LOCUS       Exported                5019 bp ds-DNA     circular SYN 04-MAY-2017
DEFINITION  (unc-119 shortened rescue fragment; 
            unc-14pro::his-24::wormCherry::let-858 3'UTR; amp resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 1, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2073..2806
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
     misc_feature    691..696
                     /label=Post Promoter
     misc_feature    697..1341
                     /label=his-24 CDS
                     /label=his-24\CDS
     misc_feature    1348..2058
                     /label=mOrange
     misc_feature    2073..2806
                     /label=unc-54 3' UTR
                     /note="unc-54 3' UTR"
     misc_feature    2090..2115
                     /label=Z21 rev seq primer
                     /note="Z21 rev seq primer"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggacta gtggatcccc cgggctgcag cctaggatgt ctgattccgc tgttgttgcc
      721 gccgctgtcg agccaaaggt cccaaaggct aaggccgcca aggccgccaa gccagccgcc
      781 aaggccgcca agccaaccaa agttgccaag gccaaggctc cagtcgctca cccaccatac
      841 atcaacatga tcaaggaggc catcaagcag ctcaaggacc gcaaaggagc ttccaagcag
      901 gcaatcctca agttcatctc ccaaaactac aagctcggag acaatgtcat ccagatcaat
      961 gctcatctcc gtcaggctct caagcgtggt gtcaccagca aggcccttgt tcaagccgcc
     1021 ggatccggag ccaacggacg tttccgtgtg ccagagaagg ccgccgccgc caagaagcca
     1081 gcagcagcca agaagccagc agcagccaag aaaccagcag ccgccaagaa agccactgga
     1141 gagaagaagg caaagaagcc agccgctgcc aagccaaaga aggccgccac cggagataag
     1201 aaggtcaaga aggccaagag cccaaagaag gttgccaagc cagctgccaa gaaggtcgcc
     1261 aagtctccag ccaagaaggc cgctccaaag aagatcgcca agccagctgc caagaaggcc
     1321 gccaagccag ccgccaaggc cgcatgcatg gtgagcaagg gcgaggagaa taacatggcc
     1381 atcatcaagg agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag
     1441 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gctttcagac cgctaagctg
     1501 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcatttcacc
     1561 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt caagctgtcc
     1621 ttccccgagg gcttcaagtg ggagcgcgtg atgaactacg aggacggcgg cgtggtgacc
     1681 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     1741 accaacttcc cctccgacgg ccccgtgatg cagaagaaga ccatgggctg ggaggcctcc
     1801 tccgagcgga tgtaccccga ggacggtgcc ctgaagggca agatcaagat gaggctgaag
     1861 ctgaaggacg gcggccacta cacctccgag gtcaagacca cctacaaggc caagaagccc
     1921 gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcacctc ccacaacgag
     1981 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     2041 gacgagctgt acaagtaaaa gctttggaat tcatctcgcg cccgtgcctc tgacttctaa
     2101 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     2161 ttgttattat caaaaaaact tcttcttaat ttctttgttt tttagcttct tttaagtcac
     2221 ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa
     2281 aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac acaatgttct
     2341 gtgtacactt cttatgtttt ttttacttct gataaatttt ttttgaaaca tcatagaaaa
     2401 aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt
     2461 tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga
     2521 gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct
     2581 ttttgggggt ttcccctatt gtttgtcaag agtttcgagg acggcgtttt tcttgctaaa
     2641 atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct
     2701 cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg
     2761 ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttgggc ccggtaccca
     2821 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     2881 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2941 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     3001 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     3061 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3121 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3181 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3241 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3301 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3361 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3421 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3481 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3541 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3601 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3661 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     3721 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3781 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3841 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3901 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3961 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4021 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4081 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4141 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4201 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4261 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4321 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4381 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4441 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4501 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4561 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4621 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4681 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4741 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4801 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4861 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4921 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4981 aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
