LOCUS       Exported                3416 bp ds-DNA     circular SYN 01-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 24, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     polyA_signal    26..74
                     /note="synthetic polyadenylation signal"
     misc_feature    88..179
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     protein_bind    192..245
                     /label=RE
                     /bound_moiety="p53"
                     /note="p53 response element created by fusing two human p53
                     binding sites"
     misc_feature    196..205
                     /label=p53
     misc_feature    226..235
                     /label=p53
     primer_bind     254..276
                     /label=SEQ MINIP FWD
     promoter        292..323
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(333..355)
                     /label=SEQ MiniP REV
     CDS             357..872
                     /codon_start=1
                     /label=NLuc_HS
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     polyA_signal    877..1101
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1208..1890)
                     /direction=LEFT
                     /label=ColE1 origin
     promoter        1929..2027
                     /label=cat Promoter
     CDS             2032..2844
                     /codon_start=1
                     /label=Kanamycin Resistance
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      2849..2956
     rep_origin      2961..3416
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 taacctcgag cgtctcagct tggagaataa aatatcttta ttttcattac atctgtgtgt
       61 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
      121 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctctc
      181 gctgtcagga gtacagaaca tgtctaagca tgctgtgcct tgcctggact tgcctggcct
      241 tgccttccct cgaggatatc aaatctggcc tcggcggcca agcttagaca ctagagggta
      301 tataatggaa gctcgacttc cagcttggca atccggtact gttggtaaag ccaccaatgg
      361 tgtttacctt ggaggacttc gtgggtgact ggaggcaaac tgcaggatac aatttggacc
      421 aggtcctcga acagggagga gtcagcagcc ttttccaaaa cttgggcgtg tccgttaccc
      481 ctattcagag aattgtcctt tccggagaga acggattgaa aatagatatc cacgtcataa
      541 tcccgtatga aggactgtct ggggaccaaa tgggtcagat tgaaaaaata ttcaaagtag
      601 tttatcccgt tgatgatcat cacttcaaag ttatccttca ttatgggact ttggtaatcg
      661 atggagttac tcctaatatg atagattact ttggccgacc ttacgagggg atcgctgtat
      721 tcgacggcaa gaaaatcacc gtcactggaa ccctctggaa cggcaacaag attattgacg
      781 agcgattgat caatccagac ggatctttgc ttttccgcgt aacgataaac ggcgtaacag
      841 ggtggaggct ctgcgaacga atactcgcct gagcttctgt gccttctagt tgccagccat
      901 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
      961 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     1021 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     1081 gggatgcggt gggctctatg gcgcttggct gagacgctcg agtgacacgg ttatccacag
     1141 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1201 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1261 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1681 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1741 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1801 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1861 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1921 ttttgtgatg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
     1981 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatgagccat
     2041 attcaacggg aaacgtcttg ctcaaggccg cgattaaatt ccaacatgga tgctgattta
     2101 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctaccgattg
     2161 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     2221 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     2281 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatcccaggg
     2341 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     2401 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     2461 gatcgcgtat ttcgactcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     2521 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     2581 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     2641 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     2701 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     2761 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     2821 catttgatgc tcgatgagtt tttctgattt tttttaaggc agttattggt gcccttaaac
     2881 gcctggttgc tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgatgat
     2941 aagctgtcaa acatgatgtt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3001 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3061 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3121 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3181 tgatggttca cgtagtgggc cagcgccctg atagacggtt tttcgccctt tgacgttgga
     3241 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3301 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3361 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caattt
//
