LOCUS       Exported                9552 bp ds-DNA     circular SYN 28-FEB-2019
DEFINITION  (unc-119 shortened rescue fragment; 
            unc-14pro::his-24::wormCherry::let-858 3'UTR; amp resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 1, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9552
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    691..5073
                     /label=rab-3 promoter
     misc_feature    5074..5079
                     /label=Post Promoter
     misc_feature    5080..5724
                     /label=his-24 CDS
                     /label=his-24\CDS
     misc_feature    5731..6591
                     /label=mCherry
     misc_feature    6606..7339
                     /label=unc-54 3' UTR
                     /note="unc-54 3' UTR"
     misc_feature    6623..6648
                     /label=Z21 rev seq primer
                     /note="Z21 rev seq primer"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggacta gtggatcccc cgggctgcag gcgagttttg actggctttc aaaaactgag
      721 tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag acatttttgc
      781 aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct ataatagttt
      841 ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt gaaagtatgc
      901 agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata taatctaggt
      961 taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc tttcaaatgg
     1021 tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc ggatttattc
     1081 caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt gaaaaaatga
     1141 gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg agggttccgt
     1201 gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag ccacactgtt
     1261 ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag atgctttcat
     1321 tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa ttatgcatga
     1381 gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg tgtggtttaa
     1441 tctgtgaaag ttttgaatct tctgtttgaa aaaaaaacat ttttaatttg tatatacgag
     1501 tgaattggtt aggccctggt tacgttttcc agcgaattca ttcaaatttc ttattttttc
     1561 tattttttca attaaataat ttagttctct atcacatgtg tatgatcaaa atttcattcc
     1621 taatcgtaag caaatttagg ctcatccgta gtataatttt gttatgaagg gcttcacagt
     1681 aaaagctcaa actccaacat ttagcaacta gcaaccaaca aaaaaccaaa gaaatgtcag
     1741 ttttgaaaag tcgttatttc tttgtcttct cacaattcac acatcatctt ctcgttttcc
     1801 tccgtttttt tgtcgtgctt ccttcttttc tttacacatt tttctaacct ttttttaagt
     1861 ctatcctctc ctaatccttt gtactctcta atcagttttg ctggttgtgc tgcacaattt
     1921 ttatcagatt tatgcgcgaa acaaaagcct ctcgcggaga gctccgccaa atccggtctt
     1981 cttttttcct tgcctatcgg atttcacaaa cattcagttt gtcacttgac gatgtgttgg
     2041 aggcattact actaggaagt gttattcata ttttttattc gtttcttttt ggtgactaaa
     2101 cgaaatattc cgaacgtttt cctgatttca ggcggcggaa acgaagaaga ccatgaataa
     2161 tcaacaggct gccattgcat ccgccagaag tcgagtgagt tctttgaaaa tctttattag
     2221 agttatttgt gacaatttgc cacttttttt ttaatgttta atgaatctgt aacgaaatta
     2281 ataccaaggg tgtccaacag aactatctga gcggttattt aatttttaga tagtatattt
     2341 cttccgatcg taacatacat attttcaagc atagtactgt gatttaagtt acagttttcc
     2401 aatcagtctt gattttaata aaaacttttc aactgcaaaa atgcctctga agtatttttc
     2461 tatattttta tagttgaata attttttgat tggcacaaag accgccgaga tatgagccgt
     2521 taaagtaaag ctgcatggat tcaatatggt ttgtatttga ttggagtcac ttacatggaa
     2581 atatttgtat ttaaatctca tattttaaaa caagacaaac accaaattaa atttttatta
     2641 atctgaacat ttttcaatcg atttggtaaa caaattatta gatgtttccc tcaaaaattg
     2701 taacaattcc taaattgtgg cacttataat aacatcgtcc tgaattctca aaaaaaaccg
     2761 aatgctaaga gagatttcgt ttaactggga cctgtaactg aattttcaaa tattggcagt
     2821 ttttgataaa ctgggaggga aatcaaaaac caaaactaca gtacccctcg tgttataata
     2881 aattgtcatt cactcctttt ttgttcccaa catttgctcc catctcctga cgagaaagaa
     2941 caattttatg agcacgggaa cccgtgggcg tccatccgtt cctcctgtgc aatttcagcc
     3001 cccttcctct acattctctg tgttcccgtt tttatcgttt tatgggtatc gggaacggac
     3061 agatcgcttc catttgcttc tattccgtcc tcacaagacg tcccactaac tgattttctt
     3121 ctgatttctt ttcactgaaa atgggaaaga aagaaaaaga cgatgaatcg acgacttctt
     3181 ctcaggagaa ggtgaagaag aaggataagg agaaagttca tctaaggctg aaagataaag
     3241 taaaaaaact tagaaaatct aagtcgattg cagtatcttc tccagctgat tatatgactt
     3301 gtcagtgtga accatgcaaa aaagtagctc tcgaggtaat aatttttaaa ctcaatacgt
     3361 agttaagttt gaactccttt taatttactt atcatctaat ttaccgtcac aatagtactt
     3421 ccattagcaa ttattttaaa attacatgca tagcttcaat gttcgttttt ttgcgttctc
     3481 atatcgtatg ttgtctgcct ctcgaatact gactgtccga ctagaatgtc ccggtaggat
     3541 tactgtacgg tcactgggaa taaactgttt ctgaaatcaa tctgaaattc cgtagatctt
     3601 cttggttcaa tttcttattc ctcaatcttt ggacaacgtc accttcagag tcgtcgttga
     3661 gtatcccatc attaactagt caatctttct ctctttttca tttcccccaa cttttctgca
     3721 tattttttga cgacgacgac ctcgacggca acgagaatac atttatctat tttttagtat
     3781 agcatatata cattttctac ttttctctaa cccatttcag acgtatagat tttggttttc
     3841 gaatgcaact ttgagatttt tcaaagatct tcagatggga gcagtggact gtctatttga
     3901 tattcttaaa atgtactcta taaaagatga aactgtaatg accaattccc tacataaata
     3961 tttttaacta attggtcgga tatttgggga tcagagaagt acttgttttt gcgatattaa
     4021 taatgatata tttttctagt aaaaaaatgt taagcgattt gtgaataatt aagttggctt
     4081 gaaaaagatt ttttgatagc tatgtatttt gttaatttaa atcagatttt aaaactttat
     4141 tattgactat ttatatatat tatatattat atattttcat ggtttaatca aaatctatta
     4201 aattatttct aaaatctcta aatatcgaat tctctaaaaa gcttcatcaa caaaagcatt
     4261 tcaattatct aacacaatga gaataattca agaactgaaa tagaaagaaa tctagaaaaa
     4321 aaagagagtg aggtgcccta ttaaatcggt gctctcctct ctaacagaag cgcctacacg
     4381 cacccaagag agctcttttt ctttcttttt tcggagcaca catacacgca tacactcctc
     4441 agaagtgtat agagaaaatg ggagaagaag cgaaagaaga cgaagaagcg gttgtgatgt
     4501 atgcatcggg cgtttctatc tctctccgtg agcaacgagc tagtcaaccc atcgatccat
     4561 ttttgtgagc acacacagag agagactcaa acacactcac agcttcacac tgagctccaa
     4621 gagctctgta gagagaagcc agagtatgct ttcctcgatt ttcctaacgt cttcttcttc
     4681 tgcttttttc tttttcccaa cgagttgtag tccactaact tcaatttttc ctccagagaa
     4741 agagttttag agagacagct gataaataca ctagagaaat aaaaagaatg ataaaaattt
     4801 gctattttaa tcagttccct ctcgtttctc taagttttgt tgttgggttg ggaaattaaa
     4861 attggaaaat ggaaataacg tttttttctg tctaatcttg aattctccag acatttctta
     4921 tttccttttt tgttgcaagt ttttcttcct tctctaattt ttaatcaaag atatctcgct
     4981 atgaactttg aatttttgat attttgattc tataaataca taaagtaatc taaatgctct
     5041 tttaaaataa atctacagta gccctatttt cagcctagga tgtctgattc cgctgttgtt
     5101 gccgccgctg tcgagccaaa ggtcccaaag gctaaggccg ccaaggccgc caagccagcc
     5161 gccaaggccg ccaagccaac caaagttgcc aaggccaagg ctccagtcgc tcacccacca
     5221 tacatcaaca tgatcaagga ggccatcaag cagctcaagg accgcaaagg agcttccaag
     5281 caggcaatcc tcaagttcat ctcccaaaac tacaagctcg gagacaatgt catccagatc
     5341 aatgctcatc tccgtcaggc tctcaagcgt ggtgtcacca gcaaggccct tgttcaagcc
     5401 gccggatccg gagccaacgg acgtttccgt gtgccagaga aggccgccgc cgccaagaag
     5461 ccagcagcag ccaagaagcc agcagcagcc aagaaaccag cagccgccaa gaaagccact
     5521 ggagagaaga aggcaaagaa gccagccgct gccaagccaa agaaggccgc caccggagat
     5581 aagaaggtca agaaggccaa gagcccaaag aaggttgcca agccagctgc caagaaggtc
     5641 gccaagtctc cagccaagaa ggccgctcca aagaagatcg ccaagccagc tgccaagaag
     5701 gccgccaagc cagccgccaa ggccgcatgc atggtctcaa agggtgaaga agataacatg
     5761 gcaattatta aagagtttat gcgtttcaag gtgcatatgg agggatctgt caatgggcat
     5821 gagtttgaaa ttgaaggtga aggagaaggc cgaccatatg agggaacaca aaccgcaaaa
     5881 ctaaaggtaa gtttaaacat atatatacta actaaccctg attatttaaa ttttcaggta
     5941 actaaaggcg gaccattacc attcgcctgg gacatcctct ctccacagtt catgtatgga
     6001 agtaaagctt atgttaaaca tccggcagat ataccagatt atttgaaact ttcattcccg
     6061 gagggtttta agtgggaacg cgtaatgaat tttgaagacg gaggagttgt tacagtgacg
     6121 caagactcaa ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     6181 agcctccaag atggagaatt tatttataaa gtcaaacttc gaggaacgaa tttcccctcg
     6241 gatggacctg ttatgcagaa gaagactatg ggatgggaag cttcaagtga aagaatgtac
     6301 cctgaagacg gtgctcttaa gggagagatt aaacaacgtc ttaaattgaa agatggagga
     6361 cattacgatg ctgaggtaag tttaaacatg attttactaa ctaactaatc tgatttaaat
     6421 tttcaggtga agacaactta caaagccaaa aaaccagttc agctgccagg agcgtacaat
     6481 gttaatatta aactggatat cacctcccac aacgaggatt acactatcgt tgagcaatat
     6541 gaaagagctg aagggcggca ctcgacaggt ggcatggatg aattgtataa gaagctttgg
     6601 aattcatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca
     6661 tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa acttcttctt
     6721 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca
     6781 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat
     6841 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttttact
     6901 tctgataaat tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     6961 gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac
     7021 gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg
     7081 aaagtttatg aaattaattt tcctgctttt gctttttggg ggtttcccct attgtttgtc
     7141 aagagtttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca
     7201 agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa
     7261 tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat
     7321 ataccaaaca taactgtttg ggcccggtac ccagcttttg ttccctttag tgagggttaa
     7381 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7441 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7501 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7561 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7621 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7681 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7741 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7801 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7861 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7921 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7981 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8041 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8101 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8161 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8221 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8281 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8341 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8401 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8461 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8521 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8581 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8641 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8701 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8761 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8821 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8881 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8941 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9001 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9061 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9121 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9181 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9241 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9301 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9361 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9421 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9481 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9541 gaaaagtgcc ac
//
