LOCUS       Exported               13636 bp ds-DNA     circular SYN 24-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 24, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     polyA_signal    14..238
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(243..1922)
                     /codon_start=1
                     /label=GrLuc
                     /translation="MKREKNVVYGPEPKHPLGNFTAGEMLYNALHKHSHIPQAILDVMG
                     NESLSYQEFFDTTVKLGQSLQNCGYKMNDVVSICAENNKRFFIPIISAWYIGMVVAPVN
                     EDYIPDELCKVTGISKPILVFTTRKILPKVLEVKDRTNYIKRIIILDSEENLLGCESLH
                     NFMSRYSDNNLQTFKPLHYDPVDQVAAILCSSGTTGLPKGVMQTHRNICVRLTHASDPR
                     VGTQLIPGVSVLAYLPFFHAFGFSINLGYFMVGLRVVMLRRFNQEVFLKAIQDYEVRSV
                     INVPSTILFLSKSPLVDKYDLSTLAELCCGAAPLAKEVAEIAVKRLNLPGIRCGYGLTE
                     STSANIHTLHNEFKSGSLGKVTPYMAAKIIDRNTGEALGPNQVGELCIWGPMVTKGYVN
                     NPQATKEAIDDDGWLHSGDFGYYDEDEYFYIVDRYKELIKYKGYQVAPVELEEILLQHP
                     GIRDVAVVGIPDIEAGELPAGFVVKQPGAQLTAKEVYDFLAQRVSHSKYLRGGVRFVDS
                     IPRNVTGKISRKELREALMEKAGGGSMYKTSAANDENYALAA"
     primer_bind     243..264
                     /label=ASP16JUN08
     primer_bind     complement(1901..1922)
                     /label=ASP16JUN07
     primer_bind     1923..1946
                     /label=ASP16AUG06
     primer_bind     1927..1948
                     /label=ASP16MAY02
     promoter        complement(1955..2158)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2159..2462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(2498..2526)
                     /label=ASP16MAY01
     polyA_signal    2540..2588
                     /note="synthetic polyadenylation signal"
     misc_feature    2602..2693
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     protein_bind    2706..2759
                     /label=RE
                     /bound_moiety="NF-kappa-B"
                     /note="NF-kappa-B response element (5 copies)"
     misc_feature    2706..2713
                     /label=NkB RE
     misc_feature    2717..2724
                     /label=NkB RE
     misc_feature    2728..2735
                     /label=NkB RE
     misc_feature    2736..2739
                     /label=ASP17JANA2
     misc_feature    2740..2747
                     /label=NkB RE
     misc_feature    2750..2757
                     /label=NkB RE
     primer_bind     2768..2790
                     /label=SEQ MINIP FWD
     promoter        2806..2837
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(2847..2869)
                     /label=SEQ MiniP REV
     CDS             2871..4517
                     /codon_start=1
                     /label=RedFirefly
                     /translation="MENMENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFT
                     NAVTGVDYSYAEYLEKSCCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGV
                     APTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGY
                     QCLDTFIKRNTPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFS
                     HARDPIYGNQVSPGTAVLTVVPLHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQD
                     YKCTYVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQ
                     GYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPML
                     MKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELES
                     VLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGG
                     VRFVDEVPKGLTGKIDGRAIREILKKPVAKM"
     polyA_signal    4522..4746
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4759..4807
                     /note="synthetic polyadenylation signal"
     misc_feature    4821..4912
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_binding    4925..4932
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    4937..4944
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    4955..4962
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    4969..4976
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    4984..4991
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    4999..5006
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    5013..5020
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     primer_bind     5035..5057
                     /label=SEQ MINIP FWD
     promoter        5073..5104
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(5114..5136)
                     /label=SEQ MiniP REV
     primer_bind     5137..5161
                     /label=ASP16MAY03
     gene            5138..6790
                     /gene="luc"
                     /label=luc
                     /note="LUC"
     CDS             5138..6790
                     /codon_start=1
                     /transl_table=11
                     /gene="luc"
                     /product="Firelfly luciferase Photinus pyralis"
                     /label=Firefly Luciferase
                     /note="firefly luciferase"
                     /db_xref="GI:155733599"
                     /protein_id="ABU39926.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(6771..6791)
                     /label=ASP16MAY04
     polyA_signal    6798..7022
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    7035..7083
                     /note="synthetic polyadenylation signal"
     misc_feature    7097..7188
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_binding    7199..7224
                     /label=DBE
                     /bound_moiety="FOXO"
     misc_binding    7231..7256
                     /label=DBE
                     /bound_moiety="FOXO"
     misc_binding    7264..7289
                     /label=DBE
                     /bound_moiety="FOXO"
     primer_bind     7298..7320
                     /label=SEQ MINIP FWD
     promoter        7336..7367
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(7377..7399)
                     /label=SEQ MiniP REV
     CDS             7401..8333
                     /codon_start=1
                     /label=Renilla_HS
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    8341..8565
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    8578..8626
                     /note="synthetic polyadenylation signal"
     misc_feature    8640..8731
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     protein_bind    8744..8797
                     /label=RE
                     /bound_moiety="p53"
                     /note="p53 response element created by fusing two human p53
                     binding sites"
     misc_feature    8748..8757
                     /label=p53
     misc_feature    8778..8787
                     /label=p53
     primer_bind     8806..8828
                     /label=SEQ MINIP FWD
     promoter        8844..8875
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(8885..8907)
                     /label=SEQ MiniP REV
     CDS             8909..9424
                     /codon_start=1
                     /label=NLuc_HS
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     polyA_signal    9429..9653
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    9666..9714
                     /note="synthetic polyadenylation signal"
     misc_feature    9728..9819
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_binding    9832..9838
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    9846..9852
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    9856..9862
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    9865..9871
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    9874..9880
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    9882..9888
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     primer_bind     9904..9926
                     /label=SEQ MINIP FWD
     promoter        9942..9973
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(9983..10005)
                     /label=SEQ MiniP REV
     primer_bind     10006..10029
                     /label=ASP16JUL18
     CDS             10007..10942
                     /codon_start=1
                     /label=GreenRenilla_HS
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNATSSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGSALAFHYAYEHQDRIKAIVHMESVVDVIESWMGWPDIEEEL
                     ALIKSEEGEKMVLENNFFVETLLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKLFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFLQEDAPDEMGKYIKSFVERVLKNEQ"
     primer_bind     10007..10031
                     /label=ASP16JUN01
     primer_bind     complement(10670..10694)
                     /label=ASP16JUL19
     primer_bind     10694..10715
                     /label=ASP16JUL20
     primer_bind     complement(10924..10947)
                     /label=ASP16JUN02
     polyA_signal    10947..11171
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     11176..11199
                     /label=ASP14NOV50
     rep_origin      complement(11261..11943)
                     /direction=LEFT
                     /label=ColE1 origin
     primer_bind     complement(11956..11981)
                     /label=ASP14NOV51
     primer_bind     11978..12000
                     /label=ASP14OCT46
     primer_bind     11978..11999
                     /label=ASP16AUG01
     promoter        11982..12115
                     /label=Spm promoter
     CDS             12116..13126
                     /codon_start=1
                     /gene="SpnR"
                     /label=SpmR
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      13127..13176
     primer_bind     complement(13154..13180)
                     /label=ASP16AUG02
     primer_bind     complement(13154..13177)
                     /label=ASP14OCT47
     primer_bind     13177..13200
                     /label=ASP15JAN01
     rep_origin      13181..13636
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(join(13605..13636,1..5))
                     /label=ASP15JUL28
ORIGIN
        1 taacggatca gcgccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
       61 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
      121 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
      181 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagaa
      241 gctcaagccg ccagggcata gttctcatcg ttggcggcgg atgtcttgta catagatcca
      301 ccacccgcct tctccataag tgcttctcgg agttcctttc ggctgatttt gcccgtgacg
      361 ttcctcggaa tagagtcaac gaatctgaca ccccctcgaa gatacttgct gtggctaact
      421 ctttgtgcaa ggaaatcata tacttcttta gccgtcagtt gggccccagg ttgcttgaca
      481 acgaatccgg ccggaagttc ccctgcttct atgtcgggga taccgactac ggccacatcg
      541 cggatccctg gatgttggag caaaatctcc tccaactcga caggagccac ttgatatccc
      601 ttgtacttga tcagctcttt atatctatcc acaatgtaaa aatattcatc ctcatcatag
      661 tagccaaaat caccactgtg aagccaacca tcgtcgtcaa tggcctcctt ggtggcctgt
      721 ggattattca cgtagccttt cgtgaccatg gggccccaaa tgcacaactc tcccacttga
      781 tttgggccaa gcgcctctcc ggtgtttcga tctataattt tagcggccat gtagggcgtc
      841 acttttccca atgaaccgga tttaaattcg ttgtgaagag tgtggatatt agcggatgtg
      901 ctttcagtca acccgtaacc acaccgtatg cctggcaaat taaggcgttt tacggcgatt
      961 tcagctactt cttttgcaag aggtgccgca ccacaacaaa gttccgccaa ggtagaaaga
     1021 tcatacttat caaccaacgg tgatttagaa agaaagagga ttgtacttgg gacattgatc
     1081 acacttcgta cttcatagtc ttgaatcgcc ttcaaaaaaa cctcttggtt gaacctccga
     1141 agcatcacga cgcgcagccc caccataaaa taccccaagt taatggaaaa cccaaaggcg
     1201 tgaaagaagg gaagatacgc cagcacacta actccgggaa tgagttgggt accaactctc
     1261 gggtcagagg cgtgggtcag ccgaacacat atattgcggt gggtctgcat gacgccctta
     1321 ggcaacccgg tagtaccact actacacagt atcgctgcaa cctggtcgac cggatcgtaa
     1381 tgcagtggtt taaaggtctg aagattgtta tcgctatatc ttgacataaa attatgcaag
     1441 gattcacatc cgagaaggtt ttcctcagag tccagaatta tgattctttt gatataattt
     1501 gttctatcct taacctccaa aacctttgga agtatctttc gcgtggtgaa cacaagtatt
     1561 ggcttactaa ttcctgtgac cttacacagt tcgtctggta tatagtcctc gtttacaggt
     1621 gcaacgacca tgcctatata ccacgcacta ataattggaa tgaaaaatct tttgttgttt
     1681 tcagcgcata ttgatacaac atcattcatt ttatagccac aattctggag actctgcccc
     1741 aatttgaccg tcgtgtcgaa gaattcctga taactaagac tttcgttacc catcacgtcg
     1801 aggatcgcct gcgggatgtg actatgtttg tgaagcgcgt tgtagagcat ctctccagcg
     1861 gtaaaattcc caagcgggtg cttaggctcg ggcccataca caacattctt ctctctcttc
     1921 atcatttgag cttgatctga cggttcacta aaccagctct gcttatatag acctcccacc
     1981 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     2041 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     2101 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     2161 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     2221 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     2281 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     2341 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     2401 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     2461 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     2521 tactccggat cccagggaga ataaaatatc tttattttca ttacatctgt gtgttggttt
     2581 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
     2641 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctcgcatgc
     2701 atgaggggaa tttccgggga ctttcccggg aatttgcggg ggactttccg ggaatttcct
     2761 ccctcgagga tatcaaatct ggcctcggcg gccaagctta gacactagag ggtatataat
     2821 ggaagctcga cttccagctt ggcaatccgg tactgttggt aaagccacca atggagaaca
     2881 tggagaacga cgagaacatt gtggtgggac ccaagccgtt ctacccgatc gaggagggtt
     2941 ccgctggcac ccagctgcgc aagtacatgg agcgctacgc taagctgggt gctatcgcct
     3001 tcaccaacgc cgtgaccggt gtggattact cctacgccga gtacctggag aagtcctgct
     3061 gcctcggaaa ggccctgcag aactacggtc tggtcgtgga tggccgcatt gccctctgct
     3121 cggagaactg cgaggagttc ttcattcccg tgatcgctgg tctgttcatc ggagtcggcg
     3181 ttgccccaac caacgagatc tacaccctgc gcgagctggt ccacagcctg ggtatcagca
     3241 agccgaccat cgttttctcg agcaagaagg gcctcgacaa ggttatcacc gtgcaaaaaa
     3301 ccgtcaccac catcaagacc atcgttatcc tggattccaa ggttgactac cgcggttacc
     3361 agtgcctcga caccttcatc aagcgtaaca ccccacccgg cttccaagcc tcctccttca
     3421 aaaccgtgga ggttgatcgc aaggagcagg tggccctgat catgaactcc tccggctcca
     3481 ccggtctgcc aaagggcgtg cagctgaccc acgagaacac cgtcacccgc ttctcgcatg
     3541 cccgtgatcc catctacggc aaccaggtgt ccccaggcac cgccgtcctg accgtcgtgc
     3601 ccctccacca cggcttcggc atgttcacca ctctcggcta cttgatctgc ggcttccgtg
     3661 tggtgatgct gactaagttc gacgaggaga ctttcctcaa gaccctgcaa gactacaagt
     3721 gcacttacgt gatcctggtg ccgaccctgt tcgccatcct gaacaagagc gagctgttga
     3781 acaaatacga cctgtcgaac ctggtggaga tcgcttccgg cggcgcccca ctgtcgaagg
     3841 aagttggcga ggccgttgct cgccgcttca acctgcccgg cgtccgtcag ggttacggcc
     3901 tgaccgagac tacctcggct atcatcatca cccccgaggg cgacgataag cccggcgctt
     3961 ccggcaaggt tgttccactc ttcaaagcca aggtgatcga cctggacacc aagaagtccc
     4021 tgggtcccaa ccgtcgcgga gaagtgtgcg tcaagggccc catgctgatg aagggctacg
     4081 tcaacaaccc agaggccacc aaggagttga tcgacgagga gggctggctg cacactggtg
     4141 acatcggtta ctacgacgaa gagaagcact tcttcatcgt tgaccgcctg aagtccttga
     4201 tcaagtacaa gggctaccag gttccccccg ccgagctcga gagcgtcctg ctgcagcacc
     4261 cctccatttt cgatgccgga gtggccggtg tccccgatcc cgtggctggc gagctgccag
     4321 gtgccgtcgt cgtgctggag tccggcaaga acatgaccga gaaggaagtg atggattacg
     4381 tggcctcgca agtgtccaac gccaagcgcc tgcgcggcgg cgtgcgcttc gtggatgagg
     4441 tgcccaaggg cctcaccgga aagatcgacg gtcgcgctat ccgcgagatc ctcaagaagc
     4501 ccgtggccaa gatgtgagct tctgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4561 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4621 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4681 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4741 ctatggcgct gtcaggagaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     4801 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa
     4861 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctcgctgtca
     4921 ggaggtctag actgccgtct agacttaaca gtacgtctag actcggccgt ctagacttag
     4981 tacgtctaga ctcggtatgt ctagactgac ccgtctagac ggatcctccc tcgaggatat
     5041 caaatctggc ctcggcggcc aagcttagac actagagggt atataatgga agctcgactt
     5101 ccagcttggc aatccggtac tgttggtaaa gccaccaatg gaagacgcca aaaacataaa
     5161 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
     5221 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
     5281 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
     5341 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     5401 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
     5461 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
     5521 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
     5581 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
     5641 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
     5701 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
     5761 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
     5821 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     5881 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     5941 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
     6001 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     6061 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
     6121 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     6181 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     6241 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     6301 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     6361 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     6421 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     6481 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     6541 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     6601 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     6661 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     6721 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     6781 cgccgtgtaa tgagcttctg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6841 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6901 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6961 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     7021 ggcgctaatg ggagaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     7081 gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag
     7141 caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctcg ctgaattcga
     7201 tcaagtaaac aactatgtaa acaagatgca gatcaagtaa acaactatgt aaacaactcc
     7261 gttgatcaag taaacaacta tgtaaacaat ccctcgagga tatcaaatct ggcctcggcg
     7321 gccaagctta gacactagag ggtatataat ggaagctcga cttccagctt ggcaatccgg
     7381 tactgttggt aaagccacca atgacttcca aggtttatga ccccgagcaa cgcaagagaa
     7441 tgataactgg gcctcagtgg tgggccagat gtaaacaaat gaatgtcctt gatagtttca
     7501 tcaattacta cgacagtgaa aagcatgcag aaaacgccgt aatttttttg catggcaacg
     7561 cagcctcctc ttatttgtgg cgacacgtag tcccgcatat agagccggta gctaggtgta
     7621 ttatccctga tctgatcgga atgggaaaga gtggcaagtc agggaatgga tcttatcgcc
     7681 ttcttgacca ctacaagtat ctgacggctt ggttcgaact cttgaatttg ccgaagaaaa
     7741 tcatcttcgt gggtcacgat tggggagcct gtctcgcgtt ccattattcc tacgagcatc
     7801 aggacaagat aaaggcaatc gttcacgccg agtctgtagt agatgtaatc gagtcctggg
     7861 acgaatggcc agatattgag gaggatatag cattgattaa aagcgaggaa ggggaaaaga
     7921 tggtgctgga gaacaacttc tttgtggaaa caatgcttcc ttcaaaaatc atgagaaaac
     7981 tggaacccga ggaatttgcc gcttatctcg aaccgtttaa agaaaaggga gaagtccgca
     8041 ggccaacact ttcctggcca agagaaatcc cgctcgtcaa aggcggaaag ccggatgttg
     8101 tgcaaatagt ccgaaattac aacgcgtacc tccgggcctc tgacgatctc cctaaaatgt
     8161 ttatagaatc agatccagga ttttttagca acgctatcgt agaaggagca aagaaatttc
     8221 caaataccga atttgtcaaa gtaaagggac tgcatttcag tcaagaggac gctccagacg
     8281 agatgggcaa atacataaag tcctttgtag agagggtcct gaagaacgaa cagtgagctt
     8341 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     8401 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     8461 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     8521 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcgctg cttggagaat
     8581 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     8641 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     8701 agtgcaagtg caggtgccag aacatttctc tcgctgtcag gagtacagaa catgtctaag
     8761 catgctgtgc cttgcctgga cttgcctggc cttgccttcc ctcgaggata tcaaatctgg
     8821 cctcggcggc caagcttaga cactagaggg tatataatgg aagctcgact tccagcttgg
     8881 caatccggta ctgttggtaa agccaccaat ggtgtttacc ttggaggact tcgtgggtga
     8941 ctggaggcaa actgcaggat acaatttgga ccaggtcctc gaacagggag gagtcagcag
     9001 ccttttccaa aacttgggcg tgtccgttac ccctattcag agaattgtcc tttccggaga
     9061 gaacggattg aaaatagata tccacgtcat aatcccgtat gaaggactgt ctggggacca
     9121 aatgggtcag attgaaaaaa tattcaaagt agtttatccc gttgatgatc atcacttcaa
     9181 agttatcctt cattatggga ctttggtaat cgatggagtt actcctaata tgatagatta
     9241 ctttggccga ccttacgagg ggatcgctgt attcgacggc aagaaaatca ccgtcactgg
     9301 aaccctctgg aacggcaaca agattattga cgagcgattg atcaatccag acggatcttt
     9361 gcttttccgc gtaacgataa acggcgtaac agggtggagg ctctgcgaac gaatactcgc
     9421 ctgagcttct gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     9481 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     9541 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     9601 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcgcttgg
     9661 cggagaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     9721 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
     9781 ctgtccccag tgcaagtgca ggtgccagaa catttctctc gctgcagact atgagtcaaa
     9841 cagcgtgact caagttgagt cagttgactc acttgagtca ctgactcagg catgctccct
     9901 cgaggatatc aaatctggcc tcggcggcca agcttagaca ctagagggta tataatggaa
     9961 gctcgacttc cagcttggca atccggtact gttggtaaag ccaccaatgg cttccaaagt
    10021 ctacgaccca gaacaaagga agaggatgat tactggaccg cagtggtggg caaggtgcaa
    10081 gcagatgaat gtactcgata gttttatcaa ttattacgac tccgaaaaac acgcggaaaa
    10141 tgctgtcata ttcctgcacg ggaacgccac tagcagctac ttgtggcggc acgtagtccc
    10201 ccacatagag cctgttgccc gatgtattat tcccgatctc attggaatgg gcaaatcagg
    10261 caaatctggg aacggttctt accgcctgct cgatcactat aaatatttga ccgcttggtt
    10321 tgagctgctt aatctcccta aaaaaataat attcgttgga cacgactggg gctcagctct
    10381 ggcgttccac tacgcatacg agcatcaaga ccgaataaaa gcaatagtac atatggagtc
    10441 agttgttgac gtcatagagt catggatggg ttggccagat attgaagagg aattggccct
    10501 catcaagagt gaggaggggg aaaaaatggt cctggaaaat aattttttcg tggaaacctt
    10561 gctcccgagt aaaataatgc gaaaacttga acccgaagag tttgctgctt atttggagcc
    10621 tttcaaggaa aagggggaag taagacgacc aaccctttct tggccgaggg agatacctct
    10681 tgttaagggt ggaaagcccg acgtagtcca gatagtccga aattacaacg cgtacctgag
    10741 agctagtgat gacctcccta agttgtttat cgagtcagac cctgggttct tcagcaacgc
    10801 aattgtagaa ggagcgaaaa aatttccaaa tacagaattt gtgaaggtta aggggctcca
    10861 ctttttgcaa gaggacgccc ccgatgagat gggaaaatac attaaatctt tcgtagagcg
    10921 cgtccttaag aatgaacagt gagcttctgt gccttctagt tgccagccat ctgttgtttg
    10981 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
    11041 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
    11101 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
    11161 gggctctatg gcgcttgaca cggttatcca cagaatcagg ggataacgca ggaaagaaca
    11221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    11281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    11341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    11401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    11461 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    11521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    11581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    11641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    11701 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    11761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    11821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    11881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    11941 tgagattatc aaaaaggatc ttcacctaga tccttttgtg accagccagg acagaaatgc
    12001 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
    12061 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
    12121 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
    12181 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
    12241 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
    12301 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
    12361 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
    12421 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
    12481 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
    12541 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
    12601 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
    12661 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
    12721 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
    12781 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
    12841 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
    12901 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtagctgc
    12961 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
    13021 ggcttatctt ggacaagaag aagatcgttt ggcctcgcgc gcagatcagt tggaagaatt
    13081 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatcac tagaccaatg
    13141 ttacacatat atactttaga ttgatttaaa acttcatgtt acgcgccctg tagcggcgca
    13201 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    13261 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    13321 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    13381 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc cagcgccctg atagacggtt
    13441 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    13501 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    13561 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    13621 ttaacgctta caattt
//
