaatcctca agttcatctc ccaaaactac aagctcggag acaatgtcat ccagatcaat
      961 gctcatctcc gtcaggctct caagcgtggt gtcaccagca aggcccttgt tcaagccgcc
     1021 ggatccggag ccaacggacg tttccgtgtg ccagagaagg ccgccgccgc caagaagcca
     1081 gcagcagcca agaagccagc agcagccaag aaaccagcag ccgccaagaa agccactgga
     1141 gagaagaagg caaagaagcc agccgctgcc aagccaaaga aggccgccac cggagataag
     1201 aaggtcaaga aggccaagag cccaaagaag gttgccaagc cagctgccaa gaaggtcgcc
     1261 aagtctccag ccaagaaggc cgctccaaag aagatcgcca agccagctgc caagaaggcc
     1321 gccaagccag ccgccaaggc cgcatgcatg tccgaactca tcaaggagaa catgcacatg
     1381 aagctctaca tggagggaac cgtagacaac caccacttca agtgcacctc cgagggagag
     1441 ggaaagccat acgagggaac ccaaaccatg cgtatcaagg tcgtcgaggg aggaccactc
     1501 ccattcgcct tcgacatcct cgccacctcc ttcctctacg ggtccaaggt aagtttaaac
     1561 atatatatac taactaaccc tgattattta aattttcaga ccttcatcaa ccacacccaa
     1621 ggaatcccag acttcttcaa gcaatccttc ccagagggat tcacctggga gcgtgtcacc
     1681 acctacgagg acggaggagt cctcaccgcc acccaagaca cctccctcca agacggatgc
     1741 ctcatctaca acgtcaagat ccgtggagtc aacttcacct ccaacggacc agtcatgcaa
     1801 aagaagaccc tcggatggga gaccttcacc gagaccctct acccagccga cggaggacta
     1861 gagggacgta acgacatggc cctcaagctc gtcggaggct cccacctcat cgccaacgcc
     1921 aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagaccacc
     1981 taccgttcca agaagccagc caagaacctc aagatgccag gagtctacta cgttgactac
     2041 cgtctagagc gtatcaagga ggccaacaac gagacctacg tcgagcaaca cgaggtcgcc
     2101 gtcgcccgtt actgcgacct cccatccaag ctcggacaca agctcaacta aaagctttgg
     2161 aattcatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca
     2221 tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa acttcttctt
     2281 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca
     2341 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat
     2401 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttttact
     2461 tctgataaat tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     2521 gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac
     2581 gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg
     2641 aaagtttatg aaattaattt tcctgctttt gctttttggg ggtttcccct attgtttgtc
     2701 aagagtttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca
     2761 agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa
     2821 tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat
     2881 ataccaaaca taactgtttg ggcccggtac ccagcttttg ttccctttag tgagggttaa
     2941 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     3001 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3061 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3121 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3181 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3241 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3301 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3361 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3421 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3481 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3541 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3601 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3661 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3721 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3781 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     3841 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3901 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3961 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4021 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4081 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4141 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4201 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4261 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4321 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4381 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4441 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4501 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4561 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4621 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4681 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4741 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4801 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4861 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4921 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4981 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5041 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5101 gaaaagtgcc ac
//
