LOCUS       pAAV-EF1a-DIO-Ch        7319 bp    DNA     linear       10-MAR-2019
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label=L-ITR
     misc_feature    1450..1483
                     /label=lox2722
     misc_feature    1534..1567
                     /label=loxP
     misc_feature    complement(3551..3584)
                     /label=lox2722
     misc_feature    3683..4291
                     /label=WPRE
     misc_feature    complement(3635..3668)
                     /label=loxP
     misc_feature    4582..4722
                     /label=R-ITR
     rep_origin      4814..5120
                     /label=f1 Origin
     misc_feature    5639..6496
                     /label=AmpR
     rep_origin      6647..7314
                     /label=pUC ori
     misc_feature    1576..1578
                     /note="Construct Stop Codon"
     polyA_signal    4311..4542
                     /dnas_title="SV40 polyA"
                     /vntifkey="25"
                     /label=SV40 polyA
     promoter        156..1419
                     /label=EF-1a
     CDS             complement(1774..2481)
                     /codon_start=1
                     /label=mRuby2
                     /note="mRuby2"
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDG
                     GVVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGY
                     THMALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQRE
                     HAVAKFAGLGGGMDELYK"
     misc_feature    complement(1579..1773)
                     /note="Kv2.1 TS"
     misc_feature    complement(2491..3540)
                     /note="ChrimsonR"
     source          1..7319
                     /dnas_title="pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV21TS-WPRE-SV
                     40"
ORIGIN      
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaG GTACCGGATC CTCTAGAGTC
     1441 GACTCCGGAA TAACTTCGTA TAGGATACTT TATACGAAGT TATGCAGAAT GGTAGCTGGA
     1501 TTGTAGCTGC TATTAGCAAT ATGAAACCTC TTAATAACTT CGTATAGCAT ACATTATACG
     1561 AAGTTATGGC GCGCCTTAga atctggtggc ttcagggaag tccgtggcac agctgatgaa
     1621 gctgtcaatg ctggacatgc tccgcatgtc gatgacgccc tccgtgcgtg cgggcagagg
     1681 ggccacgggg ctgggcatgc tgctcatctc cagctcctct ggaggcttgc tctgcggggc
     1741 catctccttg gtgttgagga tgggctggga ctgcttgtac agctcgtcca tcccaccacc
     1801 aagcccggcg aacttggcaa ctgcgtgttc gcgttgtact acgaacattt cattgtcact
     1861 ttcctctaac ctttccaggc ggtgatcaac ggcatggata ccgggcatct tgatgttccc
     1921 gacggtcttt tttgacctgt aagttgttac gaaagagcaa gacagatggc caccaccatc
     1981 aactttcagt gccatatgag tgtatcccct cagaccacca tctgctggat acatcatctc
     2041 tgtattaggc tcccaaccct tggtcttctt ctgcatcacg ggaccattgg agggaaagtt
     2101 tacccctctg acttggacgt ggtaaacgag acagccatcc tcaaggctgg tgtcctgcat
     2161 gacggtgacg actccaccat cttcgtatct cgtaactctt tcccaagtaa aaccctcagg
     2221 aaaggactgt ttaaagaaat caggaatgcc tttcgggtac ttgataaaag tacggctgcc
     2281 atacatgaac gacgtggcaa gaatgtcaaa ggcaaatggc aggggtcctc cctcgatgac
     2341 tttgatcctc atggtttgag ttcccatgta cggattgcct tctccttcac ctgtgcattt
     2401 gaattggtgg ccgttgaccg aaccttccat gaccaccttc atacgcatat tttccttgat
     2461 cagctcttcg cccttagaca cGGCGGCCGC cactgtgtcc tcgtcctcct cctccacgaa
     2521 ctcttccact tccacctcct cgcctccgat ctccatcttg gtggtcttcc ggatgtcgcc
     2581 gtggatcagg atgtgctcgt ggatcttgat cctcaggtgg tgggccagga aggtccaaaa
     2641 ctccttggcg atgatgtcgc agatgctgtg gccgatgctg ttggcgtaag ggctcagctt
     2701 cagcagtcct tctggtccca ctgcccagag gattgggtag ctgccccaag aggcgaagta
     2761 agcgtaggcc atcagcttca cgaccatgcg gcaatggcct ttaggcacgc tgtggttggc
     2821 ttccacgtag cacttggcgg cctggaagta catgtagccg ccgtagatgc aagacacgat
     2881 atacagcagc cacttgagcc aatcggtagc cagtcctgcg gccatgccga acacgatcat
     2941 tcccacgcaa gacacgatca ggcccatggt ccgcttgctg tagtcgttct tcaggccgct
     3001 caggttgctc agtctgatca ggatcacggg gcaagacagc agccactcga agtagcgcag
     3061 gcaataggcg tggttgccgg tagacaggta cactgtggcg gggctgctga actccttgaa
     3121 gatctccagg gtcacgaaca gcacctcgac gcaacagacg tagacctcct cccaaccgca
     3181 agtggccttc caggcgctga agccgtagaa tgtcagcagg gcgatggcga tgctgaaagc
     3241 aatccactgg cagacctggg cgccgatctt ttctcctggt gtgcctctgc tgtgcagcca
     3301 agcctgcttc ttctccactc ccacgaccag gtagccgcct gtggcctcca cgaagcaata
     3361 tccgtatccg gcgtcgctca gtccgtaaga agggtcacac caccactcgg tgctgaagca
     3421 ctcgcctgta ggtgtcattc ctccacagcc catgtcctcg tggtggtagg ggttaggcca
     3481 agggttgatg cctccggcgg caaacagaga tctggtggcg ctgctgatca gctcagccat
     3541 GGTGGCTAGC ATAACTTCGT ATAAAGTATC CTATACGAAG TTATTTGCCT TAACCCAGAA
     3601 ATTATCACTG TTATTCTTTA GAATGGTGCA AAGAATAACT TCGTATAATG TATGCTATAC
     3661 GAAGTTATGA ATTCGATATC AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG
     3721 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT
     3781 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA
     3841 AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG
     3901 TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC
     3961 TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT
     4021 GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT
     4081 CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG
     4141 GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC
     4201 TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT
     4261 CCCTTTGGGC CGCCTCCCCG CATCGATACC GAGCGCTGCT CGAGAGATCT ttcgagcaga
     4321 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     4381 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4441 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
     4501 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atCTGATTTT GTAGGTAACC
     4561 ACGTGCGGAC CGAGCGGCCG CAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC
     4621 GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC
     4681 GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCTGCCTGCA GGGGCGCCTG ATGCGGTATT
     4741 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACG TCAAAGCAAC CATAGTACGC
     4801 GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC
     4861 ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT
     4921 CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC
     4981 TTTACGGCAC CTCGACCCCA AAAAACTTGA TTTGGGTGAT GGTTCACGTA GTGGGCCATC
     5041 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT
     5101 CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGGC TATTCTTTTG ATTTATAAGG
     5161 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC
     5221 GAATTTTAAC AAAATATTAA CGTTTACAAT TTTATGGTGC ACTCTCAGTA CAATCTGCTC
     5281 TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG
     5341 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT
     5401 GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG
     5461 CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT
     5521 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA
     5581 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT
     5641 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT
     5701 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG
     5761 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA
     5821 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG
     5881 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT
     5941 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG
     6001 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG
     6061 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA
     6121 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC
     6181 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC
     6241 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC
     6301 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG
     6361 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC
     6421 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC
     6481 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT
     6541 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC
     6601 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA
     6661 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC
     6721 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT
     6781 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG
     6841 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC
     6901 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT
     6961 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA
     7021 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT
     7081 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG
     7141 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA
     7201 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA
     7261 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGT
//

