LOCUS       Exported                4541 bp ds-DNA     circular SYN 04-MAY-2017
DEFINITION  (unc-119 shortened rescue fragment; 
            unc-14pro::his-24::wormCherry::let-858 3'UTR; amp resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 1, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    691..696
                     /label=Post Promoter
     misc_feature    697..1341
                     /label=his-24 CDS
                     /label=his-24\CDS
     misc_feature    1348..2151
                     /label=tagBFP2
     3'UTR           2166..2328
                     /label=let-858 3'UTR
                     /label=let-858\3'UTR
                     /note="/vntifkey=50"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggacta gtggatcccc cgggctgcag cctaggatgt ctgattccgc tgttgttgcc
      721 gccgctgtcg agccaaaggt cccaaaggct aaggccgcca aggccgccaa gccagccgcc
      781 aaggccgcca agccaaccaa agttgccaag gccaaggctc cagtcgctca cccaccatac
      841 atcaacatga tcaaggaggc catcaagcag ctcaaggacc gcaaaggagc ttccaagcag
      901 gcaatcctca agttcatctc ccaaaactac aagctcggag acaatgtcat ccagatcaat
      961 gctcatctcc gtcaggctct caagcgtggt gtcaccagca aggcccttgt tcaagccgcc
     1021 ggatccggag ccaacggacg tttccgtgtg ccagagaagg ccgccgccgc caagaagcca
     1081 gcagcagcca agaagccagc agcagccaag aaaccagcag ccgccaagaa agccactgga
     1141 gagaagaagg caaagaagcc agccgctgcc aagccaaaga aggccgccac cggagataag
     1201 aaggtcaaga aggccaagag cccaaagaag gttgccaagc cagctgccaa gaaggtcgcc
     1261 aagtctccag ccaagaaggc cgctccaaag aagatcgcca agccagctgc caagaaggcc
     1321 gccaagccag ccgccaaggc cgcatgcatg tccgaactca tcaaggagaa catgcacatg
     1381 aagctctaca tggagggaac cgtagacaac caccacttca agtgcacctc cgagggagag
     1441 ggaaagccat acgagggaac ccaaaccatg cgtatcaagg tcgtcgaggg aggaccactc
     1501 ccattcgcct tcgacatcct cgccacctcc ttcctctacg ggtccaaggt aagtttaaac
     1561 atatatatac taactaaccc tgattattta aattttcaga ccttcatcaa ccacacccaa
     1621 ggaatcccag acttcttcaa gcaatccttc ccagagggat tcacctggga gcgtgtcacc
     1681 acctacgagg acggaggagt cctcaccgcc acccaagaca cctccctcca agacggatgc
     1741 ctcatctaca acgtcaagat ccgtggagtc aacttcacct ccaacggacc agtcatgcaa
     1801 aagaagaccc tcggatggga gaccttcacc gagaccctct acccagccga cggaggacta
     1861 gagggacgta acgacatggc cctcaagctc gtcggaggct cccacctcat cgccaacgcc
     1921 aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagaccacc
     1981 taccgttcca agaagccagc caagaacctc aagatgccag gagtctacta cgttgactac
     2041 cgtctagagc gtatcaagga ggccaacaac gagacctacg tcgagcaaca cgaggtcgcc
     2101 gtcgcccgtt actgcgacct cccatccaag ctcggacaca agctcaacta aaagctttgg
     2161 aattcgctag ccggccatac aagtaatccg gatgatcgac gccaacgtcg ttgaattttc
     2221 aaattttaaa tactgaatat ttgttttttt tcctattatt tatttattct ctttgtgttt
     2281 tttttcttgc tttctaaaaa attaattcaa tccaaatcta aacatgaggg gcccggtacc
     2341 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     2401 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2461 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2521 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2581 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2641 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2701 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2761 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2821 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2881 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2941 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3001 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3061 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3121 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3181 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     3241 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3301 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3361 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3421 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3481 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3541 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3601 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3661 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3721 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3781 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3841 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3901 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3961 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4021 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4081 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4141 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4201 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4261 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4321 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4381 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4441 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4501 acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
