LOCUS       pTU-C-000     3388 bp ds-DNA     circular     08-MAR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    149..156
                     /label="NotI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    113..118
                     /label="AatII"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    95..100
                     /label="SalI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    277..283
                     /label="BbvCI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    265..276
                     /label="Spacer 15"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    101..112
                     /label="Spacer 5"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    61..66
                     /label="EcoRI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    214..258
                     /label="Linker 8"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    67..94
                     /label="Truncated (3') Spacer 2.5"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    284..291
                     /label="AscI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    119..148
                     /label="Linker_11"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    157..213
                     /label="ECK120033737 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     oriT            complement(1684..2303)
                     /label="pBR322 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     gene            complement(2461..3318)
                     /label="AmpR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    259..264
                     /label="KasI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
       61 GAATTCAGAA GTATTGGTAA TCGTTGAAAA CTCAGTCGAC GCACTTACTG AAGACGTCCT
      121 ATTACACTCG TCGTTGGAAA CTGAAGATGC GGCCGCggaa acacagAAAA AAGCCCGCAC
      181 CTGACAGTGC GGGCTTTTTT TTTcgaccaa aggTAGCGAA CGACGAGTCA CTGTTGAGGA
      241 TAAATACTTT CTCTACTAGG CGCCTGTTAC ACAGGTCCTC AGCGGCGCGC CTGCTGCCAC
      301 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT
      361 GCCAGGCATC AAATAAAACG AAAGGCTCAG TCGGAAGACT GGGCCTTTCG TTTTATCTGT
      421 TGTTTGTCGG TGAACGCTCT CCTGAGTAGG ACAAATCCGC CGGGAGCGGA TTTGAACGTT
      481 GTGAAGCAAC GGCCCGGAGG GTGGCGGGCA GGACGCCCGC CATAAACTGC CAGGCATCAA
      541 ACTAAGCAGA AGGCCATCCT GACGGATGGC CTTTTTGCGT TTCagatctc gggcagcgtt
      601 gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg
      661 cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
      721 tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt
      781 tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc
      841 gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat
      901 tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca
      961 caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct
     1021 cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg
     1081 accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg
     1141 cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt
     1201 cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa
     1261 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     1321 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     1381 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     1441 ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     1501 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1561 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1621 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1681 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1741 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1801 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1861 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1921 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1981 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2041 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2101 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2161 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2221 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2281 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2341 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2401 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2461 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2521 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2581 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2641 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2701 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2761 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2821 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2881 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2941 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3001 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3061 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3121 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3181 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3241 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3301 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3361 ttgtctcatg agcggataca tatttgaa
//