LOCUS       Exported               10044 bp ds-DNA     circular SYN 01-AUG-2017
DEFINITION  (unc-119 shortened rescue fragment; 
            unc-14pro::his-24::wormCherry::let-858 3'UTR; amp resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 1, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10044
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          7098..7831
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
     misc_feature    691..5073
                     /label=rab-3 promoter
     misc_feature    5074..5079
                     /label=Post Promoter
     misc_feature    5080..5724
                     /label=his-24 CDS
                     /label=his-24\CDS
     misc_feature    5731..7083
                     /label=GCaMP6s
     misc_feature    5962..5964
                     /label=K78H
     misc_feature    6871..6873
                     /label=T381R
     misc_feature    6877..6879
                     /label=S383T
     misc_feature    6904..6906
                     /label=R392G
     misc_feature    7098..7831
                     /label=unc-54 3' UTR
                     /note="unc-54 3' UTR"
     misc_feature    7115..7140
                     /label=Z21 rev seq primer
                     /note="Z21 rev seq primer"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggacta gtggatcccc cgggctgcag gcgagttttg actggctttc aaaaactgag
      721 tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag acatttttgc
      781 aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct ataatagttt
      841 ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt gaaagtatgc
      901 agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata taatctaggt
      961 taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc tttcaaatgg
     1021 tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc ggatttattc
     1081 caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt gaaaaaatga
     1141 gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg agggttccgt
     1201 gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag ccacactgtt
     1261 ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag atgctttcat
     1321 tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa ttatgcatga
     1381 gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg tgtggtttaa
     1441 tctgtgaaag ttttgaatct tctgtttgaa aaaaaaacat ttttaatttg tatatacgag
     1501 tgaattggtt aggccctggt tacgttttcc agcgaattca ttcaaatttc ttattttttc
     1561 tattttttca attaaataat ttagttctct atcacatgtg tatgatcaaa atttcattcc
     1621 taatcgtaag caaatttagg ctcatccgta gtataatttt gttatgaagg gcttcacagt
     1681 aaaagctcaa actccaacat ttagcaacta gcaaccaaca aaaaaccaaa gaaatgtcag
     1741 ttttgaaaag tcgttatttc tttgtcttct cacaattcac acatcatctt ctcgttttcc
     1801 tccgtttttt tgtcgtgctt ccttcttttc tttacacatt tttctaacct ttttttaagt
     1861 ctatcctctc ctaatccttt gtactctcta atcagttttg ctggttgtgc tgcacaattt
     1921 ttatcagatt tatgcgcgaa acaaaagcct ctcgcggaga gctccgccaa atccggtctt
     1981 cttttttcct tgcctatcgg atttcacaaa cattcagttt gtcacttgac gatgtgttgg
     2041 aggcattact actaggaagt gttattcata ttttttattc gtttcttttt ggtgactaaa
     2101 cgaaatattc cgaacgtttt cctgatttca ggcggcggaa acgaagaaga ccatgaataa
     2161 tcaacaggct gccattgcat ccgccagaag tcgagtgagt tctttgaaaa tctttattag
     2221 agttatttgt gacaatttgc cacttttttt ttaatgttta atgaatctgt aacgaaatta
     2281 ataccaaggg tgtccaacag aactatctga gcggttattt aatttttaga tagtatattt
     2341 cttccgatcg taacatacat attttcaagc atagtactgt gatttaagtt acagttttcc
     2401 aatcagtctt gattttaata aaaacttttc aactgcaaaa atgcctctga agtatttttc
     2461 tatattttta tagttgaata attttttgat tggcacaaag accgccgaga tatgagccgt
     2521 taaagtaaag ctgcatggat tcaatatggt ttgtatttga ttggagtcac ttacatggaa
     2581 atatttgtat ttaaatctca tattttaaaa caagacaaac accaaattaa atttttatta
     2641 atctgaacat ttttcaatcg atttggtaaa caaattatta gatgtttccc tcaaaaattg
     2701 taacaattcc taaattgtgg cacttataat aacatcgtcc tgaattctca aaaaaaaccg
     2761 aatgctaaga gagatttcgt ttaactggga cctgtaactg aattttcaaa tattggcagt
     2821 ttttgataaa ctgggaggga aatcaaaaac caaaactaca gtacccctcg tgttataata
     2881 aattgtcatt cactcctttt ttgttcccaa catttgctcc catctcctga cgagaaagaa
     2941 caattttatg agcacgggaa cccgtgggcg tccatccgtt cctcctgtgc aatttcagcc
     3001 cccttcctct acattctctg tgttcccgtt tttatcgttt tatgggtatc gggaacggac
     3061 agatcgcttc catttgcttc tattccgtcc tcacaagacg tcccactaac tgattttctt
     3121 ctgatttctt ttcactgaaa atgggaaaga aagaaaaaga cgatgaatcg acgacttctt
     3181 ctcaggagaa ggtgaagaag aaggataagg agaaagttca tctaaggctg aaagataaag
     3241 taaaaaaact tagaaaatct aagtcgattg cagtatcttc tccagctgat tatatgactt
     3301 gtcagtgtga accatgcaaa aaagtagctc tcgaggtaat aatttttaaa ctcaatacgt
     3361 agttaagttt gaactccttt taatttactt atcatctaat ttaccgtcac aatagtactt
     3421 ccattagcaa ttattttaaa attacatgca tagcttcaat gttcgttttt ttgcgttctc
     3481 atatcgtatg ttgtctgcct ctcgaatact gactgtccga ctagaatgtc ccggtaggat
     3541 tactgtacgg tcactgggaa taaactgttt ctgaaatcaa tctgaaattc cgtagatctt
     3601 cttggttcaa tttcttattc ctcaatcttt ggacaacgtc accttcagag tcgtcgttga
     3661 gtatcccatc attaactagt caatctttct ctctttttca tttcccccaa cttttctgca
     3721 tattttttga cgacgacgac ctcgacggca acgagaatac atttatctat tttttagtat
     3781 agcatatata cattttctac ttttctctaa cccatttcag acgtatagat tttggttttc
     3841 gaatgcaact ttgagatttt tcaaagatct tcagatggga gcagtggact gtctatttga
     3901 tattcttaaa atgtactcta taaaagatga aactgtaatg accaattccc tacataaata
     3961 tttttaacta attggtcgga tatttgggga tcagagaagt acttgttttt gcgatattaa
     4021 taatgatata tttttctagt aaaaaaatgt taagcgattt gtgaataatt aagttggctt
     4081 gaaaaagatt ttttgatagc tatgtatttt gttaatttaa atcagatttt aaaactttat
     4141 tattgactat ttatatatat tatatattat atattttcat ggtttaatca aaatctatta
     4201 aattatttct aaaatctcta aatatcgaat tctctaaaaa gcttcatcaa caaaagcatt
     4261 tcaattatct aacacaatga gaataattca agaactgaaa tagaaagaaa tctagaaaaa
     4321 aaagagagtg aggtgcccta ttaaatcggt gctctcctct ctaacagaag cgcctacacg
     4381 cacccaagag agctcttttt ctttcttttt tcggagcaca catacacgca tacactcctc
     4441 agaagtgtat agagaaaatg ggagaagaag cgaaagaaga cgaagaagcg gttgtgatgt
     4501 atgcatcggg cgtttctatc tctctccgtg agcaacgagc tagtcaaccc atcgatccat
     4561 ttttgtgagc acacacagag agagactcaa acacactcac agcttcacac tgagctccaa
     4621 gagctctgta gagagaagcc agagtatgct ttcctcgatt ttcctaacgt cttcttcttc
     4681 tgcttttttc tttttcccaa cgagttgtag tccactaact tcaatttttc ctccagagaa
     4741 agagttttag agagacagct gataaataca ctagagaaat aaaaagaatg ataaaaattt
     4801 gctattttaa tcagttccct ctcgtttctc taagttttgt tgttgggttg ggaaattaaa
     4861 attggaaaat ggaaataacg tttttttctg tctaatcttg aattctccag acatttctta
     4921 tttccttttt tgttgcaagt ttttcttcct tctctaattt ttaatcaaag atatctcgct
     4981 atgaactttg aatttttgat attttgattc tataaataca taaagtaatc taaatgctct
     5041 tttaaaataa atctacagta gccctatttt cagcctagga tgtctgattc cgctgttgtt
     5101 gccgccgctg tcgagccaaa ggtcccaaag gctaaggccg ccaaggccgc caagccagcc
     5161 gccaaggccg ccaagccaac caaagttgcc aaggccaagg ctccagtcgc tcacccacca
     5221 tacatcaaca tgatcaagga ggccatcaag cagctcaagg accgcaaagg agcttccaag
     5281 caggcaatcc tcaagttcat ctcccaaaac tacaagctcg gagacaatgt catccagatc
     5341 aatgctcatc tccgtcaggc tctcaagcgt ggtgtcacca gcaaggccct tgttcaagcc
     5401 gccggatccg gagccaacgg acgtttccgt gtgccagaga aggccgccgc cgccaagaag
     5461 ccagcagcag ccaagaagcc agcagcagcc aagaaaccag cagccgccaa gaaagccact
     5521 ggagagaaga aggcaaagaa gccagccgct gccaagccaa agaaggccgc caccggagat
     5581 aagaaggtca agaaggccaa gagcccaaag aaggttgcca agccagctgc caagaaggtc
     5641 gccaagtctc cagccaagaa ggccgctcca aagaagatcg ccaagccagc tgccaagaag
     5701 gccgccaagc cagccgccaa ggccgcatgc atgggttctc atcatcatca tcatcatggt
     5761 atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag
     5821 gatctcgcca ccatggtcga ctcatcacgt cgtaagtgga ataagacagg tcacgcagtc
     5881 agagctatag gtcggctgag ctcactcgag aacgtctata tcaaggccga caagcagaag
     5941 aacggcatca aggcgaactt ccacatccgc cacaacatcg aggacggcgg cgtgcagctc
     6001 gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     6061 cactacctga gcgtgcagtc caaactttcg aaagacccca acgagaagcg cgatcacatg
     6121 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     6181 ggcggtaccg gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     6241 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt
     6301 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     6361 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     6421 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc
     6481 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     6541 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     6601 ggcaacatcc tggggcacaa gctggagtac aacctgccgg accaactgac tgaagagcag
     6661 atcgcagaat ttaaagaggc tttctcccta tttgacaagg acggggatgg gacaataaca
     6721 accaaggagc tggggacggt gatgcggtct ctggggcaga accccacaga agcagagctg
     6781 caggacatga tcaatgaagt agatgccgac ggtgacggca caatcgactt ccctgagttc
     6841 ctgacaatga tggcaagaaa aatgaaatac agggacacgg aagaagaaat tagagaagcg
     6901 ttcggtgtgt ttgataagga tggcaatggc tacatcagtg cagcagagct tcgccacgtg
     6961 atgacaaacc ttggagagaa gttaacagat gaagaggttg atgaaatgat cagggaagca
     7021 gacatcgatg gggatggtca ggtaaactac gaagagtttg tacaaatgat gacagcgaag
     7081 tgaaagcttt ggaattcatc tcgcgcccgt gcctctgact tctaagtcca attactcttc
     7141 aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa
     7201 aaacttcttc ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat
     7261 tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct
     7321 ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat
     7381 gtttttttta cttctgataa attttttttg aaacatcata gaaaaaaccg cacacaaaat
     7441 accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg
     7501 gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt
     7561 ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg ggggtttccc
     7621 ctattgtttg tcaagagttt cgaggacggc gtttttcttg ctaaaatcac aagtattgat
     7681 gagcacgatg caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt
     7741 agaagttgat aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa
     7801 tacattccca atataccaaa cataactgtt tgggcccggt acccagcttt tgttcccttt
     7861 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7921 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7981 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8041 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8101 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8161 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8221 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8281 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8341 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8401 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8461 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8521 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8581 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8641 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8701 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     8761 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8821 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8881 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8941 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9001 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9061 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9121 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9181 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9241 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9301 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9361 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9421 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9481 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9541 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9601 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9661 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9721 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9781 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9841 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9901 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9961 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10021 gcacatttcc ccgaaaagtg ccac
//
