LOCUS       pBHDuet40               4256 bp    DNA     circular UNA 01-OCT-2018
DEFINITION  gp41-1?C36.
ACCESSION   urn.local...1385710949252.151
VERSION     urn.local...1385710949252.151
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    30..46
                     /note="Geneious type: promoter prokaryotic"
                     /standard_name="T7 Promoter"
     misc_feature    51..71
                     /note="Geneious type: protein_binding"
                     /standard_name="lacO"
     RBS             104..109
                     /standard_name="RBS-1"
     CDS             117..173
                     /standard_name="6xHis and thrombine site"
     misc_feature    117..617
                     /note="Geneious type: ORF"
                     /standard_name="ORF 2 (frame 3)"
     CDS             177..344
                     /standard_name="ProteinG"
     misc_feature    353..614
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TTTAAGCTATGAAACGGAAATATTGACAGTAGAATATGGATT
                     ATTACCGATTGGTAAAATTGTAGAAAAGCGCATCGAATGTACTGTTTATAGCGTTGAT
                     AATAATGGAAATATTTATACACAACCTGTAGCACAATGGCACGATCGCGGAGAACAAG
                     AGGTGTTTGAGTATTGTTTGGAAGATGGTTCATTGATTCGGGCAACAAAAGACCATAA
                     GTTTATGACTGTTGATGGTCAAATGTTGCCAATTGATGAAATATTTGAACGTGAATTG
                     GATTTGATGCGGGTTGATAATTTGCCGAAT"
     misc_feature    687..703
                     /note="Geneious type: promoter prokaryotic"
                     /standard_name="T7 promoter"
     misc_feature    708..728
                     /note="Geneious type: protein_binding"
                     /standard_name="lacO"
     RBS             759..764
     CDS             773..901
                     /standard_name="MCS-2 ORF"
     misc_feature    839..883
                     /note="Geneious type: protein_binding"
                     /standard_name="S-Tag"
     misc_feature    complement(921..939)
                     /note="Geneious type: primer"
                     /standard_name="T7 Terminator primer"
     terminator      935..982
                     /standard_name="T7 Terminator"
     CDS             complement(1212..2027)
                     /standard_name="Kan"
     RBS             complement(2033..2037)
     -10_signal      complement(2069..2075)
                     /standard_name="-10 Ap"
     -35_signal      complement(2092..2097)
                     /standard_name="-35 Ap"
     rep_origin      2135..2884
     misc_RNA        complement(2135..2833)
                     /standard_name="RNA II"
     -35_signal      2688..2693
                     /standard_name="-35 RNA I"
     -10_signal      2712..2717
                     /standard_name="-10 RNA I"
     misc_RNA        2724..2830
                     /standard_name="RNA I"
     CDS             complement(3080..4162)
                     /standard_name="lacI"
ORIGIN      
        1 tcccttatgc gactcctgca ttaggaaatt aatacgactc actatagggg aattgtgagc
       61 ggataacaat tcccctgtag aaataatttt gtttaacttt aataaggaga tataccatgg
      121 gcagcagcca tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgc
      181 agtacaaact tatcctgaac ggtaaaaccc tgaaaggtga aaccaccacc gaagctgttg
      241 acgctgctac cgcggaaaaa gttttcaaac agtacgctaa cgacaacggt gttgacggtg
      301 aatggaccta cgacgacgct accaaaacct acaccgtaac ggaaggatcc tgcctggatc
      361 tgaaaacgca ggtgcagacc ccacagggca tgaaagagat atccaacatt caggtgggcg
      421 atcttgttct gagcaacacc ggctataacg aggtgctgaa cgtgtttccg aaaagcaaaa
      481 agaaaagcta taaaattacc ctggaagatg gcaaagaaat tatttgtagc gaagaacatc
      541 tgttcccgac ccagaccggc gaaatgaaca tcagcggcgg tctgaaagag ggcatgtgcc
      601 tgtatgtgaa agaataagct tgcggccgca taatgcttaa gtcgaacaga aagtaatcgt
      661 attgtacacg gccgcataat cgaaattaat acgactcact ataggggaat tgtgagcgga
      721 taacaattcc ccatcttagt atattagtta agtataagaa ggagatatac atatggcaga
      781 tctcaattgg atatcggccg gccacgcgat cgctgacgtc ggtaccctcg agtctggtaa
      841 agaaaccgct gctgcgaaat ttgaacgcca gcacatggac tcgtctacta gcgcagctta
      901 attaacctag gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
      961 acgggtcttg aggggttttt tgctgaaacc tcaggcattt gagaagcaca cggtcacact
     1021 gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc
     1081 ctgccctgaa ccgacgacaa gctgacgacc gggtctccgc aagtggcact tttcggggaa
     1141 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1201 tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     1261 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     1321 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     1381 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     1441 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
     1501 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     1561 attcgtgatt gcgcctgagc gagacgaaat acgcggtcgc tgttaaaagg acaattacaa
     1621 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     1681 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     1741 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     1801 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     1861 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     1921 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     1981 tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcatact cttccttttt
     2041 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2101 atttagaaaa ataaacaaat aggcatgcag cgctcttccg cttcctcgct cactgactcg
     2161 ctacgctcgg tcgttcgact gcggcgagcg gtgtcagctc actcaaaagc ggtaatacgg
     2221 ttatccacag aatcagggga taaagccgga aagaacatgt gagcaaaaag caaagcaccg
     2281 gaagaagcca acgccgcagg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2341 aaatcgacgc tcaagccaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2401 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2461 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gttggtatct
     2521 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2581 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2641 atcgccactg gcagcagcca ttggtaactg atttagagga ctttgtcttg aagttatgca
     2701 cctgttaagg ctaaactgaa agaacagatt ttggtgagtg cggtcctcca acccacttac
     2761 cttggttcaa agagttggta gctcagcgaa ccttgagaaa accaccgttg gtagcggtgg
     2821 tttttcttta tttatgagat gatgaatcaa tcggtctatc aagtcaacga acagctattc
     2881 cgttactcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
     2941 tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga aggagctgac
     3001 tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact
     3061 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     3121 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg
     3181 tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag
     3241 agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg
     3301 tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga
     3361 tgtccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct
     3421 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt
     3481 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat
     3541 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg
     3601 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc
     3661 gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa
     3721 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca
     3781 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt
     3841 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
     3901 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg
     3961 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa
     4021 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc
     4081 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat
     4141 cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc
     4201 atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctc
//
