LOCUS       Exported                8238 bp ds-DNA     circular SYN 07-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 8, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          674..1402
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 bacteriophage origin of replication
                     /label=f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis
     promoter        626..644
                     /label=promoter for bacteriophage T7 RNA polymerase pr
                     /label=promoter for bacteriophage T7 RNA polymerase
     misc_feature    653..673
                     /label=polylinker
     misc_feature    674..685
                     /label=GGSG linker
     CDS             686..1396
                     /codon_start=1
                     /label=NeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     misc_feature    1403..1436
                     /label=FLP recombination target sequence
                     /label=FLP recombination target sequence (FRT)
     misc_feature    1441..1988
                     /label=from the Candida albicans MAL2 gene
     CDS             1995..3266
                     /label=FLP
     misc_feature    3267..3654
                     /label=from the Candida albicans ACT1 gene
     misc_feature    3661..4158
                     /label=from the Candida albicans ACT1 gene
     misc_feature    4169..4822
                     /label=from the Candida albicans Act1 Promoter
                     /label=miscellaneous
     CDS             4823..5385
                     /label=SAT1
     misc_feature    5389..5518
                     /label=from the Candida albicans URA3 gene
     misc_feature    5523..5556
                     /label=FLP recombination target sequence
                     /label=FLP recombination target sequence (FRT)
     misc_feature    5557..5598
                     /label=polylinker
     promoter        complement(5611..5629)
                     /label=promoter for bacteriophage T3 RNA polymerase pr
                     /label=promoter for bacteriophage T3 RNA polymerase
     primer_bind     complement(5650..5666)
                     /label=common sequencing primer, one of multiple simil
                     /label=common sequencing primer, one of multiple similar 
                     variants
     protein_bind    5674..5690
                     /label=The lac repressor binds to the lac operator to
                     /label=The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).
     promoter        complement(5698..5728)
                     /label=promoter for the E. coli lac operon promoter
                     /label=promoter for the E. coli lac operon
     protein_bind    5743..5764
                     /label=CAP binding activates transcription in the pres
                     /label=CAP binding activates transcription in the presence 
                     of cAMP.
     rep_origin      complement(6052..6640)
                     /direction=LEFT
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication
     promoter        6860..6962
                     /label=promoter of the E. coli cat gene encoding chlor
                     /label=promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase
     CDS             6963..7622
                     /label=cat CDS
     promoter        complement(8110..8215)
                     /label=bla promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gagggtggta gtggtatggt ttctaaaggt gaagaagata atatggctag
      721 tttgcctgca acacatgaat tacatatttt tggtagtatt aatggtgttg attttgatat
      781 ggttggtcaa ggtactggta atccaaatga tggttatgaa gaattgaatt tgaaatcaac
      841 taaaggagat ttgcaatttt ctccatggat tttggttcca catattggtt atggtttcca
      901 tcaatatttg ccatatccag atggaatgtc accatttcaa gctgctatgg ttgatggttc
      961 tggttatcaa gttcatagaa ctatgcaatt cgaagatggt gcttcattga ctgttaatta
     1021 tagatatact tatgaaggtt ctcatattaa aggtgaagct caagttaaag gtactggttt
     1081 tccagctgat ggtccagtta tgactaattc attaactgct gctgattggt gtagatcaaa
     1141 gaaaacttat ccaaatgata aaactattat ttcaactttt aaatggtctt atactactgg
     1201 taatggtaaa agatatagat caactgctag aactacttat actttcgcta aaccaatggc
     1261 tgctaattat ttgaaaaatc aaccaatgta tgtttttaga aaaactgaat taaaacattc
     1321 taaaactgaa ttgaatttca aagaatggca aaaagcattc actgatgtta tgggtatgga
     1381 tgaattgtat aaatagctcg aggaagttcc tatactttct agagaatagg aacttcggat
     1441 ccaataatga ttggtttgat atttttgtct agtaccatct gtaccattac acttaaatta
     1501 tctttatatc tgtctaactc gactgtctgg atttcattga tgtagtcgta tgcatcgtta
     1561 gttccaaaaa atattgtcat caatttgata ttggtttccg actctaaaat ttttggaaga
     1621 atttgtctag cgtgctctga gttgtagcca ctgaaaccac ggttaataac atccaatttt
     1681 cggatataca cattctgtaa tgctggatga aagccatact gggtacaact aaactgggtg
     1741 atggagtcac cgaacaacac aaatttaccg tattccatga ttgctatggt tgagaatttt
     1801 ttttttttct tgtcccacgc catttttcaa attatgcagt tgagaatgtt agtttttgtg
     1861 tacaccccgt tcgctgaata tttcggaata attcaaagat tggggagtgg gggaggcgat
     1921 agacgaagac acggtataaa aatgggcaaa attttcccca actttttgca gtggtttaac
     1981 taataatcgt cgacatgcca caatttgata tattatgtaa aacaccacct aaggtgcttg
     2041 ttcgtcagtt tgtggaaagg tttgaaagac cttcaggtga gaaaatagca ttatgtgctg
     2101 ctgaactaac ctatttatgt tggatgatta cacataacgg aacagcaatc aagagagcca
     2161 cattcatgag ctataatact atcataagca attcgttgag tttcgatatt gtcaataaat
     2221 cactccagtt taaatacaag acgcaaaaag caacaatttt ggaagcctca ttaaagaaat
     2281 tgattcctgc ttgggaattt acaattattc cttactatgg acaaaaacat caatctgata
     2341 tcactgatat tgtaagtagt ttgcaattac agttcgaatc atcggaagaa gcagataagg
     2401 gaaatagcca cagtaaaaaa atgcttaaag cacttctaag tgagggtgaa agcatctggg
     2461 agatcactga gaaaatacta aattcgtttg agtatacttc gagatttaca aaaacaaaaa
     2521 ctttatacca attcctcttc ctagctactt tcatcaattg tggaagattc agcgatatta
     2581 agaacgttga tccgaaatca tttaaattag tccaaaataa gtatttggga gtaataatcc
     2641 agtgtttagt gacagagaca aagacaagcg ttagtaggca catatacttc tttagcgcaa
     2701 ggggtaggat cgatccactt gtatatttgg atgaattttt gaggaattct gaaccagtcc
     2761 taaaacgagt aaataggacc ggcaattctt caagcaataa acaggaatac caattattaa
     2821 aagataactt agtcagatcg tacaataaag ctttgaagaa aaatgcgcct tattcaatct
     2881 ttgctataaa aaatggccca aaatctcaca ttggaagaca tttgatgacc tcatttcttt
     2941 caatgaaggg cctaacggag ttgactaatg ttgtgggaaa ttggagcgat aagcgtgctt
     3001 ctgccgtggc caggacaacg tatactcatc agataacagc aatacctgat cactacttcg
     3061 cactagtttc tcggtactat gcatatgatc caatatcaaa ggaaatgata gcattgaagg
     3121 atgagactaa tccaattgag gagtggcagc atatagaaca gctaaagggt agtgctgaag
     3181 gaagcatacg ataccccgca tggaatggga taatatcaca ggaggtacta gactaccttt
     3241 catcctacat aaatagacgc atataagagt gaaattctgg aaatctggaa atctggtttt
     3301 gtattcttgt tattcttctt tttgttatta catatataac ttgttacttt tttaaaaaaa
     3361 tctttgtata ttttataaat atataaaact aaatttaaga aaaagagaaa aatgttttat
     3421 ttgagagatt gaaattttac ttgaatttag cttagctttt ataaagtatt attatgtaaa
     3481 aaaacaaaac aaatatacat taaaaagtta agactataaa atagccaccc aaggcatttc
     3541 tatatcttgt tgttgttgtt ttcatcttct gtatcagagg aacttatttt attattttcg
     3601 tcacgggtat tttctcttgt ttgatgattc atcccattca ttccatcata aaatgtcgag
     3661 cgtcaaaact agagaataat aaagaaaacg atcttttcaa aaagaaaaaa ccttttagtt
     3721 ttcctttgtt gttgttgtgg gtgtgtgcta tttatattat atagtttact cataatacca
     3781 taaaatattc ggtttgatta ggttatttta ataagctaat ttgtttctaa tcgtgtaatt
     3841 tatgctgtgt atattaagta gtgtgtgcac tgcccaaaaa tgtttgttgt ttatagtcgg
     3901 ttaaagagaa aaaagaaaaa aagatccata cacacacgtt aattagttgt tcaacgtaat
     3961 acactcatat tttgttctta tttgctttcg gtcgctgttc tcaccaagat ttattgccaa
     4021 cgaaacaatt tttttttata tattttcaga tttttctttt tttcctttcc tttccttttc
     4081 taattttcac tcctggtttt ctttctttct tagaaacatt atctcgatat taatattaaa
     4141 aaaatataat cattcaaaat ggacggtggt atgttttagt ttagcttcaa ttctaattga
     4201 ttgattaatc agttgattgg tttcaatatg acaaatgggt agggtgggaa aacttcattt
     4261 tcaattcaga tcaaactttt ttgttgtcga cataatattt ctcgtttggg atgttactgt
     4321 cacattaata atacacacac atcagcttat aattttgaaa gtaatttatc agatatgttg
     4381 tgacgatcaa tggaaatggc taacttcaat gtatctgttc ttcccctttt tcaaagttca
     4441 cgttttttga ttgattgatt gatctgtcgg cagtggtttc aaaaccattc ggtgagtaat
     4501 cctatcaatc aatgttacga caaaaggctc aatattcaaa attgcaatgt tttatgtttt
     4561 cctacgtgta cttgtgcaag gcaattgatt caacattgct tttggtgttt gacgagtttc
     4621 tagtttggac ttgtgttgtt atctgggcta tacagatttc ccggctcact atgaattttt
     4681 tttttcgacg ctcagtgcac acaactataa acaacacaaa cacaaacaca gcaagaaaaa
     4741 aaaaaaacga acattgaatt gaaaccaagc caactgaaaa attccttatt taaatgactg
     4801 tcatactaac ccatttttat agaagaagtt gctgctttag ttatcgataa cggttctcat
     4861 atgaaaattt cggtgatccc tgagcaggtg gcggaaacat tggatgctga gaaccatttc
     4921 attgttcgtg aagtgttcga tgtgcaccta tccgaccaag gctttgaact atctaccaga
     4981 agtgtgagcc cctaccggaa ggattacatc tcggatgatg actctgatga agactctgct
     5041 tgctatggcg cattcatcga ccaagagctt gtcgggaaga ttgaactcaa ctcaacatgg
     5101 aacgatctag cctctatcga acacattgtt gtgtcgcaca cgcaccgagg caaaggagtc
     5161 gcgcacagtc tcatcgaatt tgcgaaaaag tgggcactaa gcagacagct ccttggcata
     5221 cgattagaga cacaaacgaa caatgtacct gcctgcaatt tgtacgcaaa atgtggcttt
     5281 actctcggcg gcattgacct cttcacgtat aaaactagac ctcaagtctc gaacgaaaca
     5341 gcgatgtact ggtactggtt ctcgggagca caggatgacg cctaacatat gtgaagtgtg
     5401 aagggggaga ttttcacttt attagatttg tatatatgta taataaataa ataaataagt
     5461 taaataaata attagataag ggtggtaatt attactattt acaatcaaag gtggtcctgc
     5521 aggaagttcc tatactttct agagaatagg aacttcagat ccactagttc tagagcggcc
     5581 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     5641 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5701 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5761 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5821 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5881 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5941 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6001 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6061 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6121 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6181 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6241 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6301 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6361 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6421 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6481 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6541 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6601 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6661 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6721 atcaaaaagg atcttcacct agatcctttt cgaccgaata aatacctgtg acggaagatc
     6781 acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc tgggccaact
     6841 tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa
     6901 taagatcact accgggcgta ttttttgagt tgtcgagatt ttcaggagct aaggaagcta
     6961 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     7021 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     7081 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     7141 ttcacattct tgcccgcctg atgaatgctc atccggaatt acgtatggca atgaaagacg
     7201 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     7261 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     7321 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     7381 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     7441 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     7501 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     7561 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     7621 aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg
     7681 ataataagcg gatgaatggc agaaattcga aagcaaattc gacccggtcg tcggttcagg
     7741 gcagggtcgt taaatagccg cttatgtcta ttgctggttt accggtttat tgactaccgg
     7801 aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag tttgctcagg ctctccccgt
     7861 ggaggtaata attgacgata tgatcctttt tttctgatca aaagtgctca tcattggaaa
     7921 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7981 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8041 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8101 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcaagggt tattgtctca
     8161 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8221 ttccccgaaa agtgccac
//
