LOCUS       pTU-A-005     4674 bp ds-DNA     circular     08-MAR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    97..102
                     /label="SacI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            complement(3747..4604)
                     /label="AmpR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    67..96
                     /label="Linker_14"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1372..1571
                     /label="Spacer 1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    115..120
                     /label="XhoI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    874..1260
                     /label="MarA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    151..165
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    103..114
                     /label="Spacer 8"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1261..1275
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    61..66
                     /label="EcoRI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    186..219
                     /label="RBS"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    1572..1577
                     /label="HindIII"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1282..1371
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    121..150
                     /label="Linker_0"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    220..873
                     /label="mKO2"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     oriT            complement(2970..3589)
                     /label="pBR322 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1276..1281
                     /label="NcoI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    166..185
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
       61 GAATTCATAG GATAGATTCT GGAAACTTTA CCGTCCGAGC TCAGGCTTAC CTTACTCGAG
      121 CAATAAACAG TTGATAGGGC TTCTCCGTTA CAGCCTGCGG TCCGGGTTCA CTGCCGTATA
      181 GGCAGTAATT TTGTTTAACT TTAAGAAGGA GATATACATA TGGTTTCGGT TATCAAACCA
      241 GAGATGAAAA TGCGTTACTA TATGGATGGT TCAGTAAATG GTCACGAATT TACTATTGAG
      301 GGCGAGGGTA CGGGACGCCC ATACGAGGGG CACCAGGAAA TGACTTTACG CGTCACAATG
      361 GCTGAAGGCG GGCCTATGCC GTTTGCGTTC GATCTTGTTA GTCATGTCTT TTGTTACGGT
      421 CACCGTGTAT TTACTAAATA CCCCGAGGAA ATTCCAGACT ATTTCAAACA AGCCTTCCCG
      481 GAAGGTTTGT CTTGGGAGCG CAGTTTAGAG TTTGAAGACG GTGGCTCGGC CAGCGTGTCA
      541 GCTCATATTA GTCTTCGCGG CAATACATTT TATCACAAGT CAAAGTTCAC CGGCGTGAAC
      601 TTCCCCGCAG ACGGCCCAAT CATGCAGAAT CAAAGTGTTG ATTGGGAACC GTCCACAGAG
      661 AAGATTACAG CTTCCGATGG AGTCTTAAAG GGCGATGTAA CCATGTACTT AAAATTAGAA
      721 GGGGGAGGGA ACCATAAATG TCAGATGAAG ACTACCTATA AGGCCGCAAA AGAGATTCTT
      781 GAAATGCCCG GAGACCACTA CATTGGGCAT CGTTTGGTCC GTAAGACAGA AGGAAATATT
      841 ACTGAACAGG TCGAAGACGC TGTGGCACAC AGCATGTCCC GCCGTAATAC TGACGCCATC
      901 ACAATCCACA GCATCCTGGA TTGGATTGAA GACAACTTGG AGTCGCCGTT GAGTTTAGAA
      961 AAAGTTAGTG AACGTAGTGG TTACTCAAAG TGGCACCTTC AGCGCATGTT TAAGAAGGAA
     1021 ACGGGTCATT CATTGGGTCA ATATATTCGT TCTCGCAAGA TGACTGAAAT TGCCCAGAAA
     1081 TTGAAAGAGT CTAATGAACC TATTTTGTAC CTGGCGGAGC GTTACGGCTT TGAAAGTCAG
     1141 CAAACCCTTA CACGTACCTT CAAGAATTAC TTTGACGTTC CACCACACAA ATATCGTATG
     1201 ACCAACATGC AGGGTGAGTC ACGTTTTTTG CATCCGTTGA ATCATTACAA TTCCTAATAA
     1261 TCGCTGGGAC GCCCGCCATG GTTCAGCCAA AAAACTTAAG ACCGCCGGTC TTGTCCACTA
     1321 CCTTGCAGTA ATGCGGTGGA CAGGATCGGC GGTTTTCTTT TCTCTTCTCA ATTCTTCTGA
     1381 CCTGTAACGA ATAATAGATA GTAAAGTAGT CTCCGATTGA GTTTTCTCTG CCGAGTCCCA
     1441 CCCAGTTCTG TGATTTCAGT AAGTTGGTAA TTGATACACT GTTGCGAGAA CTGCTGCCTG
     1501 GTAGTAGATA GGTTGTTATT GAGTAAGAAG GTAAAGTGAA CGAAATCCCT GAAACTGAGA
     1561 CTGTAGAAAA TAAGCTTTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
     1621 TCTAAACGGG TCTTGAGGGG TTTTTTGCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGG
     1681 AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA
     1741 ATCCGCCGGG AGCGGATTTG AACGTTGTGA AGCAACGGCC CGGAGGGTGG CGGGCAGGAC
     1801 GCCCGCCATA AACTGCCAGG CATCAAACTA AGCAGAAGGC CATCCTGACG GATGGCCTTT
     1861 TTGCGTTTCa gatctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc
     1921 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
     1981 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
     2041 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct
     2101 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta
     2161 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca
     2221 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag
     2281 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa
     2341 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc
     2401 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
     2461 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc
     2521 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     2581 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     2641 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     2701 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat gcggtgtgaa
     2761 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     2821 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2881 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2941 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3001 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3061 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3121 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3181 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3241 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3301 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3361 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3421 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3481 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3541 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3601 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3661 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3721 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3781 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3841 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3901 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3961 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4021 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
     4081 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4141 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4201 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4261 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4321 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     4381 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4441 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4501 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4561 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4621 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaa
//