LOCUS       Exported                4598 bp ds-DNA     circular SYN 04-MAY-2017
DEFINITION  (unc-119 shortened rescue fragment; 
            unc-14pro::his-24::wormCherry::let-858 3'UTR; amp resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 1, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    691..696
                     /label=Post Promoter
     misc_feature    697..1341
                     /label=his-24 CDS
                     /label=his-24\CDS
     misc_feature    1348..2208
                     /label=mCherry
     3'UTR           2223..2385
                     /label=let-858 3'UTR
                     /label=let-858\3'UTR
                     /note="/vntifkey=50"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggacta gtggatcccc cgggctgcag cctaggatgt ctgattccgc tgttgttgcc
      721 gccgctgtcg agccaaaggt cccaaaggct aaggccgcca aggccgccaa gccagccgcc
      781 aaggccgcca agccaaccaa agttgccaag gccaaggctc cagtcgctca cccaccatac
      841 atcaacatga tcaaggaggc catcaagcag ctcaaggacc gcaaaggagc ttccaagcag
      901 gcaatcctca agttcatctc ccaaaactac aagctcggag acaatgtcat ccagatcaat
      961 gctcatctcc gtcaggctct caagcgtggt gtcaccagca aggcccttgt tcaagccgcc
     1021 ggatccggag ccaacggacg tttccgtgtg ccagagaagg ccgccgccgc caagaagcca
     1081 gcagcagcca agaagccagc agcagccaag aaaccagcag ccgccaagaa agccactgga
     1141 gagaagaagg caaagaagcc agccgctgcc aagccaaaga aggccgccac cggagataag
     1201 aaggtcaaga aggccaagag cccaaagaag gttgccaagc cagctgccaa gaaggtcgcc
     1261 aagtctccag ccaagaaggc cgctccaaag aagatcgcca agccagctgc caagaaggcc
     1321 gccaagccag ccgccaaggc cgcatgcatg gtctcaaagg gtgaagaaga taacatggca
     1381 attattaaag agtttatgcg tttcaaggtg catatggagg gatctgtcaa tgggcatgag
     1441 tttgaaattg aaggtgaagg agaaggccga ccatatgagg gaacacaaac cgcaaaacta
     1501 aaggtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcaggtaact
     1561 aaaggcggac cattaccatt cgcctgggac atcctctctc cacagttcat gtatggaagt
     1621 aaagcttatg ttaaacatcc ggcagatata ccagattatt tgaaactttc attcccggag
     1681 ggttttaagt gggaacgcgt aatgaatttt gaagacggag gagttgttac agtgacgcaa
     1741 gactcaaggt aagtttaaac agttcggtac taactaacca tacatattta aattttcagc
     1801 ctccaagatg gagaatttat ttataaagtc aaacttcgag gaacgaattt cccctcggat
     1861 ggacctgtta tgcagaagaa gactatggga tgggaagctt caagtgaaag aatgtaccct
     1921 gaagacggtg ctcttaaggg agagattaaa caacgtctta aattgaaaga tggaggacat
     1981 tacgatgctg aggtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt
     2041 caggtgaaga caacttacaa agccaaaaaa ccagttcagc tgccaggagc gtacaatgtt
     2101 aatattaaac tggatatcac ctcccacaac gaggattaca ctatcgttga gcaatatgaa
     2161 agagctgaag ggcggcactc gacaggtggc atggatgaat tgtataagaa gctttggaat
     2221 tcgctagccg gccatacaag taatccggat gatcgacgcc aacgtcgttg aattttcaaa
     2281 ttttaaatac tgaatatttg ttttttttcc tattatttat ttattctctt tgtgtttttt
     2341 ttcttgcttt ctaaaaaatt aattcaatcc aaatctaaac atgaggggcc cggtacccag
     2401 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     2461 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2521 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2581 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2641 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2701 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2761 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2821 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2881 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2941 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3001 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3061 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3121 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3181 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3241 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     3301 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3361 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3421 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3481 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3541 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3601 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3661 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3721 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3781 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3841 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3901 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3961 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4021 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4081 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4141 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4201 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4261 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4321 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4381 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4441 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4501 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4561 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
