LOCUS Exported 3386 bp ds-DNA circular SYN 07-FEB-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 3386) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Feb 7, 2019 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3386 /organism="recombinant plasmid" /mol_type="other DNA" CDS complement(468..1328) /codon_start=1 /transl_table=11 /product="ampicillin resistance protein" /label=ampicillin resistance protein /db_xref="GI:672234590" /protein_id="AIJ03087.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(1329..1433) /gene="bla" /label=AmpR promoter rep_origin 1460..1915 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" polyA_signal 2046..2094 /note="synthetic polyadenylation signal" misc_feature 2108..2199 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" misc_feature 2224..2574 /label=8x gRNA1 binding sites /note="8x gRNA1 binding sites" misc_feature 2253..2255 /label=PAM misc_feature 2256..2274 /label=target misc_feature 2297..2315 /label=target misc_feature 2338..2356 /label=target misc_feature 2379..2388 /label=fract_5p misc_feature 2389..2396 /label=frac_3P misc_feature 2419..2437 /label=target misc_feature 2460..2478 /label=target misc_feature 2501..2519 /label=target misc_feature 2542..2560 /label=target promoter 2581..2614 /label=Minimal Ade MLP /note="Minimal Ade MLP" misc_feature 2642..2671 /label=BpiI placeholder polyA_signal 2688..2909 /gene="SV40" gene 2688..2909 /gene="SV40" /label=SV40 polyA_signal 2697..2818 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(join(3095..3386,1..297)) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 61 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 121 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 181 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 241 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 301 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 361 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 421 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 481 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 541 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 601 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 661 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 721 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 781 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 841 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 901 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 961 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 1021 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 1081 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 1141 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 1201 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 1261 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 1321 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 1381 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 1441 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 1501 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 1561 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 1621 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 1681 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 1741 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 1801 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 1861 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccat 1921 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 1981 cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg 2041 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga 2101 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg 2161 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgatagcta gctgaaatat 2221 taatgtcgcg actattaata ttttcaggct agccatgctt cgctacacgc gactattaat 2281 attttcaggc tagccatgct tcgctacacg cgactattaa tattttcaag ctagccatgc 2341 ttcgctacac gcgactatta atattttcag gctagccatg cttcgctaac gcgactatta 2401 atattttcag gctagtcatg cttcgctaca cgcgactatt aatattttca ggctagccat 2461 gcttcgctac acgcgactat taatattttc aggctagcca tgcttcgcta cacgcgacta 2521 ttaatatttt caggctagcc atgcttcgct acacgcgact attaatattt tcaggctagc 2581 ggggggctat aaaagggggt gggggcgttc gtcctgctat ctagcgtcgc gttgaccatg 2641 ccaacatgtc ttcgaattcg aagactagga cggccggccg cttcgagcag acatgataag 2701 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg 2761 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa 2821 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta 2881 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatcgagtat gtcgaccgat 2941 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 3001 cgccgcactt atgatgcaac tcgtaggaca ggtgcatgtg agcaaaaggc cagcaaaagg 3061 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 3121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 3181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 3241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct 3301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 3361 ccgttcagcc cgaccgctgc gcctta //