LOCUS       nifF_in_pET21b          5950 bp ds-DNA     circular     25-JAN-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     Gene            1201..1743
                     /label="nifF (Flavodoxin II)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             2743..3402
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      1975..2281
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      3500..4182
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      1958..2413
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(1860..1878)
                     /label="T7 Reverse"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1790..1807
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_binding    1132..1154
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     1113..1132
                     /label="T7 Forward"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat
       61 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt
      121 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc
      181 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg
      241 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac
      301 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat
      361 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc
      421 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat
      481 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag
      541 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc
      601 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac
      661 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg
      721 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa
      781 ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca
      841 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg
      901 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
      961 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     1021 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     1081 cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg
     1141 agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat
     1201 ATGGCGAAAA TCGGCCTGTT CTTTGGCAGC AATACCGGCA AGACCCGTAA GGTGGCGAAG
     1261 AGCATTAAAA AACGCTTTGA CGACGAAACC ATGAGCGACG CGCTGAACGT GAACCGTGTT
     1321 AGCGCGGAGG ATTTCGCGCA GTACCAATTT CTGATTCTGG GCACCCCGAC CCTGGGCGAG
     1381 GGTGAACTGC CGGGCCTGAG CAGCGATTGC GAGAACGAAA GCTGGGAGGA GTTCCTGCCG
     1441 AAGATTGAAG GTCTGGACTT CAGCGGCAAA ACCGTGGCGC TGTTTGGTCT GGGCGACCAG
     1501 GTTGGTTACC CGGAGAACTA TCTGGATGCG CTGGGCGAAC TGTACAGCTT CTTTAAGGAC
     1561 CGTGGTGCGA AAATCGTGGG CAGCTGGAGC ACCGATGGTT ATGAGTTCGA AAGCAGCGAG
     1621 GCGGTGGTTG ACGGCAAGTT TGTTGGCCTG GCGCTGGACC TGGATAACCA AAGCGGCAAA
     1681 ACCGATGAGC GTGTGGCGGC GTGGCTGGCG CAAATCGCGC CGGAGTTCGG TCTGAGCCTG
     1741 TAAggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca
     1801 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     1861 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     1921 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
     1981 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2041 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2101 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2161 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2221 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     2281 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     2341 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     2401 acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     2461 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2521 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     2581 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2641 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2701 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2761 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2821 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2881 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2941 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3001 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3061 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     3121 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3181 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3241 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     3301 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3361 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     3421 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     3481 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3541 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3601 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3661 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3721 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3781 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3841 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3901 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3961 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4021 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4081 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4141 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4201 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4261 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4321 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     4381 tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat
     4441 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     4501 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     4561 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     4621 acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc
     4681 tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct
     4741 gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta
     4801 agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat
     4861 acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc
     4921 ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa
     4981 tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat
     5041 aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac
     5101 cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc
     5161 gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct
     5221 caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcga taatggcctg
     5281 cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa
     5341 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
     5401 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
     5461 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
     5521 gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt
     5581 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5641 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc
     5701 ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt
     5761 gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta
     5821 acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatatccg
     5881 caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt
     5941 tggcaaccag
//