LOCUS       pCR8_12s.ape            3468 bp ds-DNA     linear       08-MAR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    683..1333
                     /label="TaNAM-A1(1-217)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        537..553
                     /label="M13_forward20_primer"
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_fwdcolor=#346ee0
     misc_feature    522..544
                     /label="M13_pUC_fwd_primer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(1483..1501)
                     /label="M13_reverse_primer"
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_fwdcolor=#346ee0
     source          1334..3468
                     /label="source:pCR8/GW/TOPO"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(569..668)
                     /label="attL1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            1715..2725
                     /label="spect"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      2802..3421
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1715..2725
                     /label="ORF frame 2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      complement(268..295)
                     /label="rrnB_T2_terminator"
                     /ApEinfo_revcolor=#9d1b1c
                     /ApEinfo_fwdcolor=#9d1b1c
     terminator      complement(427..470)
                     /label="rrnB_T1_terminator"
                     /ApEinfo_revcolor=#9d1b1c
                     /ApEinfo_fwdcolor=#9d1b1c
     source          1..682
                     /label="source:pCR8/GW/TOPO"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1347..1446
                     /label="attL2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTCC GAATTCGCCC TTATGAGGTC CATGGGCAGC TCCGACTCAT CCTCCGGCTC
      721 GGCGCAAAAA GCAGCGCGGC ATCAGCATGA GCCGCCGCCT CCGCGGCAGC GGGGCTCGGC
      781 GCCGGAGCTC CCACCGGGCT TCCGGTTCCA CCCGACGGAC GAGGAGCTGG TCGTGCACTA
      841 CCTCAAGAAG AAGGCCGCCA AGGTGCCGCT CCCCGTCACC ATCATCGCCG AGGTGGATCT
      901 CTACAAGTTC GACCCATGGG AGCTCCCCGA GAAGGCGACC TTCGGGGAGC AGGAGTGGTA
      961 CTTCTTCAGC CCGCGCGACC GCAAGTACCC CAACGGCGCG CGGCCGAACC GGGCGGCGAC
     1021 GTCGGGCTAC TGGAAGGCCA CCGGCACGGA CAAACCTATC CTGGCCTCGG GGACGGGGTG
     1081 CGGCCTGGTC CGGGAGAAGC TCGGCGTCAA GAAGGCGCTC GTCTTCTACC GCGGGAAGCC
     1141 GCCCAAGGGC CTCAAAACCA ACTGGATCAT GCACGAGTAC CGCCTCACCG ACGCGTCTGG
     1201 CTCCACCACC ACCAGCCGGC CGCCGCCGCC TGTGACCGGC GGGAGCCGGG CTGCAGCCTC
     1261 TCTGAGGTTG GACGACTGGG TGCTGTGCCG CATCTACAAG AAGATCAACA AGGCCGCGGC
     1321 CGGAGATCAG CAGAAGGGCG AATTCGACCC AGCTTTCTTG TACAAAGTTG GCATTATAAA
     1381 AAATAATTGC TCATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT
     1441 TATTTGCCAT CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC
     1501 CTGGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAAATAT
     1561 ATCATCATGC CTCCTCTAGA CCAGCCAGGA CAGAAATGCC TCGACTTCGC TGCTGCCCAA
     1621 GGTTGCCGGG TGACGCACAC CGTGGAAACG GATGAAGGCA CGAACCCAGT GGACATAAGC
     1681 CTGTTCGGTT CGTAAGCTGT AATGCAAGTA GCGTATGCGC TCACGCAACT GGTCCAGAAC
     1741 CTTGACCGAA CGCAGCGGTG GTAACGGCGC AGTGGCGGTT TTCATGGCTT GTTATGACTG
     1801 TTTTTTTGGG GTACAGTCTA TGCCTCGGGC ATCCAAGCAG CAAGCGCGTT ACGCCGTGGG
     1861 TCGATGTTTG ATGTTATGGA GCAGCAACGA TGTTACGCAG CAGGGCAGTC GCCCTAAAAC
     1921 AAAGTTAAAC ATCATGAGGG AAGCGGTGAT CGCCGAAGTA TCGACTCAAC TATCAGAGGT
     1981 AGTTGGCGTC ATCGAGCGCC ATCTCGAACC GACGTTGCTG GCCGTACATT TGTACGGCTC
     2041 CGCAGTGGAT GGCGGCCTGA AGCCACACAG TGATATTGAT TTGCTGGTTA CGGTGACCGT
     2101 AAGGCTTGAT GAAACAACGC GGCGAGCTTT GATCAACGAC CTTTTGGAAA CTTCGGCTTC
     2161 CCCTGGAGAG AGCGAGATTC TCCGCGCTGT AGAAGTCACC ATTGTTGTGC ACGACGACAT
     2221 CATTCCGTGG CGTTATCCAG CTAAGCGCGA ACTGCAATTT GGAGAATGGC AGCGCAATGA
     2281 CATTCTTGCA GGTATCTTCG AGCCAGCCAC GATCGACATT GATCTGGCTA TCTTGCTGAC
     2341 AAAAGCAAGA GAACATAGCG TTGCCTTGGT AGGTCCAGCG GCGGAGGAAC TCTTTGATCC
     2401 GGTTCCTGAA CAGGATCTAT TTGAGGCGCT AAATGAAACC TTAACGCTAT GGAACTCGCC
     2461 GCCCGACTGG GCTGGCGATG AGCGAAATGT AGTGCTTACG TTGTCCCGCA TTTGGTACAG
     2521 CGCAGTAACC GGCAAAATCG CGCCGAAGGA TGTCGCTGCC GACTGGGCAA TGGAGCGCCT
     2581 GCCGGCCCAG TATCAGCCCG TCATACTTGA AGCTAGACAG GCTTATCTTG GACAAGAAGA
     2641 AGATCGCTTG GCCTCGCGCG CAGATCAGTT GGAAGAATTT GTCCACTACG TGAAAGGCGA
     2701 GATCACCAAG GTAGTCGGCA AATAACCCTC GAGCCACCCA TGACCAAAAT CCCTTAACGT
     2761 GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG
     2821 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC
     2881 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG
     2941 CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA
     3001 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC
     3061 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC
     3121 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA
     3181 CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG
     3241 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT
     3301 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA
     3361 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC
     3421 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTT
//