LOCUS       Exported                9426 bp ds-DNA     circular SYN 28-FEB-2019
DEFINITION  (unc-119 shortened rescue fragment; 
            unc-14pro::his-24::wormCherry::let-858 3'UTR; amp resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 1, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    691..5073
                     /label=rab-3 promoter
     misc_feature    5074..5079
                     /label=Post Promoter
     misc_feature    5080..5724
                     /label=his-24 CDS
                     /label=his-24\CDS
     misc_feature    5731..6465
                     /label=mNeptune
     misc_feature    6480..7213
                     /label=unc-54 3' UTR
                     /note="unc-54 3' UTR"
     misc_feature    6497..6522
                     /label=Z21 rev seq primer
                     /note="Z21 rev seq primer"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggacta gtggatcccc cgggctgcag gcgagttttg actggctttc aaaaactgag
      721 tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag acatttttgc
      781 aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct ataatagttt
      841 ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt gaaagtatgc
      901 agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata taatctaggt
      961 taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc tttcaaatgg
     1021 tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc ggatttattc
     1081 caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt gaaaaaatga
     1141 gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg agggttccgt
     1201 gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag ccacactgtt
     1261 ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag atgctttcat
     1321 tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa ttatgcatga
     1381 gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg tgtggtttaa
     1441 tctgtgaaag ttttgaatct tctgtttgaa aaaaaaacat ttttaatttg tatatacgag
     1501 tgaattggtt aggccctggt tacgttttcc agcgaattca ttcaaatttc ttattttttc
     1561 tattttttca attaaataat ttagttctct atcacatgtg tatgatcaaa atttcattcc
     1621 taatcgtaag caaatttagg ctcatccgta gtataatttt gttatgaagg gcttcacagt
     1681 aaaagctcaa actccaacat ttagcaacta gcaaccaaca aaaaaccaaa gaaatgtcag
     1741 ttttgaaaag tcgttatttc tttgtcttct cacaattcac acatcatctt ctcgttttcc
     1801 tccgtttttt tgtcgtgctt ccttcttttc tttacacatt tttctaacct ttttttaagt
     1861 ctatcctctc ctaatccttt gtactctcta atcagttttg ctggttgtgc tgcacaattt
     1921 ttatcagatt tatgcgcgaa acaaaagcct ctcgcggaga gctccgccaa atccggtctt
     1981 cttttttcct tgcctatcgg atttcacaaa cattcagttt gtcacttgac gatgtgttgg
     2041 aggcattact actaggaagt gttattcata ttttttattc gtttcttttt ggtgactaaa
     2101 cgaaatattc cgaacgtttt cctgatttca ggcggcggaa acgaagaaga ccatgaataa
     2161 tcaacaggct gccattgcat ccgccagaag tcgagtgagt tctttgaaaa tctttattag
     2221 agttatttgt gacaatttgc cacttttttt ttaatgttta atgaatctgt aacgaaatta
     2281 ataccaaggg tgtccaacag aactatctga gcggttattt aatttttaga tagtatattt
     2341 cttccgatcg taacatacat attttcaagc atagtactgt gatttaagtt acagttttcc
     2401 aatcagtctt gattttaata aaaacttttc aactgcaaaa atgcctctga agtatttttc
     2461 tatattttta tagttgaata attttttgat tggcacaaag accgccgaga tatgagccgt
     2521 taaagtaaag ctgcatggat tcaatatggt ttgtatttga ttggagtcac ttacatggaa
     2581 atatttgtat ttaaatctca tattttaaaa caagacaaac accaaattaa atttttatta
     2641 atctgaacat ttttcaatcg atttggtaaa caaattatta gatgtttccc tcaaaaattg
     2701 taacaattcc taaattgtgg cacttataat aacatcgtcc tgaattctca aaaaaaaccg
     2761 aatgctaaga gagatttcgt ttaactggga cctgtaactg aattttcaaa tattggcagt
     2821 ttttgataaa ctgggaggga aatcaaaaac caaaactaca gtacccctcg tgttataata
     2881 aattgtcatt cactcctttt ttgttcccaa catttgctcc catctcctga cgagaaagaa
     2941 caattttatg agcacgggaa cccgtgggcg tccatccgtt cctcctgtgc aatttcagcc
     3001 cccttcctct acattctctg tgttcccgtt tttatcgttt tatgggtatc gggaacggac
     3061 agatcgcttc catttgcttc tattccgtcc tcacaagacg tcccactaac tgattttctt
     3121 ctgatttctt ttcactgaaa atgggaaaga aagaaaaaga cgatgaatcg acgacttctt
     3181 ctcaggagaa ggtgaagaag aaggataagg agaaagttca tctaaggctg aaagataaag
     3241 taaaaaaact tagaaaatct aagtcgattg cagtatcttc tccagctgat tatatgactt
     3301 gtcagtgtga accatgcaaa aaagtagctc tcgaggtaat aatttttaaa ctcaatacgt
     3361 agttaagttt gaactccttt taatttactt atcatctaat ttaccgtcac aatagtactt
     3421 ccattagcaa ttattttaaa attacatgca tagcttcaat gttcgttttt ttgcgttctc
     3481 atatcgtatg ttgtctgcct ctcgaatact gactgtccga ctagaatgtc ccggtaggat
     3541 tactgtacgg tcactgggaa taaactgttt ctgaaatcaa tctgaaattc cgtagatctt
     3601 cttggttcaa tttcttattc ctcaatcttt ggacaacgtc accttcagag tcgtcgttga
     3661 gtatcccatc attaactagt caatctttct ctctttttca tttcccccaa cttttctgca
     3721 tattttttga cgacgacgac ctcgacggca acgagaatac atttatctat tttttagtat
     3781 agcatatata cattttctac ttttctctaa cccatttcag acgtatagat tttggttttc
     3841 gaatgcaact ttgagatttt tcaaagatct tcagatggga gcagtggact gtctatttga
     3901 tattcttaaa atgtactcta taaaagatga aactgtaatg accaattccc tacataaata
     3961 tttttaacta attggtcgga tatttgggga tcagagaagt acttgttttt gcgatattaa
     4021 taatgatata tttttctagt aaaaaaatgt taagcgattt gtgaataatt aagttggctt
     4081 gaaaaagatt ttttgatagc tatgtatttt gttaatttaa atcagatttt aaaactttat
     4141 tattgactat ttatatatat tatatattat atattttcat ggtttaatca aaatctatta
     4201 aattatttct aaaatctcta aatatcgaat tctctaaaaa gcttcatcaa caaaagcatt
     4261 tcaattatct aacacaatga gaataattca agaactgaaa tagaaagaaa tctagaaaaa
     4321 aaagagagtg aggtgcccta ttaaatcggt gctctcctct ctaacagaag cgcctacacg
     4381 cacccaagag agctcttttt ctttcttttt tcggagcaca catacacgca tacactcctc
     4441 agaagtgtat agagaaaatg ggagaagaag cgaaagaaga cgaagaagcg gttgtgatgt
     4501 atgcatcggg cgtttctatc tctctccgtg agcaacgagc tagtcaaccc atcgatccat
     4561 ttttgtgagc acacacagag agagactcaa acacactcac agcttcacac tgagctccaa
     4621 gagctctgta gagagaagcc agagtatgct ttcctcgatt ttcctaacgt cttcttcttc
     4681 tgcttttttc tttttcccaa cgagttgtag tccactaact tcaatttttc ctccagagaa
     4741 agagttttag agagacagct gataaataca ctagagaaat aaaaagaatg ataaaaattt
     4801 gctattttaa tcagttccct ctcgtttctc taagttttgt tgttgggttg ggaaattaaa
     4861 attggaaaat ggaaataacg tttttttctg tctaatcttg aattctccag acatttctta
     4921 tttccttttt tgttgcaagt ttttcttcct tctctaattt ttaatcaaag atatctcgct
     4981 atgaactttg aatttttgat attttgattc tataaataca taaagtaatc taaatgctct
     5041 tttaaaataa atctacagta gccctatttt cagcctagga tgtctgattc cgctgttgtt
     5101 gccgccgctg tcgagccaaa ggtcccaaag gctaaggccg ccaaggccgc caagccagcc
     5161 gccaaggccg ccaagccaac caaagttgcc aaggccaagg ctccagtcgc tcacccacca
     5221 tacatcaaca tgatcaagga ggccatcaag cagctcaagg accgcaaagg agcttccaag
     5281 caggcaatcc tcaagttcat ctcccaaaac tacaagctcg gagacaatgt catccagatc
     5341 aatgctcatc tccgtcaggc tctcaagcgt ggtgtcacca gcaaggccct tgttcaagcc
     5401 gccggatccg gagccaacgg acgtttccgt gtgccagaga aggccgccgc cgccaagaag
     5461 ccagcagcag ccaagaagcc agcagcagcc aagaaaccag cagccgccaa gaaagccact
     5521 ggagagaaga aggcaaagaa gccagccgct gccaagccaa agaaggccgc caccggagat
     5581 aagaaggtca agaaggccaa gagcccaaag aaggttgcca agccagctgc caagaaggtc
     5641 gccaagtctc cagccaagaa ggccgctcca aagaagatcg ccaagccagc tgccaagaag
     5701 gccgccaagc cagccgccaa ggccgcatgc atggtgagca agggcgagga gctgatcaag
     5761 gagaacatgc acaccaagct gtacatggaa ggcaccgtga acaaccacca cttcaagtgc
     5821 acccacgaag gggagggcaa gccctacgag ggcacccaga ccaacaggat taaggtggtg
     5881 gagggaggcc ccctgccgtt cgcattcgac atcctggcca cctgctttat gtacgggagc
     5941 aagaccttca tcaaccacac ccagggcatc cccgatttct ttaagcagtc cttccctgag
     6001 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgcttac cgttacccag
     6061 gacaccagcc tccaggacgg ctgcttgatc tacaacgtca agctcagagg ggtgaacttc
     6121 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccag taccgagacc
     6181 ctgtaccccg ctgacggcgg cctggaaggc agatgcgaca tggccctgaa gctcgtgggc
     6241 gggggccacc tgcactgcaa cctgaagacc acatacagat ccaagaaacc cgctaagaac
     6301 ctcaagatgc ccggcgtcta ctttgtggac cgcagactgg aaagaatcaa ggaggccgac
     6361 aatgagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc
     6421 aaactggggc acaaacttaa tggcatggac gagctgtaca agtaaaagct ttggaattca
     6481 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct
     6541 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaaacttct tcttaatttc
     6601 tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata
     6661 gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat
     6721 tctatcccaa aatctacaca atgttctgtg tacacttctt atgttttttt tacttctgat
     6781 aaattttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg
     6841 tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa
     6901 tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt
     6961 tatgaaatta attttcctgc ttttgctttt tgggggtttc ccctattgtt tgtcaagagt
     7021 ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag
     7081 atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa
     7141 agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca
     7201 aacataactg tttgggcccg gtacccagct tttgttccct ttagtgaggg ttaattgcgc
     7261 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7321 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7381 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7441 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7501 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7561 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7621 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7681 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7741 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7801 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7861 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7921 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7981 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8041 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8101 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8161 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8221 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8281 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8341 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8401 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8461 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8521 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8581 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8641 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8701 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8761 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8821 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8881 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8941 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9001 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9061 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9121 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9181 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9241 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9301 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9361 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9421 tgccac
//
