LOCUS pX330_NL_DHFR_Sp 9502 bp ds-DNA linear 17-APR-2019 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 6468..6515 /locus_tag="NLS tag" /ApEinfo_fwdcolor="#69a3ff" /ApEinfo_revcolor="#0000ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 6516..6518 /locus_tag="Stop codon" /ApEinfo_fwdcolor="#333333" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2565..2567 /locus_tag="GS linker (4)" /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="#00ff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3057..6467 /locus_tag="SpCas9" /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="#ffff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1869..2558 /locus_tag="SpCas9(1)" /ApEinfo_label="SpCas9" /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="#ffff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2571..3044 /locus_tag="loop 231 DHFR" /ApEinfo_fwdcolor="#ff0080" /ApEinfo_revcolor="#ff0080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1377..1850 /locus_tag="N-term DHFR DD" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3045..3056 /locus_tag="GS linker" /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="#00ff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1851..1868 /locus_tag="GS linker(1)" /ApEinfo_label="GS linker" /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#ff8000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2559..2561 /locus_tag="GS linker (4)(1)" /ApEinfo_label="GS linker (4)" /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="#00ff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2562..2564 /locus_tag="GS linker (4)(2)" /ApEinfo_label="GS linker (4)" /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="#00ff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2568..2570 /locus_tag="GS linker(2)" /ApEinfo_label="GS linker" /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="#00ff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1323..1376 /locus_tag="NLS" /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#0080ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1320..1322 /locus_tag="Start" /ApEinfo_fwdcolor="#333333" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA 241 CGAAACACCg gGTCTTCgaG AAGACctgtt ttagagctaG AAAtagcaag ttaaaataag 301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcTTTTTTg ttttagagct 361 agaaatagca agttaaaata aggctagtcc gtTTTTagcg cgtgcgccaa ttctgcagac 421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc 541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tGtgcccagt 661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac 781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg 841 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga 901 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc 961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc 1021 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg 1081 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag 1141 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac 1201 ctggagcacc tgcctgaaat cacttttttt caggttGGAC CGGTGCCACC ATGGACTATA 1261 AGGACCACGA CGGAGACTAC AAGGATCATG ATATTGATTA CAAAGACGAT GACGATAAGA 1321 TGGCCCCAAA GAAGAAGCGG AAGGTCGGTA TCCACGGAGT CCCAGCAGCC GGATCCATCA 1381 GTCTGATTGC GGCGTTAGCG GTAGATTACG TTATCGGCAT GGAAAACGCC ATGCCGTGGA 1441 ACCTGCCTGC CGATCTCGCC TGGTTTAAAC GCAACACCTT AAATAAACCC GTGATTATGG 1501 GCCGCCATAC CTGGGAATCA ATCGGTCGTC CGTTGCCAGG ACGCAAAAAT ATTATCCTCA 1561 GCAGTCAACC GAGTACGGAC GATCGCGTAA CGTGGGTGAA GTCGGTGGAT GAAGCCATCG 1621 CGGCGTGTGG TGACGTACCA GAAATCATGG TGATTGGCGG CGGTCGCGTT ATTGAACAGT 1681 TCTTGCCAAA AGCGCAAAAA CTGTATCTGA CGCATATCGA CGCAGAAGTG GAAGGCGACA 1741 CCCATTTCCC GGATTACGAG CCGGATGACT GGGAATCGGT ATTCAGCGAA TTCCACGATG 1801 CTGATGCGCA GAACTCTCAC AGCTATTGCT TTGAGATTCT GGAGCGGCGA GGAAGCGGAA 1861 GCGGATCCGA CAAGAAGTAC AGCATCGGCC TGGACATCGG CACCAACTCT GTGGGCTGGG 1921 CCGTGATCAC CGACGAGTAC AAGGTGCCCA GCAAGAAATT CAAGGTGCTG GGCAACACCG 1981 ACCGGCACAG CATCAAGAAG AACCTGATCG GAGCCCTGCT GTTCGACAGC GGCGAAACAG 2041 CCGAGGCCAC CCGGCTGAAG AGAACCGCCA GAAGAAGATA CACCAGACGG AAGAACCGGA 2101 TCTGCTATCT GCAAGAGATC TTCAGCAACG AGATGGCCAA GGTGGACGAC AGCTTCTTCC 2161 ACAGACTGGA AGAGTCCTTC CTGGTGGAAG AGGATAAGAA GCACGAGCGG CACCCCATCT 2221 TCGGCAACAT CGTGGACGAG GTGGCCTACC ACGAGAAGTA CCCCACCATC TACCACCTGA 2281 GAAAGAAACT GGTGGACAGC ACCGACAAGG CCGACCTGCG GCTGATCTAT CTGGCCCTGG 2341 CCCACATGAT CAAGTTCCGG GGCCACTTCC TGATCGAGGG CGACCTGAAC CCCGACAACA 2401 GCGACGTGGA CAAGCTGTTC ATCCAGCTGG TGCAGACCTA CAACCAGCTG TTCGAGGAAA 2461 ACCCCATCAA CGCCAGCGGC GTGGACGCCA AGGCCATCCT GTCTGCCAGA CTGAGCAAGA 2521 GCAGACGGCT GGAAAATCTG ATCGCCCAGC TGCCCGGCGG AAGTGGCAGC ATCAGCCTGA 2581 TCGCTGCTCT GGCAGTAGAC TACGTGATAG GAATGGAGAA TGCAATGCCC TGGAATCTGC 2641 CCGCAGACCT GGCTTGGTTT AAAAGAAACA CGCTTAATAA ACCAGTAATC ATGGGAAGAC 2701 ATACTTGGGA GAGTATAGGG CGGCCCCTGC CCGGCCGGAA AAATATTATA CTCTCAAGTC 2761 AACCGAGCAC TGACGATAGA GTCACCTGGG TGAAGTCCGT TGATGAGGCT ATAGCCGCAT 2821 GTGGAGATGT GCCTGAAATC ATGGTAATCG GTGGTGGAAG GGTCATAGAG CAATTCCTCC 2881 CGAAGGCTCA GAAACTTTAT CTTACACATA TAGACGCCGA GGTGGAGGGC GACACACATT 2941 TCCCTGACTA TGAACCCGAT GATTGGGAAA GCGTATTTAG TGAATTTCAC GACGCCGACG 3001 CACAGAACTC TCACAGTTAT TGCTTCGAGA TTCTGGAGCG ACGAGGCAGT GGAAGCGAGA 3061 AGAAGAATGG CCTGTTCGGA AACCTGATTG CCCTGAGCCT GGGCCTGACC CCCAACTTCA 3121 AGAGCAACTT CGACCTGGCC GAGGATGCCA AACTGCAGCT GAGCAAGGAC ACCTACGACG 3181 ACGACCTGGA CAACCTGCTG GCCCAGATCG GCGACCAGTA CGCCGACCTG TTTCTGGCCG 3241 CCAAGAACCT GTCCGACGCC ATCCTGCTGA GCGACATCCT GAGAGTGAAC ACCGAGATCA 3301 CCAAGGCCCC CCTGAGCGCC TCTATGATCA AGAGATACGA CGAGCACCAC CAGGACCTGA 3361 CCCTGCTGAA AGCTCTCGTG CGGCAGCAGC TGCCTGAGAA GTACAAAGAG ATTTTCTTCG 3421 ACCAGAGCAA GAACGGCTAC GCCGGCTACA TTGACGGCGG AGCCAGCCAG GAAGAGTTCT 3481 ACAAGTTCAT CAAGCCCATC CTGGAAAAGA TGGACGGCAC CGAGGAACTG CTCGTGAAGC 3541 TGAACAGAGA GGACCTGCTG CGGAAGCAGC GGACCTTCGA CAACGGCAGC ATCCCCCACC 3601 AGATCCACCT GGGAGAGCTG CACGCCATTC TGCGGCGGCA GGAAGATTTT TACCCATTCC 3661 TGAAGGACAA CCGGGAAAAG ATCGAGAAGA TCCTGACCTT CCGCATCCCC TACTACGTGG 3721 GCCCTCTGGC CAGGGGAAAC AGCAGATTCG CCTGGATGAC CAGAAAGAGC GAGGAAACCA 3781 TCACCCCCTG GAACTTCGAG GAAGTGGTGG ACAAGGGCGC TTCCGCCCAG AGCTTCATCG 3841 AGCGGATGAC CAACTTCGAT AAGAACCTGC CCAACGAGAA GGTGCTGCCC AAGCACAGCC 3901 TGCTGTACGA GTACTTCACC GTGTATAACG AGCTGACCAA AGTGAAATAC GTGACCGAGG 3961 GAATGAGAAA GCCCGCCTTC CTGAGCGGCG AGCAGAAAAA GGCCATCGTG GACCTGCTGT 4021 TCAAGACCAA CCGGAAAGTG ACCGTGAAGC AGCTGAAAGA GGACTACTTC AAGAAAATCG 4081 AGTGCTTCGA CTCCGTGGAA ATCTCCGGCG TGGAAGATCG GTTCAACGCC TCCCTGGGCA 4141 CATACCACGA TCTGCTGAAA ATTATCAAGG ACAAGGACTT CCTGGACAAT GAGGAAAACG 4201 AGGACATTCT GGAAGATATC GTGCTGACCC TGACACTGTT TGAGGACAGA GAGATGATCG 4261 AGGAACGGCT GAAAACCTAT GCCCACCTGT TCGACGACAA AGTGATGAAG CAGCTGAAGC 4321 GGCGGAGATA CACCGGCTGG GGCAGGCTGA GCCGGAAGCT GATCAACGGC ATCCGGGACA 4381 AGCAGTCCGG CAAGACAATC CTGGATTTCC TGAAGTCCGA CGGCTTCGCC AACAGAAACT 4441 TCATGCAGCT GATCCACGAC GACAGCCTGA CCTTTAAAGA GGACATCCAG AAAGCCCAGG 4501 TGTCCGGCCA GGGCGATAGC CTGCACGAGC ACATTGCCAA TCTGGCCGGC AGCCCCGCCA 4561 TTAAGAAGGG CATCCTGCAG ACAGTGAAGG TGGTGGACGA GCTCGTGAAA GTGATGGGCC 4621 GGCACAAGCC CGAGAACATC GTGATCGAAA TGGCCAGAGA GAACCAGACC ACCCAGAAGG 4681 GACAGAAGAA CAGCCGCGAG AGAATGAAGC GGATCGAAGA GGGCATCAAA GAGCTGGGCA 4741 GCCAGATCCT GAAAGAACAC CCCGTGGAAA ACACCCAGCT GCAGAACGAG AAGCTGTACC 4801 TGTACTACCT GCAGAATGGG CGGGATATGT ACGTGGACCA GGAACTGGAC ATCAACCGGC 4861 TGTCCGACTA CGATGTGGAC CATATCGTGC CTCAGAGCTT TCTGAAGGAC GACTCCATCG 4921 ACAACAAGGT GCTGACCAGA AGCGACAAGA ACCGGGGCAA GAGCGACAAC GTGCCCTCCG 4981 AAGAGGTCGT GAAGAAGATG AAGAACTACT GGCGGCAGCT GCTGAACGCC AAGCTGATTA 5041 CCCAGAGAAA GTTCGACAAT CTGACCAAGG CCGAGAGAGG CGGCCTGAGC GAACTGGATA 5101 AGGCCGGCTT CATCAAGAGA CAGCTGGTGG AAACCCGGCA GATCACAAAG CACGTGGCAC 5161 AGATCCTGGA CTCCCGGATG AACACTAAGT ACGACGAGAA TGACAAGCTG ATCCGGGAAG 5221 TGAAAGTGAT CACCCTGAAG TCCAAGCTGG TGTCCGATTT CCGGAAGGAT TTCCAGTTTT 5281 ACAAAGTGCG CGAGATCAAC AACTACCACC ACGCCCACGA CGCCTACCTG AACGCCGTCG 5341 TGGGAACCGC CCTGATCAAA AAGTACCCTA AGCTGGAAAG CGAGTTCGTG TACGGCGACT 5401 ACAAGGTGTA CGACGTGCGG AAGATGATCG CCAAGAGCGA GCAGGAAATC GGCAAGGCTA 5461 CCGCCAAGTA CTTCTTCTAC AGCAACATCA TGAACTTTTT CAAGACCGAG ATTACCCTGG 5521 CCAACGGCGA GATCCGGAAG CGGCCTCTGA TCGAGACAAA CGGCGAAACC GGGGAGATCG 5581 TGTGGGATAA GGGCCGGGAT TTTGCCACCG TGCGGAAAGT GCTGAGCATG CCCCAAGTGA 5641 ATATCGTGAA AAAGACCGAG GTGCAGACAG GCGGCTTCAG CAAAGAGTCT ATCCTGCCCA 5701 AGAGGAACAG CGATAAGCTG ATCGCCAGAA AGAAGGACTG GGACCCTAAG AAGTACGGCG 5761 GCTTCGACAG CCCCACCGTG GCCTATTCTG TGCTGGTGGT GGCCAAAGTG GAAAAGGGCA 5821 AGTCCAAGAA ACTGAAGAGT GTGAAAGAGC TGCTGGGGAT CACCATCATG GAAAGAAGCA 5881 GCTTCGAGAA GAATCCCATC GACTTTCTGG AAGCCAAGGG CTACAAAGAA GTGAAAAAGG 5941 ACCTGATCAT CAAGCTGCCT AAGTACTCCC TGTTCGAGCT GGAAAACGGC CGGAAGAGAA 6001 TGCTGGCCTC TGCCGGCGAA CTGCAGAAGG GAAACGAACT GGCCCTGCCC TCCAAATATG 6061 TGAACTTCCT GTACCTGGCC AGCCACTATG AGAAGCTGAA GGGCTCCCCC GAGGATAATG 6121 AGCAGAAACA GCTGTTTGTG GAACAGCACA AGCACTACCT GGACGAGATC ATCGAGCAGA 6181 TCAGCGAGTT CTCCAAGAGA GTGATCCTGG CCGACGCTAA TCTGGACAAA GTGCTGTCCG 6241 CCTACAACAA GCACCGGGAT AAGCCCATCA GAGAGCAGGC CGAGAATATC ATCCACCTGT 6301 TTACCCTGAC CAATCTGGGA GCCCCTGCCG CCTTCAAGTA CTTTGACACC ACCATCGACC 6361 GGAAGAGGTA CACCAGCACC AAAGAGGTGC TGGACGCCAC CCTGATCCAC CAGAGCATCA 6421 CCGGCCTGTA CGAGACACGG ATCGACCTGT CTCAGCTGGG AGGCGACAAA AGGCCGGCGG 6481 CCACGAAAAA GGCCGGCCAG GCAAAAAAGA AAAAGTAAga attcCTAGAG CTCGCTGATC 6541 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC 6601 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC 6661 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG 6721 GGAGGATTGG GAAGAgAATA GCAGGCATGC TGGGGAgcgg ccgcaggaac ccctagtgat 6781 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt 6841 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct 6901 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat 6961 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 7021 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 7081 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 7141 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt 7201 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 7261 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 7321 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 7381 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg 7441 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca 7501 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 7561 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 7621 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt 7681 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 7741 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 7801 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 7861 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 7921 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 7981 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 8041 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 8101 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 8161 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 8221 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 8281 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 8341 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 8401 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 8461 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 8521 ggagccggtg agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc 8581 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 8641 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 8701 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 8761 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 8821 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 8881 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 8941 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 9001 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 9061 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 9121 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 9181 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 9241 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 9301 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 9361 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 9421 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 9481 tgctggcctt ttgctcacat gt //