LOCUS       Exported                8356 bp ds-DNA     circular SYN 04-MAY-2017
DEFINITION  (unc-119 shortened rescue fragment; 
            unc-14pro::his-24::wormCherry::let-858 3'UTR; amp resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 1, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5410..6143
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
     misc_feature    691..4018
                     /label=Plim-4
     misc_feature    4019..4024
                     /label=Post Promoter
     misc_feature    4025..4669
                     /label=his-24 CDS
                     /label=his-24\CDS
     misc_feature    4676..5395
                     /label=Cerulean
     misc_feature    5410..6143
                     /label=unc-54 3' UTR
                     /note="unc-54 3' UTR"
     misc_feature    5427..5452
                     /label=Z21 rev seq primer
                     /note="Z21 rev seq primer"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggacta gtggatcccc cgggctgcag aagctttgat ttagaaattg tagtttccta
      721 agcctaaatg tacatattac ttttgggtta tgcctgagaa ttaccaatga ttgttttgtt
      781 caataagaat tatcatttac agagaaacaa gatccattgc ttttatcgat tcgagttgtg
      841 actttttagg ataaaatttc cttgaaaaac gttacagcga cagacatcat tttcgacgtt
      901 gaatctgaaa tcgtctgtaa ctcaagaaaa aatcgcttcc acgaaaataa atttatataa
      961 agttattttc ataaaattaa ctgggacttt tgggtgattt tgggagtttt cggcaaacca
     1021 tcaaatcgct aattgacaaa ataacaaaaa aggtatttaa acgattttta gataaaattc
     1081 ttaaatttta aaacagtgaa actaaaacgg gctggcattt atccttgtag cgactcaact
     1141 taatattaga acttgacttc acaatttaac agacacgacg tctgaggaac tttttcggtt
     1201 gtttgcattt ttgatagctc actgttttat gcccatatac gttttatttc ttatttatct
     1261 caaaccgtct tccctcaaac ttgaataaat cttcacaaga ctttttggga gtttctggcg
     1321 gaccatcaaa tcgctaattg aattcgcgca ttttcgtgtt ttgagtgcgt ccgcatcctt
     1381 gcgaggggaa atacgattta catcgatctc gagataattc gattcaagct gttgcacacg
     1441 tcctcccgac aaacactgga gaagagcacc caccagccac cgacggtcga cgacttttga
     1501 cttcaaattg gatgtcaaca actttctatt aagcatgttt tgaaatggga tacaaataaa
     1561 agtaaaagaa agagattcaa acatggatat tgaaaaaaag taattcagat atacactata
     1621 aagagggcag actattagta tggttttttt tcaaatgaag gagtgacacg gagtatctaa
     1681 aaggttaaca aagttaaagg ttaaaaataa catttgaaaa aagatttaat ctagaattat
     1741 acgaattttc aggtaatgct tttttgatgt gaaaatgcaa tttgaactct gaaaagttgg
     1801 taaatcaagg ttattttatt ttagatcaaa actgagcggc ttgttcgtcg gctggtagat
     1861 caaatgtgaa ctttttaatg tttcagcacg tactgatttg gaaattgtta cttcagttga
     1921 ataaaactag taaatcaaaa aaaaatctaa aatttaccat gtgttggcgg aattgattta
     1981 ttgtgatgtg tattggattt tttgtgtatt attgtttctt atgacacgat tcgtttggaa
     2041 tcgtattaaa taaataaaaa ataaaaaaaa aattgtccac acgttcaaga aatacgtaat
     2101 tttaacctac cagccgacag ccgacaaccg acgtctgaac actataaaaa tatctaaatc
     2161 gtggctggtt ttttttgaag ttttttttct aagaagtatt tcaagatcaa ccaatcaaaa
     2221 ttttataatt attctcaaat tgtcattttc tgatactgtg aaaagtaaac aattttttac
     2281 cagtggttta gatttgctgc atgttttttt tttcatttcc agtcagtttc tggagcaaaa
     2341 taacgtccta tatctcttca ggaagaggtt tttcagattt caattccaat cttcaaccct
     2401 cccacaatcc taacaactaa tagtattaat tttggtagaa taataagatg cttccaattt
     2461 gatgcacacg tgtcgagtgg aatgcgccaa atggtggtgt gtaggtgtgc ttcttccccg
     2521 tctgttgttc tttttgagag cccccagagc caattcacat taatccgata actcttgtta
     2581 tatatgtatg tgtatcgaat ccgaaaggat cactatttta acaatagtcc ttcggtatca
     2641 ctacctcatt catgttttcc tcagcggcat cctatgaaaa cggcaggcct ggcagattgt
     2701 gcgacatgaa ttgatgctca ttagcatgag acatccaagt cctcaagact ggtggcagta
     2761 acaagcaaac tagtcaggag cctccaattt tggtggctct attatctggc cgaacaattt
     2821 acccttattg aaacagatat tagtcacctt ttcctatttt ttccgaaaaa cttggaaacg
     2881 taatatggga tacgcagtag ccatggtttc attatttgag agaggggttg caaaatatct
     2941 ctacttactt attatcttct ttatgataat attttgcctc caaagccatt atcacacata
     3001 ttttattcgc aacggacacc taggatcatt tttgaaactt gtgtagtata accccacatt
     3061 ttgaatacat agttcgaatt tattgtatag aaaaatacaa atttctaagg ctgaaacgca
     3121 atatgtgtat cattttaatt aaattggatg aaattctaga gttctaacat aatttttaat
     3181 aaacaggatt ataatattca agtcccatat tgccttgcac ccaattgttt aattttgtca
     3241 gcttttatct catttttagt ttttttttgc attttcactg tattctagag gtggacagca
     3301 attctacaaa attacctatg ttccattttg ttccaacttt ttctattgaa aattgatatt
     3361 aaattaaggg aaattactta attagtttga aaatgacatt cattgaaacg ttcaagaaaa
     3421 tgctgaaaat tggttgattt tcaagtatcg aatagtcgaa atatttaatt tttcaggaca
     3481 gtttagagca cttttgaaaa cgtttcagct aaaataaata gattttttcg gaaaaaaaaa
     3541 ccatctcaaa atatgcattt ttgttcaatg gcctggatgc cagaccattt cttaaaatgt
     3601 ttaatgctct aataaaaact atcaaactaa tttttttttt gaaatttagc tgtttattta
     3661 ttcatacttc atatgatatt aattattctc gatattaaca ctttatttgc acttgataag
     3721 cattaaataa tttcctattg ttataacttt aatttgggaa ctttcagatt ccggagatct
     3781 gggctccttc ttttgccgga cactcctacg caagcagcca accacatccg ccttgccgcc
     3841 caatagtgag catcccagtg ggcggggcga gcttctagtg aggccttttc gacagaacaa
     3901 cagactagaa ggaagacttt tgcgtcggtg ctcgtcgact cgttagtggt ccttgttgtc
     3961 ttgttatcta cagctcggcc ggcagttcgt taagaaagca gctgtcacaa gttgcacccc
     4021 taggatgtct gattccgctg ttgttgccgc cgctgtcgag ccaaaggtcc caaaggctaa
     4081 ggccgccaag gccgccaagc cagccgccaa ggccgccaag ccaaccaaag ttgccaaggc
     4141 caaggctcca gtcgctcacc caccatacat caacatgatc aaggaggcca tcaagcagct
     4201 caaggaccgc aaaggagctt ccaagcaggc aatcctcaag ttcatctccc aaaactacaa
     4261 gctcggagac aatgtcatcc agatcaatgc tcatctccgt caggctctca agcgtggtgt
     4321 caccagcaag gcccttgttc aagccgccgg atccggagcc aacggacgtt tccgtgtgcc
     4381 agagaaggcc gccgccgcca agaagccagc agcagccaag aagccagcag cagccaagaa
     4441 accagcagcc gccaagaaag ccactggaga gaagaaggca aagaagccag ccgctgccaa
     4501 gccaaagaag gccgccaccg gagataagaa ggtcaagaag gccaagagcc caaagaaggt
     4561 tgccaagcca gctgccaaga aggtcgccaa gtctccagcc aagaaggccg ctccaaagaa
     4621 gatcgccaag ccagctgcca agaaggccgc caagccagcc gccaaggccg catgcatggt
     4681 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     4741 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     4801 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     4861 gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     4921 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     4981 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     5041 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     5101 ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     5161 caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     5221 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5281 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5341 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaaagct
     5401 ttggaattca tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc
     5461 tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaaacttct
     5521 tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga
     5581 ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat
     5641 taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt atgttttttt
     5701 tacttctgat aaattttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc
     5761 atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca
     5821 tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca
     5881 agtgaaagtt tatgaaatta attttcctgc ttttgctttt tgggggtttc ccctattgtt
     5941 tgtcaagagt ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga
     6001 tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg
     6061 ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc
     6121 caatatacca aacataactg tttgggcccg gtacccagct tttgttccct ttagtgaggg
     6181 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6241 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6301 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6361 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6421 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6481 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6541 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6601 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6661 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6721 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6781 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6841 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6901 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6961 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7021 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     7081 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7141 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7201 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7261 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7321 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7381 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7441 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7501 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7561 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7621 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7681 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7741 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7801 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7861 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7921 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7981 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8041 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8101 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8161 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8221 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8281 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8341 ccccgaaaag tgccac
//
