LOCUS pSB700_HL1gR4 9063 bp ds-DNA circular 19-FEB-2019 DEFINITION . ACCESSION VERSION . KEYWORDS pSB700 lenti gRNA Cerulean. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 9061) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Feb 11, 2014 from SnapGene Viewer 1.5.3 http://www.snapgene.com COMMENT LOCUS pLVX-ZsGreen1-N1 8763 bp DNA circular 7-OCT-2009 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No. 632565 ApEinfo:methylated:1. FEATURES Location/Qualifiers LTR 1..635 /note="5'\LTR" misc_binding 636..653 /note="PBS" misc_feature 685..822 /note="packaging\signal" misc_binding 1303..1536 /note="RRE" misc_feature 2028..2151 /note="cPPT/CTS\(DNA\flap)" promoter 2246..2494 /note="Human U6 Promoter" misc_feature 2465..2490 /note="User" misc_feature 2494^2495 /note="Geneious type: Editing History Deletion" misc_feature 2496..2515 /note="Geneious type: Editing History Insertion" misc_RNA 2516..2598 /note="User" /note="sgRNA backbone" misc_feature complement(2600..2624) /note="User" enhancer 2669..2956 /note="""CAG enhancer"" H CMV IE enhancer like" /note="""CAG enhancer"" H CMV IE enhancer like pCAGGS" promoter 2975..4215 /note="CAG promoter like pCAGGS" misc_feature complement(4270..4290) /note="CAGGS cloning primer" misc_feature 4271..4290 /note="pCAG_F_primer from Addgene" misc_feature 4291..4296 /note="Kozak" CDS 4297..4969 CDS 4971..5007 gene 5008..5018 /note="EGFP" misc_feature 5031..5622 /note="WPRE" LTR 5825..6461 /note="3'\LTR" misc_feature 6637..6666 /note="lac promoter" rep_origin complement(6931..7604) CDS complement(7749..8745) /note="Amp(R) with promoter 7449-8379" misc_feature 8765..8896 /note="SV40 polyA" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cattagtgaa cggatctcga cggtatcgat cacgagacta 2221 gcctcgagcg gccgccccct tcaccgaggg cctatttccc atgattcctt catatttgca 2281 tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat 2341 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa 2401 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt 2461 ggctttatat atcttgtgga aaggacgaaa caccgattct accagaggta caagggtttt 2521 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac 2581 cgagtcggtg ctttttttga attctcgacc tcgagacaaa tggcagtatt cgtcattagt 2641 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 2701 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 2761 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 2821 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 2881 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 2941 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct 3001 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg 3061 tgcagcgatg ggggcggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg 3121 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa 3181 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg 3241 ggcggggagt cgctgcgacg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc 3301 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct 3361 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg 3421 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt 3481 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt 3541 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg 3601 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg 3661 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc 3721 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg 3781 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc 3841 ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc 3901 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag 3961 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc 4021 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc 4081 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc 4141 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg 4201 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta 4261 cagctcctgg gcaacgtgct ggttattgtg gccaccatgg tgagcaaggg cgaagaagat 4321 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 4381 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 4441 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 4501 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 4561 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 4621 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 4681 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 4741 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 4801 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 4861 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 4921 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggcca ccactccacc 4981 ggcggcatgg acgagctgta caagtaatgt acaagtaaag cgtctggaac aatcaacctc 5041 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 5101 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 5161 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 5221 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 5281 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 5341 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 5401 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 5461 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 5521 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 5581 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg 5641 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg 5701 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc 5761 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag 5821 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta 5881 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata 5941 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga 6001 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga 6061 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc 6121 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact 6181 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct 6241 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga 6301 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 6361 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 6421 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 6481 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt 6541 gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 6601 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 6661 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 6721 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 6781 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 6841 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 6901 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 6961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 7021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 7081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 7141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 7201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 7261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 7321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 7381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 7441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 7501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 7561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 7621 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 7681 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 7741 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 7801 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 7861 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 7921 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 7981 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 8041 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 8101 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 8161 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 8221 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 8281 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 8341 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 8401 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 8461 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 8521 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 8581 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 8641 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 8701 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat 8761 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 8821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 8881 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac 8941 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc 9001 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt 9061 agt //