LOCUS       004_pCS2_GFP1_9         4683 bp ds-DNA     circular     03-MAR-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     misc_feature    79..84
                     /label=Polylinker I
                     /ApEinfo_fwdcolor=#ff3c86
                     /ApEinfo_revcolor=#ff3c86
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_site      732..927
                     /label=PolyA
                     /ApEinfo_fwdcolor=#0509ff
                     /ApEinfo_revcolor=#0509ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    961..997
                     /label=Polylinker II
                     /ApEinfo_fwdcolor=#ff0388
                     /ApEinfo_revcolor=#ff0388
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        4488..4683
                     /label=CMV promoter
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    981..997
                     /label=T3
                     /ApEinfo_fwdcolor=#fff90e
                     /ApEinfo_revcolor=#fff90e
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    35..51
                     /label=Sp6
                     /ApEinfo_fwdcolor=#ffef13
                     /ApEinfo_revcolor=#ffef13
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    711..727
                     /label=T7
                     /ApEinfo_fwdcolor=#fff01e
                     /ApEinfo_revcolor=#fff01e
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    682..708
                     /label=Polylinker I(1)
                     /ApEinfo_label=Polylinker I
                     /ApEinfo_fwdcolor=#ff3c86
                     /ApEinfo_revcolor=#ff3c86
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    91..678
                     /label=GFP1-9
                     /ApEinfo_fwdcolor=#80ff80
                     /ApEinfo_revcolor=#80ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
       61 CTACTTGTTC TTTTTGCAGG ATCCGCCACC ATGCGCAAAG GCGAAGAACT GTTTACCGGC
      121 ATTGTGCCGA TTCTGGTGGA ACTGGATGGC GATGTGAACG GCCATAAATT TTTTGTGCGC
      181 GGCGAAGGCG AAGGCGATGC GACCATTGGC AAACTGAGCC TGAAATTTAT TTGCACCACC
      241 GGCAAACTGC CGGTGCCGTG GCCGACCCTG GTGACCACCC TGACCTATGG CGTGCAGTGC
      301 TTTAGCCGCT ATCCGGATCA TATGAAACGC CATGATTTTT TTAAAAGCGC GATGCCGGAA
      361 GGCTATGTGC AGGAACGCAC CATTTATTTT AAAGATGATG GCACCTATAA AACCCGCGCG
      421 GAAGTGAAAT TTGAAGGCGA TACCCTGGTG AACCGCATTG AACTGAAAGG CATTGATTTT
      481 AAAGAAGATG GCAACATTCT GGGCCATAAA CTGGAATATA ACTTTAACAG CCATAAAGTG
      541 TATATTACCG CGGATAAACA GAACAACGGC ATTAAAGCGA ACTTTACCAT TCGCCATAAC
      601 GTGGAAGATG GCAGCGTGCA GCTGGCGGAT CATTATCAGC AGAACACCCC GATTGGCGAT
      661 GGCCCGGTTC TTCTTCCTTA AGAATTCAAG GCCTCTCGAG CCTCTAGAAC TATAGTGAGT
      721 CGTATTACGT AGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA
      781 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA
      841 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
      901 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAT TCGCGGCCGC GGCGCCAATG CATTGGGCCC
      961 GGTACCCAGC TTTTGTTCCC TTTAGTGAGG GTTAATTGCG CGCTTGGCGT AATCATGGTC
     1021 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG
     1081 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT
     1141 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG
     1201 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA
     1261 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT
     1321 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA
     1381 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC
     1441 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1501 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1561 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1621 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1681 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1741 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1801 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1861 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1921 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1981 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     2041 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT
     2101 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA
     2161 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     2221 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     2281 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC
     2341 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     2401 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     2461 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC
     2521 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     2581 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     2641 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     2701 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     2761 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     2821 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     2881 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     2941 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     3001 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA
     3061 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     3121 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTA AATTGTAAGC
     3181 GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA
     3241 TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT
     3301 GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG
     3361 CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT
     3421 TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA
     3481 GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG
     3541 GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG
     3601 CTTAATGCGC CGCTACAGGG CGCGTCCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA
     3661 GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGTCGA CCATAGCCAA TTCAATATGG
     3721 CGTATATGGA CTCATGCCAA TTCAATATGG TGGATCTGGA CCTGTGCCAA TTCAATATGG
     3781 CGTATATGGA CTCGTGCCAA TTCAATATGG TGGATCTGGA CCCCAGCCAA TTCAATATGG
     3841 CGGACTTGGC ACCATGCCAA TTCAATATGG CGGACTTGGC ACTGTGCCAA CTGGGGAGGG
     3901 GTCTACTTGG CACGGTGCCA AGTTTGAGGA GGGGTCTTGG CCCTGTGCCA AGTCCGCCAT
     3961 ATTGAATTGG CATGGTGCCA ATAATGGCGG CCATATTGGC TATATGCCAG GATCAATATA
     4021 TAGGCAATAT CCAATATGGC CCTATGCCAA TATGGCTATT GGCCAGGTTC AATACTATGT
     4081 ATTGGCCCTA TGCCATATAG TATTCCATAT ATGGGTTTTC CTATTGACGT AGATAGCCCC
     4141 TCCCAATGGG CGGTCCCATA TACCATATAT GGGGCTTCCT AATACCGCCC ATAGCCACTC
     4201 CCCCATTGAC GTCAATGGTC TCTATATATG GTCTTTCCTA TTGACGTCAT ATGGGCGGTC
     4261 CTATTGACGT ATATGGCGCC TCCCCCATTG ACGTCAATTA CGGTAAATGG CCCGCCTGGC
     4321 TCAATGCCCA TTGACGTCAA TAGGACCACC CACCATTGAC GTCAATGGGA TGGCTCATTG
     4381 CCCATTCATA TCCGTTCTCA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCACTTG
     4441 GCAGTACATC AATATCTATT AATAGTAACT TGGCAAGTAC ATTACTATTG GAAGGACGCC
     4501 AGGGTACATT GGCAGTACTC CCATTGACGT CAATGGCGGT AAATGGCCCG CGATGGCTGC
     4561 CAAGTACATC CCCATTGACG TCAATGGGGA GGGGCAATGA CGCAAATGGG CGTTCCATTG
     4621 ACGTAAATGG GCGGTAGGCG TGCCTAATGG GAGGTCTATA TAAGCAATGC TCGTTTAGGG
     4681 AAC     
//
