LOCUS       pC-0_v3        5406 bp ds-DNA     circular     08-MAR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     oriT            complement(2613..3232)
                     /label="pBR322 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    707..718
                     /label="Spacer 7"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    411..500
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    315..320
                     /label="EcoRI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    725..754
                     /label="Linker_10"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1143..1187
                     /label="Linker 8"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    351..356
                     /label="SacI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1086..1142
                     /label="ECK120033737 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    501..700
                     /label="Spacer 1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     gene            complement(3390..4247)
                     /label="AmpR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        274..302
                     /label="P(BAD) promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    755..762
                     /label="SbfI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1030..1041
                     /label="Spacer 5"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1024..1029
                     /label="SalI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1078..1085
                     /label="NotI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    357..368
                     /label="Spacer 8"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1042..1047
                     /label="AatII"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            complement(4528..5406)
                     /label="araC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    824..1023
                     /label="Spacer 2.5"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    701..706
                     /label="HindIII"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    763..823
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    375..404
                     /label="Linker_0"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1188..1193
                     /label="KasI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    719..724
                     /label="BamHI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    321..350
                     /label="Linker_14"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    369..374
                     /label="XhoI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1194..1205
                     /label="Spacer 15"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1048..1077
                     /label="Linker_11"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    405..410
                     /label="NcoI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1213..1220
                     /label="AscI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1206..1212
                     /label="BbvCI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ACTTTTCATA CTCCCGCCAT TCAGAGAAGA AACCAATTGT CCATATTGCA TCAGACATTG
       61 CCGTCACTGC GTCTTTTACT GGCTCTTCTC GCTAACCAAA CCGGTAACCC CGCTTATTAA
      121 AAGCATTCTG TAACAAAGCG GGACCAAAGC CATGACAAAA ACGCGTAACA AAAGTGTCTA
      181 TAATCACGGC AGAAAAGTCC ACATTGATTA TTTGCACGGC GTCACACTTT GCTATGCCAT
      241 AGCATTTTTA TCCATAAGAT TAGCGGtTCC TACCTGACGC TTTTTATCGC AACTCTCTAC
      301 TGTTTCTCCA TACCGAATTC ATAGGATAGA TTCTGGAAAC TTTACCGTCC GAGCTCAGGC
      361 TTACCTTACT CGAGCAATAA ACAGTTGATA GGGCTTCTCC GTTACCATGG TTCAGCCAAA
      421 AAACTTAAGA CCGCCGGTCT TGTCCACTAC CTTGCAGTAA TGCGGTGGAC AGGATCGGCG
      481 GTTTTCTTTT CTCTTCTCAA TTCTTCTGAC CTGTAACGAA TAATAGATAG TAAAGTAGTC
      541 TCCGATTGAG TTTTCTCTGC CGAGTCCCAC CCAGTTCTGT GATTTCAGTA AGTTGGTAAT
      601 TGATACACTG TTGCGAGAAC TGCTGCCTGG TAGTAGATAG GTTGTTATTG AGTAAGAAGG
      661 TAAAGTGAAC GAAATCCCTG AAACTGAGAC TGTAGAAAAT AAGCTTGTCC AGACTATTGG
      721 ATCCAAGAGA TTTCTACACG ATTGAGCACT GTCTCCTGCA GGCTCGGTAC CAAATTCCAG
      781 AAAAGAGGCC TCCCGAAAGG GGGGCCTTTT TTCGTTTTGG TCCTAATAGA TAAAGGATAG
      841 GTCTGGTAGT GTTGTTCGTT CTCGCAGGTA AATCAATAAT ACTCAGCAGT TCCGTAGACT
      901 TTTCAGTGGG ACAGGGTAGC GATAACAGAT AGATTGTAAT AAGACACAGT AGGTGCTCGT
      961 AGTTGCGTGA AGAGAACCGC TCAGGAAATC CAGTCAGAAG TATTGGTAAT CGTTGAAAAC
     1021 TCAGTCGACG CACTTACTGA AGACGTCCTA TTACACTCGT CGTTGGAAAC TGAAGATGCG
     1081 GCCGCggaaa cacagAAAAA AGCCCGCACC TGACAGTGCG GGCTTTTTTT TTcgaccaaa
     1141 ggTAGCGAAC GACGAGTCAC TGTTGAGGAT AAATACTTTC TCTACTAGGC GCCTGTTACA
     1201 CAGGTCCTCA GCGGCGCGCC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     1261 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CCAGGCATCA AATAAAACGA AAGGCTCAGT
     1321 CGGAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC CTGAGTAGGA
     1381 CAAATCCGCC GGGAGCGGAT TTGAACGTTG TGAAGCAACG GCCCGGAGGG TGGCGGGCAG
     1441 GACGCCCGCC ATAAACTGCC AGGCATCAAA CTAAGCAGAA GGCCATCCTG ACGGATGGCC
     1501 TTTTTGCGTT TCagatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc
     1561 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
     1621 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
     1681 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc
     1741 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac
     1801 ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc
     1861 gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat
     1921 cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca
     1981 gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg
     2041 cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg
     2101 atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct
     2161 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     2221 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     2281 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     2341 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt
     2401 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     2461 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2521 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2581 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2641 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2701 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2761 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2821 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2881 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2941 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3001 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3061 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3121 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3181 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3241 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3301 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3361 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3421 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3481 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3541 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3601 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3661 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     3721 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3781 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3841 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3901 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3961 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     4021 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4081 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4141 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4201 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4261 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4321 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccaccttca
     4381 ctctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga
     4441 tataagttgt aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt
     4501 tgCTCCTAgG GTCTGATTCG TTACCAATTA TGACAACTTG ACGGCTACAT CATTCACTTT
     4561 TTCTTCACAA CCGGCACGGA ACTCGCTCGG GCTGGCCCCG GTGCATTTTT TAAATACCCG
     4621 CGAGAAATAG AGTTGATCGT CAAAACCAAC ATTGCGACCG ACGGTGGCGA TAGGCATCCG
     4681 GGTGGTGCTC AAAAGCAGCT TCGCCTGGCT GATACGTTGG TCCTCGCGCC AGCTTAAGAC
     4741 GCTAATCCCT AACTGCTGGC GGAAAAGATG TGACAGACGC GACGGCGACA AGCAAACATG
     4801 CTGTGCGACG CTGGCGATAT CAAAATTGCT GTCTGCCAGG TGATCGCTGA TGTACTGACA
     4861 AGCCTCGCGT ACCCGATTAT CCATCGGTGG ATGGAGCGAC TCGTTAATCG CTTCCATGCG
     4921 CCGCAGTAAC AATTGCTCAA GCAGATTTAT CGCCAGCAGC TCCGAATAGC GCCCTTCCCC
     4981 TTGCCCGGCG TTAATGATTT GCCCAAACAG GTCGCTGAAA TGCGGCTGGT GCGCTTCATC
     5041 CGGGCGAAAG AACCCCGTAT TGGCAAATAT TGACGGCCAG TTAAGCCATT CATGCCAGTA
     5101 GGCGCGCGGA CGAAAGTAAA CCCACTGGTG ATACCATTCG CGAGCCTCCG GATGACGACC
     5161 GTAGTGATGA ATCTCTCCTG GCGGGAACAG CAAAATATCA CCCGGTCGGC AAACAAATTC
     5221 TCGTCCCTGA TTTTTCACCA CCCCCTGACC GCGAATGGTG AGATTGAGAA TATAACCTTT
     5281 CATTCCCAGC GGTCGGTCGA TAAAAAAATC GAGATAACCG TTGGCCTCAA TCGGCGTTAA
     5341 ACCCGCCACC AGATGGGCAT TAAACGAGTA TCCCGGCAGC AGGGGATCAT TTTGCGCTTC
     5401 AGCCAT
//