LOCUS       Exported                9201 bp ds-DNA     circular SYN 21-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 8, 2019 from SnapGene Viewer 4.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9201
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    65..583
                     /gene="CEN6_ARS4"
                     /label=CEN6_ARS4
     gene            715..1575
                     /gene="Ampicillin"
                     /product="ampicillin resistance gene"
                     /label=Ampicillin
     rep_origin      1730..2349
                     /gene="pBR322_origin"
                     /label=pBR322_origin
     misc_feature    2828..2861
                     /gene="loxP"
                     /label=loxP
     promoter        2871..3259
                     /gene="pAgTEF"
     gene            3260..4069
                     /gene="kanMX"
                     /product="geneticin resistance protein"
                     /label=kanMX
     misc_feature    4074..4309
                     /gene="tAgTEF"
                     /label=tAgTEF
     misc_feature    4335..4368
                     /gene="loxP"
                     /label=loxP
     misc_feature    4401..4809
                     /gene="TEFmut6"
                     /product="TEF1 promoter mutant 6"
                     /label=TEFmut6
     gene            4810..5526
                     /gene="yeCitrine"
                     /product="Citrine"
                     /label=yeCitrine
     terminator      5527..5782
                     /gene="tCYC1"
     terminator      complement(5797..5961)
                     /gene="TADH1"
                     /label=TADH1 terminator
                     /label=TADH1
     misc_feature    complement(5984..6694)
                     /label=mCherry
     misc_feature    6083..6104
                     /label=mCherry-mCherry F
     misc_feature    complement(6695..7104)
                     /label=TEF1 promoter mutant 8
     gene            7982..8785
                     /gene="URA3"
                     /product="URA3 marker"
                     /label=URA3
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctgcag ctgaagcttc gtacgctgca ggtcgacaac
     2821 ccttaatata acttcgtata atgtatgcta tacgaagtta ttaggtctag agatctgttt
     2881 agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc
     2941 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc
     3001 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa
     3061 gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg
     3121 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac
     3181 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca
     3241 catccgaaca taaacaacca tgggtaagga aaagactcac gtttcgaggc cgcgattaaa
     3301 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     3361 aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     3421 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     3481 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     3541 actcaccact gcgatccccg gcaaaacagc attccaggta ttagaagaat atcctgattc
     3601 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     3661 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     3721 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     3781 acaagtctgg aaagaaatgc ataagctttt gccattctca ccggattcag tcgtcactca
     3841 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3901 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3961 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     4021 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagtactgac
     4081 aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt
     4141 gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc
     4201 ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg
     4261 gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtgtcgaa aacgagctct
     4321 cgagaaccct taatataact tcgtataatg tatgctatac gaagttatta ggtgatatca
     4381 gatccactag tggcctatgc atagcttcag aatgtttcta ctcctttttt actcttccag
     4441 attttctcgg actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa
     4501 atttcccctc tttcttcctc tagggtgtcg ttaactaccc gtactaaagg tttggaaaaa
     4561 aaaaagagac cgcctcgttt ctttttcttc gccgaaaaag gcaataaaaa tttttatcac
     4621 gtttcttttt cttgaaaatt ttttttttga tttttttctc tttcgatgac ctcccattga
     4681 tatttaagtt aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgctcta
     4741 ttacaacttt ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt
     4801 tctagaaaaa tgtctaaagg tgaagaatta ttcactggtg ttgtcccaat tttggttgaa
     4861 ttagatggtg atgttaatgg tcacaaattt tctgtctccg gtgaaggtga aggtgatgct
     4921 acttacggta aattgacctt aaaatttatt tgtactactg gtaaattgcc agttccatgg
     4981 ccaaccttag tcactacttt aggttatggt ttgatgtgtt ttgctagata cccagatcat
     5041 atgaaacaac atgacttttt caagtctgcc atgccagaag gttatgttca agaaagaact
     5101 atttttttca aagatgacgg taactacaag accagagctg aagtcaagtt tgaaggtgat
     5161 accttagtta atagaatcga attaaaaggt attgatttta aagaagatgg taacatttta
     5221 ggtcacaaat tggaatacaa ctataactct cacaatgttt acatcatggc tgacaaacaa
     5281 aagaatggta tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg ttctgttcaa
     5341 ttagctgacc attatcaaca aaatactcca attggtgatg gtccagtctt gttaccagac
     5401 aaccattact tatcctatca atctgcctta tccaaagatc caaacgaaaa gagagaccac
     5461 atggtcttgt tagaatttgt tactgctgct ggtattaccc atggtatgga tgaattgtac
     5521 aaataaatcg ataccgtcga cctcgagtca tgtaattagt tatgtcacgc ttacattcac
     5581 gccctcctcc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
     5641 tccctattta ttttttttaa tagttatgtt agtattaaga acgttattta tatttcaaat
     5701 ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac attatactga aaaccttgct
     5761 tgagaaggtt ttgggacgct cgggtaccag cgtacgagcg acctcatgct atacctgaga
     5821 aagcaacctg acctacagga aagagttact caagaataag aattttcgtt ttaaaaccta
     5881 agagtcactt taaaatttgt atacacttat tttttttata acttatttaa taataaaaat
     5941 cataaatcat aagaaattcg cttatttaga agtgtcaaca acgctacttg tacagctcgt
     6001 ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt
     6061 cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca
     6121 cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca
     6181 gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg
     6241 aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg
     6301 tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca
     6361 cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
     6421 aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt
     6481 acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc ttggtcacct
     6541 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
     6601 actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga
     6661 tggccatgtt atcctcctcg cccttgctca ccatttttct agaaaactta gattagattg
     6721 ctatgctttc tttctaatga gcaagaggta aaaaaagttg tagtagagca agaaaagtgg
     6781 gactgaaact tgggaaattg aaggccgttt attaacttaa atatcgatgg gaggtcatcg
     6841 aaagagaaaa aaatcaaaaa aagaattttc aagaaaaaga aacgtgataa aaatttttat
     6901 tgcctttttc gacgaagaaa aagaaacgag gcggtctctt ttttcttttc caaaccttta
     6961 gtacgggtaa ttaacgacac cctagaggaa gaaagagggg aaatttagta tgctgtgctt
     7021 gggtgttttg aagtggtacg gcgatgcgcg gagtccgaga agatctggaa gagtaaaaga
     7081 ggagtagaaa cgtttcgaag ctatgcatag gccactagtg gatctgatat cacctaataa
     7141 cttcgtatag catacattat acgaagttat attaagggtt ctcgagagct cgggtaccca
     7201 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     7261 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     7321 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     7381 atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7441 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7501 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7561 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7621 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7681 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7741 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7801 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcctgatgcg
     7861 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagggtaat aactgatata
     7921 attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt
     7981 tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt
     8041 caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt
     8101 cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct
     8161 tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac
     8221 ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa
     8281 cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca
     8341 tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa
     8401 tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata
     8461 cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga
     8521 agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg
     8581 aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt
     8641 caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt
     8701 gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac
     8761 gttccttata tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt
     8821 gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat
     8881 ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat
     8941 caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga
     9001 attgaaaagc tgtggtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     9061 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     9121 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     9181 cgtcatcacc gaaacgcgcg a
//
