LOCUS       Exported                9402 bp ds-DNA     circular SYN 28-FEB-2019
DEFINITION  (unc-119 shortened rescue fragment; 
            unc-14pro::his-24::wormCherry::let-858 3'UTR; amp resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 1, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    691..5073
                     /label=rab-3 promoter
     misc_feature    5074..5079
                     /label=Post Promoter
     misc_feature    5080..5724
                     /label=his-24 CDS
                     /label=his-24\CDS
     misc_feature    5731..6441
                     /label=mOrange
     misc_feature    6456..7189
                     /label=unc-54 3' UTR
                     /note="unc-54 3' UTR"
     misc_feature    6473..6498
                     /label=Z21 rev seq primer
                     /note="Z21 rev seq primer"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggacta gtggatcccc cgggctgcag gcgagttttg actggctttc aaaaactgag
      721 tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag acatttttgc
      781 aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct ataatagttt
      841 ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt gaaagtatgc
      901 agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata taatctaggt
      961 taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc tttcaaatgg
     1021 tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc ggatttattc
     1081 caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt gaaaaaatga
     1141 gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg agggttccgt
     1201 gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag ccacactgtt
     1261 ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag atgctttcat
     1321 tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa ttatgcatga
     1381 gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg tgtggtttaa
     1441 tctgtgaaag ttttgaatct tctgtttgaa aaaaaaacat ttttaatttg tatatacgag
     1501 tgaattggtt aggccctggt tacgttttcc agcgaattca ttcaaatttc ttattttttc
     1561 tattttttca attaaataat ttagttctct atcacatgtg tatgatcaaa atttcattcc
     1621 taatcgtaag caaatttagg ctcatccgta gtataatttt gttatgaagg gcttcacagt
     1681 aaaagctcaa actccaacat ttagcaacta gcaaccaaca aaaaaccaaa gaaatgtcag
     1741 ttttgaaaag tcgttatttc tttgtcttct cacaattcac acatcatctt ctcgttttcc
     1801 tccgtttttt tgtcgtgctt ccttcttttc tttacacatt tttctaacct ttttttaagt
     1861 ctatcctctc ctaatccttt gtactctcta atcagttttg ctggttgtgc tgcacaattt
     1921 ttatcagatt tatgcgcgaa acaaaagcct ctcgcggaga gctccgccaa atccggtctt
     1981 cttttttcct tgcctatcgg atttcacaaa cattcagttt gtcacttgac gatgtgttgg
     2041 aggcattact actaggaagt gttattcata ttttttattc gtttcttttt ggtgactaaa
     2101 cgaaatattc cgaacgtttt cctgatttca ggcggcggaa acgaagaaga ccatgaataa
     2161 tcaacaggct gccattgcat ccgccagaag tcgagtgagt tctttgaaaa tctttattag
     2221 agttatttgt gacaatttgc cacttttttt ttaatgttta atgaatctgt aacgaaatta
     2281 ataccaaggg tgtccaacag aactatctga gcggttattt aatttttaga tagtatattt
     2341 cttccgatcg taacatacat attttcaagc atagtactgt gatttaagtt acagttttcc
     2401 aatcagtctt gattttaata aaaacttttc aactgcaaaa atgcctctga agtatttttc
     2461 tatattttta tagttgaata attttttgat tggcacaaag accgccgaga tatgagccgt
     2521 taaagtaaag ctgcatggat tcaatatggt ttgtatttga ttggagtcac ttacatggaa
     2581 atatttgtat ttaaatctca tattttaaaa caagacaaac accaaattaa atttttatta
     2641 atctgaacat ttttcaatcg atttggtaaa caaattatta gatgtttccc tcaaaaattg
     2701 taacaattcc taaattgtgg cacttataat aacatcgtcc tgaattctca aaaaaaaccg
     2761 aatgctaaga gagatttcgt ttaactggga cctgtaactg aattttcaaa tattggcagt
     2821 ttttgataaa ctgggaggga aatcaaaaac caaaactaca gtacccctcg tgttataata
     2881 aattgtcatt cactcctttt ttgttcccaa catttgctcc catctcctga cgagaaagaa
     2941 caattttatg agcacgggaa cccgtgggcg tccatccgtt cctcctgtgc aatttcagcc
     3001 cccttcctct acattctctg tgttcccgtt tttatcgttt tatgggtatc gggaacggac
     3061 agatcgcttc catttgcttc tattccgtcc tcacaagacg tcccactaac tgattttctt
     3121 ctgatttctt ttcactgaaa atgggaaaga aagaaaaaga cgatgaatcg acgacttctt
     3181 ctcaggagaa ggtgaagaag aaggataagg agaaagttca tctaaggctg aaagataaag
     3241 taaaaaaact tagaaaatct aagtcgattg cagtatcttc tccagctgat tatatgactt
     3301 gtcagtgtga accatgcaaa aaagtagctc tcgaggtaat aatttttaaa ctcaatacgt
     3361 agttaagttt gaactccttt taatttactt atcatctaat ttaccgtcac aatagtactt
     3421 ccattagcaa ttattttaaa attacatgca tagcttcaat gttcgttttt ttgcgttctc
     3481 atatcgtatg ttgtctgcct ctcgaatact gactgtccga ctagaatgtc ccggtaggat
     3541 tactgtacgg tcactgggaa taaactgttt ctgaaatcaa tctgaaattc cgtagatctt
     3601 cttggttcaa tttcttattc ctcaatcttt ggacaacgtc accttcagag tcgtcgttga
     3661 gtatcccatc attaactagt caatctttct ctctttttca tttcccccaa cttttctgca
     3721 tattttttga cgacgacgac ctcgacggca acgagaatac atttatctat tttttagtat
     3781 agcatatata cattttctac ttttctctaa cccatttcag acgtatagat tttggttttc
     3841 gaatgcaact ttgagatttt tcaaagatct tcagatggga gcagtggact gtctatttga
     3901 tattcttaaa atgtactcta taaaagatga aactgtaatg accaattccc tacataaata
     3961 tttttaacta attggtcgga tatttgggga tcagagaagt acttgttttt gcgatattaa
     4021 taatgatata tttttctagt aaaaaaatgt taagcgattt gtgaataatt aagttggctt
     4081 gaaaaagatt ttttgatagc tatgtatttt gttaatttaa atcagatttt aaaactttat
     4141 tattgactat ttatatatat tatatattat atattttcat ggtttaatca aaatctatta
     4201 aattatttct aaaatctcta aatatcgaat tctctaaaaa gcttcatcaa caaaagcatt
     4261 tcaattatct aacacaatga gaataattca agaactgaaa tagaaagaaa tctagaaaaa
     4321 aaagagagtg aggtgcccta ttaaatcggt gctctcctct ctaacagaag cgcctacacg
     4381 cacccaagag agctcttttt ctttcttttt tcggagcaca catacacgca tacactcctc
     4441 agaagtgtat agagaaaatg ggagaagaag cgaaagaaga cgaagaagcg gttgtgatgt
     4501 atgcatcggg cgtttctatc tctctccgtg agcaacgagc tagtcaaccc atcgatccat
     4561 ttttgtgagc acacacagag agagactcaa acacactcac agcttcacac tgagctccaa
     4621 gagctctgta gagagaagcc agagtatgct ttcctcgatt ttcctaacgt cttcttcttc
     4681 tgcttttttc tttttcccaa cgagttgtag tccactaact tcaatttttc ctccagagaa
     4741 agagttttag agagacagct gataaataca ctagagaaat aaaaagaatg ataaaaattt
     4801 gctattttaa tcagttccct ctcgtttctc taagttttgt tgttgggttg ggaaattaaa
     4861 attggaaaat ggaaataacg tttttttctg tctaatcttg aattctccag acatttctta
     4921 tttccttttt tgttgcaagt ttttcttcct tctctaattt ttaatcaaag atatctcgct
     4981 atgaactttg aatttttgat attttgattc tataaataca taaagtaatc taaatgctct
     5041 tttaaaataa atctacagta gccctatttt cagcctagga tgtctgattc cgctgttgtt
     5101 gccgccgctg tcgagccaaa ggtcccaaag gctaaggccg ccaaggccgc caagccagcc
     5161 gccaaggccg ccaagccaac caaagttgcc aaggccaagg ctccagtcgc tcacccacca
     5221 tacatcaaca tgatcaagga ggccatcaag cagctcaagg accgcaaagg agcttccaag
     5281 caggcaatcc tcaagttcat ctcccaaaac tacaagctcg gagacaatgt catccagatc
     5341 aatgctcatc tccgtcaggc tctcaagcgt ggtgtcacca gcaaggccct tgttcaagcc
     5401 gccggatccg gagccaacgg acgtttccgt gtgccagaga aggccgccgc cgccaagaag
     5461 ccagcagcag ccaagaagcc agcagcagcc aagaaaccag cagccgccaa gaaagccact
     5521 ggagagaaga aggcaaagaa gccagccgct gccaagccaa agaaggccgc caccggagat
     5581 aagaaggtca agaaggccaa gagcccaaag aaggttgcca agccagctgc caagaaggtc
     5641 gccaagtctc cagccaagaa ggccgctcca aagaagatcg ccaagccagc tgccaagaag
     5701 gccgccaagc cagccgccaa ggccgcatgc atggtgagca agggcgagga gaataacatg
     5761 gccatcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac
     5821 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggctttca gaccgctaag
     5881 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcatttc
     5941 acctacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttcaagctg
     6001 tccttccccg agggcttcaa gtgggagcgc gtgatgaact acgaggacgg cggcgtggtg
     6061 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     6121 ggcaccaact tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc
     6181 tcctccgagc ggatgtaccc cgaggacggt gccctgaagg gcaagatcaa gatgaggctg
     6241 aagctgaagg acggcggcca ctacacctcc gaggtcaaga ccacctacaa ggccaagaag
     6301 cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt tggacatcac ctcccacaac
     6361 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     6421 atggacgagc tgtacaagta aaagctttgg aattcatctc gcgcccgtgc ctctgacttc
     6481 taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
     6541 tttttgttat tatcaaaaaa acttcttctt aatttctttg ttttttagct tcttttaagt
     6601 cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg
     6661 aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt
     6721 tctgtgtaca cttcttatgt tttttttact tctgataaat tttttttgaa acatcataga
     6781 aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt
     6841 atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt
     6901 ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt
     6961 gctttttggg ggtttcccct attgtttgtc aagagtttcg aggacggcgt ttttcttgct
     7021 aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag
     7081 gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt
     7141 ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttg ggcccggtac
     7201 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     7261 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7321 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7381 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7441 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7501 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7561 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7981 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8281 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8341 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8401 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8461 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8521 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8581 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8641 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8701 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8761 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8821 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8881 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8941 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9001 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9061 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9121 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9181 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9241 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9301 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9361 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
